Blood parameters and reproductive hormone profile of female Choloepus hoffmanni in captivity
In wild animal species, the use of non-invasive and non-stressful procedures to determine hormone profiles, such as fecal steroid measurements, has considerably increased the comprehension of their reproductive physiology. Since there is limited information related to the reproductive hormone profil...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | Costa Rica |
| Institución: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| Idioma: | español |
| OAI Identifier: | oai:portal.ucr.ac.cr:article/28448 |
| Acceso en línea: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/28448 |
| Access Level: | acceso abierto |
| Palabra clave: | progesterone estrogen feces estrous cycle hematology sloths AIA-360 progesterona estrógeno heces ciclo estral hematología perezosos |
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Costa Rica |
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Blood parameters and reproductive hormone profile of female Choloepus hoffmanni in captivity Parámetros sanguíneos y perfil de hormonas reproductivas de hembras de Choloepus hoffmanni en cautiverio |
| title |
Blood parameters and reproductive hormone profile of female Choloepus hoffmanni in captivity |
| spellingShingle |
Blood parameters and reproductive hormone profile of female Choloepus hoffmanni in captivity Herra-Vargas, Sofía progesterone estrogen feces estrous cycle hematology sloths AIA-360 progesterona estrógeno heces ciclo estral hematología perezosos AIA-360 |
| title_short |
Blood parameters and reproductive hormone profile of female Choloepus hoffmanni in captivity |
| title_full |
Blood parameters and reproductive hormone profile of female Choloepus hoffmanni in captivity |
| title_fullStr |
Blood parameters and reproductive hormone profile of female Choloepus hoffmanni in captivity |
| title_full_unstemmed |
Blood parameters and reproductive hormone profile of female Choloepus hoffmanni in captivity |
| title_sort |
Blood parameters and reproductive hormone profile of female Choloepus hoffmanni in captivity |
| dc.creator.none.fl_str_mv |
Herra-Vargas, Sofía Brenes-Mora, Esteban Baldi, Mario Bouza-Mora, Laura Huertas-Segura, Rose Mary Castro-Ramírez, Laura Suárez-Esquivel, Marcela |
| author |
Herra-Vargas, Sofía |
| author_facet |
Herra-Vargas, Sofía Brenes-Mora, Esteban Baldi, Mario Bouza-Mora, Laura Huertas-Segura, Rose Mary Castro-Ramírez, Laura Suárez-Esquivel, Marcela |
| author_role |
author |
| author2 |
Brenes-Mora, Esteban Baldi, Mario Bouza-Mora, Laura Huertas-Segura, Rose Mary Castro-Ramírez, Laura Suárez-Esquivel, Marcela |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
progesterone estrogen feces estrous cycle hematology sloths AIA-360 progesterona estrógeno heces ciclo estral hematología perezosos AIA-360 |
| topic |
progesterone estrogen feces estrous cycle hematology sloths AIA-360 progesterona estrógeno heces ciclo estral hematología perezosos AIA-360 |
| description |
In wild animal species, the use of non-invasive and non-stressful procedures to determine hormone profiles, such as fecal steroid measurements, has considerably increased the comprehension of their reproductive physiology. Since there is limited information related to the reproductive hormone profiles of the two-toed sloth, Choloepus hoffmani, a study was conducted in captive specimens at the “Sloth Sanctuary” (Cahuita, Limón, Costa Rica), in order to determine: (i) the reliability of the fecal progesterone and estrogen extraction and its quantification with an AIA-360® analyzer, (ii) assess blood parameters in this species and (iii) evaluate if there is a correlation between fecal and plasmatic steroids. The study was performed over a three-month period, from November, 2013 to January, 2014, with a total amount of 208 fecal samples collected from five sexually mature females weighing 6.32 Kg in average. The average of the median concentrations of progesterone in feces of the five females was 124.21 ng/g, and 1 708.95 pg/g for estrogen. The average minimal and maximal values were 50.96 ng/g and 1 057.46 ng/g for progesterone and, 1 191.77 pg/g and 2 159.24 pg/g for estradiol. In plasma, progesterone median values were 1.26 ng/mL, showing a minimum of 0.32 ng/mL and 12.84 ng/mL as maximum values. The plasmatic estrogen levels were below the detection limit of the equipment (25 pg/mL). Although there was no strong statistical correlation between the fecal and plasmatic progesterone fluctuations, our data suggests that the plasmatic events are mostly reflected in feces two days afterwards. Also, the levels of progesterone were elevated during the first half of November and, subsequently, showed a successive and important reduction in all the females tested. Finally, our results demonstrated that fecal steroid extractions and their measurement in a AIA-360®, allowed the successful detection and represents an alternative non-invasive determination of hormone profiles in C. hoffmani. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-03-01 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion Article artículo original http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article |
| format |
article |
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publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/28448 10.15517/rbt.v66i1.28448 |
| url |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/28448 |
| identifier_str_mv |
10.15517/rbt.v66i1.28448 |
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spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/28448/31943 https://revistas.ucr.ac.cr/index.php/rbt/article/view/28448/31944 https://revistas.ucr.ac.cr/index.php/rbt/article/view/28448/35396 |
| dc.rights.none.fl_str_mv |
Derechos de autor 2018 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 acceso abierto http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Derechos de autor 2018 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 acceso abierto http://purl.org/coar/access_right/c_abf2 |
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openAccess |
| dc.format.none.fl_str_mv |
application/pdf text/html application/pdf |
| dc.publisher.none.fl_str_mv |
Universidad de Costa Rica |
| publisher.none.fl_str_mv |
Universidad de Costa Rica |
| dc.source.none.fl_str_mv |
Revista de Biología Tropical; Vol. 66 No. 1 (2018): Volume 66 – Regular number 1 – March 2018; 280–292 Revista de Biología Tropical; Vol. 66 Núm. 1 (2018): Volumen 66 – Número regular 1 – Marzo 2018; 280–292 Revista Biología Tropical; Vol. 66 N.º 1 (2018): Volumen 66 – Número regular 1 – Marzo 2018; 280–292 2215-2075 0034-7744 10.15517/rbt.v66i1 reponame:Portal de Revistas UCR instname:Universidad de Costa Rica instacron:UCR |
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Universidad de Costa Rica |
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UCR |
| institution |
UCR |
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Portal de Revistas UCR |
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Portal de Revistas UCR |
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Portal de Revistas UCR - Universidad de Costa Rica |
| repository.mail.fl_str_mv |
jorge.polanco@ucr.ac.cr |
| _version_ |
1849325380355227648 |
| spelling |
Blood parameters and reproductive hormone profile of female Choloepus hoffmanni in captivityParámetros sanguíneos y perfil de hormonas reproductivas de hembras de Choloepus hoffmanni en cautiverioHerra-Vargas, SofíaBrenes-Mora, EstebanBaldi, MarioBouza-Mora, LauraHuertas-Segura, Rose MaryCastro-Ramírez, LauraSuárez-Esquivel, Marcelaprogesteroneestrogenfecesestrous cyclehematologyslothsAIA-360progesteronaestrógenohecesciclo estralhematologíaperezososAIA-360In wild animal species, the use of non-invasive and non-stressful procedures to determine hormone profiles, such as fecal steroid measurements, has considerably increased the comprehension of their reproductive physiology. Since there is limited information related to the reproductive hormone profiles of the two-toed sloth, Choloepus hoffmani, a study was conducted in captive specimens at the “Sloth Sanctuary” (Cahuita, Limón, Costa Rica), in order to determine: (i) the reliability of the fecal progesterone and estrogen extraction and its quantification with an AIA-360® analyzer, (ii) assess blood parameters in this species and (iii) evaluate if there is a correlation between fecal and plasmatic steroids. The study was performed over a three-month period, from November, 2013 to January, 2014, with a total amount of 208 fecal samples collected from five sexually mature females weighing 6.32 Kg in average. The average of the median concentrations of progesterone in feces of the five females was 124.21 ng/g, and 1 708.95 pg/g for estrogen. The average minimal and maximal values were 50.96 ng/g and 1 057.46 ng/g for progesterone and, 1 191.77 pg/g and 2 159.24 pg/g for estradiol. In plasma, progesterone median values were 1.26 ng/mL, showing a minimum of 0.32 ng/mL and 12.84 ng/mL as maximum values. The plasmatic estrogen levels were below the detection limit of the equipment (25 pg/mL). Although there was no strong statistical correlation between the fecal and plasmatic progesterone fluctuations, our data suggests that the plasmatic events are mostly reflected in feces two days afterwards. Also, the levels of progesterone were elevated during the first half of November and, subsequently, showed a successive and important reduction in all the females tested. Finally, our results demonstrated that fecal steroid extractions and their measurement in a AIA-360®, allowed the successful detection and represents an alternative non-invasive determination of hormone profiles in C. hoffmani. El uso de técnicas no invasivas ni estresantes para determinar perfiles hormonales, como la medición de esteroides fecales, ha incrementado la comprensión de la fisiología reproductiva en animales silvestres. Debido a la escasa información con respecto a perfiles hormonales reproductivos del perezoso de dos dedos, Choloepus hoffmani, se realizó un estudio en hembras en cautiverio en el centro de rescate “Sloth Sanctuary” (Cahuita, Limón, Costa Rica) con el fin de determinar (i) la confiabilidad de la extracción de progesterona y estradiol en heces, y su cuantificación en el analizador AIA-360®, (ii) evaluar los parámetros sanguíneos en esta especie y (iii) establecer si existe una correlación entre los esteroides plasmáticos y fecales. El estudio se realizó en un periodo de tres meses, durante noviembre de 2013 a enero de 2014, con un total de 208 muestras de heces provenientes de cinco hembras sexualmente maduras, con peso promedio de 6.32 Kg. El promedio de las concentraciones medianas en las heces de las cinco hembras fue 124.21 ng/g para progesterona y 1 708.95 pg/g de estradiol. En plasma, los valores de mediana fueron 1.26 ng/mL con un mínimo de 0.32 ng/mL y 12.84 ng/mL como valor máximo; los valores plasmáticos de estrógeno se encontraron por debajo del límite de detección del equipo (25 pg/mL). Aunque no se encontró una correlación estadísticamente significativa entre la progesterona plasmática y la fecal, nuestros datos sugieren que los eventos plasmáticos se reflejan en heces durante los dos días posteriores. Asimismo, los niveles de progesterona se mantuvieron elevados durante la primera mitad de noviembre, y posteriormente mostraron una reducción importante en todas las hembras. Nuestros resultados demuestran que las extracciones en heces y su medición en el AIA-360® permiten la detección y el seguimiento de variaciones hormonales en C. hoffmani, aunque no remplaza las mediciones plasmáticas para determinar valores absolutos.Universidad de Costa Rica2018-03-01info:eu-repo/semantics/publishedVersionArticleartículo originalhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdftext/htmlapplication/pdfhttps://revistas.ucr.ac.cr/index.php/rbt/article/view/2844810.15517/rbt.v66i1.28448Revista de Biología Tropical; Vol. 66 No. 1 (2018): Volume 66 – Regular number 1 – March 2018; 280–292Revista de Biología Tropical; Vol. 66 Núm. 1 (2018): Volumen 66 – Número regular 1 – Marzo 2018; 280–292Revista Biología Tropical; Vol. 66 N.º 1 (2018): Volumen 66 – Número regular 1 – Marzo 2018; 280–2922215-20750034-774410.15517/rbt.v66i1reponame:Portal de Revistas UCRinstname:Universidad de Costa Ricainstacron:UCRspahttps://revistas.ucr.ac.cr/index.php/rbt/article/view/28448/31943https://revistas.ucr.ac.cr/index.php/rbt/article/view/28448/31944https://revistas.ucr.ac.cr/index.php/rbt/article/view/28448/35396Derechos de autor 2018 Revista de Biología Tropicalhttp://creativecommons.org/licenses/by/4.0acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccess2022-06-09T18:12:57Zoai:portal.ucr.ac.cr:article/28448Portal de revistashttps://revistas.ucr.ac.cr/Universidadhttp://www.ucr.ac.crhttps://revistas.ucr.ac.cr/index.php/index/oaijorge.polanco@ucr.ac.crCosta RicaNo aplicaNo aplicaNo aplicaopendoar:2025-08-13T10:22:59.340Portal de Revistas UCR - Universidad de Costa Ricafalse |
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15,812455 |