Desarrollo de cápsulas y germinación in vitro de Phragmipedium humboldtii, P. longifolium y P. percei
Slipper orchids belonging to the genus Phragmipedium (subfam. Cypripedioideae) are seriously threatened and therefore listed in Appendix I of CITES. Less research has been conducted in this genus than in others belonging to the same subfamily. In this work, we evaluated development of capsules (seed...
| Autores: | , |
|---|---|
| Formato: | artículo |
| Fecha de publicación: | 2007 |
| País: | Costa Rica |
| Recursos: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/77952 |
| Acesso em linha: | https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19529 https://hdl.handle.net/10669/77952 |
| Access Level: | acceso abierto |
| Palavra-chave: | Phragmipedium Orquídeas terrestres Polinización Desarrollo de cápsulas Cloruro de tetrazolio Germinación in vitro 584.4 Orchidales |
| id |
CR_e5d41753510b03f2de3feeedc5e86e0b |
|---|---|
| oai_identifier_str |
oai:kerwa.ucr.ac.cr:10669/77952 |
| network_acronym_str |
CR |
| network_name_str |
Costa Rica |
| repository_id_str |
|
| dc.title.es_ES.fl_str_mv |
Desarrollo de cápsulas y germinación in vitro de Phragmipedium humboldtii, P. longifolium y P. percei |
| title |
Desarrollo de cápsulas y germinación in vitro de Phragmipedium humboldtii, P. longifolium y P. percei |
| spellingShingle |
Desarrollo de cápsulas y germinación in vitro de Phragmipedium humboldtii, P. longifolium y P. percei Muñoz García, Melania Nelly Phragmipedium Orquídeas terrestres Polinización Desarrollo de cápsulas Cloruro de tetrazolio Germinación in vitro 584.4 Orchidales |
| title_short |
Desarrollo de cápsulas y germinación in vitro de Phragmipedium humboldtii, P. longifolium y P. percei |
| title_full |
Desarrollo de cápsulas y germinación in vitro de Phragmipedium humboldtii, P. longifolium y P. percei |
| title_fullStr |
Desarrollo de cápsulas y germinación in vitro de Phragmipedium humboldtii, P. longifolium y P. percei |
| title_full_unstemmed |
Desarrollo de cápsulas y germinación in vitro de Phragmipedium humboldtii, P. longifolium y P. percei |
| title_sort |
Desarrollo de cápsulas y germinación in vitro de Phragmipedium humboldtii, P. longifolium y P. percei |
| dc.creator.none.fl_str_mv |
Muñoz García, Melania Nelly Jiménez García, Víctor |
| author |
Muñoz García, Melania Nelly |
| author_facet |
Muñoz García, Melania Nelly Jiménez García, Víctor |
| author_role |
author |
| author2 |
Jiménez García, Víctor |
| author2_role |
author |
| dc.subject.es_ES.fl_str_mv |
Phragmipedium Orquídeas terrestres Polinización Desarrollo de cápsulas Cloruro de tetrazolio Germinación in vitro 584.4 Orchidales |
| topic |
Phragmipedium Orquídeas terrestres Polinización Desarrollo de cápsulas Cloruro de tetrazolio Germinación in vitro 584.4 Orchidales |
| description |
Slipper orchids belonging to the genus Phragmipedium (subfam. Cypripedioideae) are seriously threatened and therefore listed in Appendix I of CITES. Less research has been conducted in this genus than in others belonging to the same subfamily. In this work, we evaluated development of capsules (seed pods) from Phragmipedium humboldtii, P. longifolium and P. peaarcei from the time of pollination until opening. Moreover, seed viability was tested with the tetrazolium method in each of the capsules that were subsequently used to evaluate the effect of light and two culture media (Knudson C vs. Murashige and Skoog half concentrated) on in vitro asymbiotic germination and seedling growth. 100% of the pollinated flowers developed capsules, which differentiated in terms of length and diameter among species. While the length of the capsules remained constant during development, their diameter increased during the first 6-8 weeks and then stopped. Time required for maturity and opening of the capsules also varied among species (31 weeks in P. humboldtii, 16 weeks in P. longifolium and 9.5 weeks in P. percei). Seed viability differed among species as well, averaging 34.3% in P. humboldtii, 44.7% in P. longifolium and 82.3% in P. pearcei. Furthermore, seed viability of each capsule was used to adjust the germination rate measured in each case. While very few P. humboldtii viable seeds germinated under the conditions tested (2.9%), better results were observed in the other two species (close to 40% germination). No significant effect of light/darkness regime or of culture medium was observed on germination. However, better growth of the germinated embryos was observed with the Knudson C medium and darkness conditions. Further subculture of the gro- wing plantlets under light conditions induced development of roots and allowed successful acclimatization of seedlings in the greenhouse. |
| publishDate |
2007 |
| dc.date.issued.none.fl_str_mv |
2007-06 |
| dc.date.accessioned.none.fl_str_mv |
2019-06-28T19:49:29Z |
| dc.date.available.none.fl_str_mv |
2019-06-28T19:49:29Z |
| dc.date.updated.none.fl_str_mv |
2019-06-09T20:10:33Z |
| dc.type.none.fl_str_mv |
artículo original http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.citation.none.fl_str_mv |
https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19529 |
| dc.identifier.issn.none.fl_str_mv |
2215-2067 |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/10669/77952 |
| dc.identifier.doi.none.fl_str_mv |
10.15517/lank.v7i1-2.19529 |
| dc.identifier.codproyecto.none.fl_str_mv |
814-A6-107 |
| url |
https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19529 https://hdl.handle.net/10669/77952 |
| identifier_str_mv |
2215-2067 10.15517/lank.v7i1-2.19529 814-A6-107 |
| dc.language.iso.es_ES.fl_str_mv |
es |
| language_invalid_str_mv |
es |
| dc.rights.none.fl_str_mv |
acceso abierto http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
acceso abierto http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.source.es_ES.fl_str_mv |
Lankesteriana; vol.7(1-2), pp. 310-312 |
| dc.source.none.fl_str_mv |
reponame:Kérwá instname:Universidad de Costa Rica instacron:UCR |
| instname_str |
Universidad de Costa Rica |
| instacron_str |
UCR |
| institution |
UCR |
| reponame_str |
Kérwá |
| collection |
Kérwá |
| bitstream.url.fl_str_mv |
https://www.kerwa.ucr.ac.cr/bitstreams/734b3a3f-c77b-48de-8b68-f9431c31b680/download https://www.kerwa.ucr.ac.cr/bitstreams/956435e4-64bd-4e6e-8553-fe5b8ab1704d/download https://www.kerwa.ucr.ac.cr/bitstreams/efc70a3b-8092-43c8-a54b-4ac443308a72/download https://www.kerwa.ucr.ac.cr/bitstreams/e9bde4ff-9d0e-4807-baca-b09580a54f82/download https://www.kerwa.ucr.ac.cr/bitstreams/fac62cb8-b755-4db1-a335-f61a747d18f5/download |
| bitstream.checksum.fl_str_mv |
13f39f72a41812e9d897aeb2f88179b5 d41d8cd98f00b204e9800998ecf8427e 0a6d12fd4aa0cb355b936571713dc67d 9e329a771bd212772ffb63a7dcb3a006 fc593b1884428c6488e3e19e54bffdbd |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Kérwá - Universidad de Costa Rica |
| repository.mail.fl_str_mv |
meilyn.garro@ucr.ac.cr |
| _version_ |
1849325593283264512 |
| spelling |
Muñoz García, Melania Nelly6e17ff32-39eb-4390-b188-97f54cdb41a0600Jiménez García, Víctorc4446754-8301-48fe-af11-dcd552a207956002019-06-28T19:49:29Z2019-06-28T19:49:29Z2007-062019-06-09T20:10:33Zhttps://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/195292215-2067https://hdl.handle.net/10669/7795210.15517/lank.v7i1-2.19529814-A6-107Slipper orchids belonging to the genus Phragmipedium (subfam. Cypripedioideae) are seriously threatened and therefore listed in Appendix I of CITES. Less research has been conducted in this genus than in others belonging to the same subfamily. In this work, we evaluated development of capsules (seed pods) from Phragmipedium humboldtii, P. longifolium and P. peaarcei from the time of pollination until opening. Moreover, seed viability was tested with the tetrazolium method in each of the capsules that were subsequently used to evaluate the effect of light and two culture media (Knudson C vs. Murashige and Skoog half concentrated) on in vitro asymbiotic germination and seedling growth. 100% of the pollinated flowers developed capsules, which differentiated in terms of length and diameter among species. While the length of the capsules remained constant during development, their diameter increased during the first 6-8 weeks and then stopped. Time required for maturity and opening of the capsules also varied among species (31 weeks in P. humboldtii, 16 weeks in P. longifolium and 9.5 weeks in P. percei). Seed viability differed among species as well, averaging 34.3% in P. humboldtii, 44.7% in P. longifolium and 82.3% in P. pearcei. Furthermore, seed viability of each capsule was used to adjust the germination rate measured in each case. While very few P. humboldtii viable seeds germinated under the conditions tested (2.9%), better results were observed in the other two species (close to 40% germination). No significant effect of light/darkness regime or of culture medium was observed on germination. However, better growth of the germinated embryos was observed with the Knudson C medium and darkness conditions. Further subculture of the gro- wing plantlets under light conditions induced development of roots and allowed successful acclimatization of seedlings in the greenhouse.Universidad de Costa Rica/[814-A6-107]/UCR/Costa RicaUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Centro para Investigaciones en Granos y Semillas (CIGRAS)UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Jardín Botánico Lankester (JBL)esacceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessLankesteriana; vol.7(1-2), pp. 310-312reponame:Kérwáinstname:Universidad de Costa Ricainstacron:UCRPhragmipediumOrquídeas terrestresPolinizaciónDesarrollo de cápsulasCloruro de tetrazolioGerminación in vitro584.4 OrchidalesDesarrollo de cápsulas y germinación in vitro de Phragmipedium humboldtii, P. longifolium y P. perceiartículo originalhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleORIGINAL2007 - Phragmipedium (IOCC).pdfapplication/pdf864086https://www.kerwa.ucr.ac.cr/bitstreams/734b3a3f-c77b-48de-8b68-f9431c31b680/download13f39f72a41812e9d897aeb2f88179b5MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-80https://www.kerwa.ucr.ac.cr/bitstreams/956435e4-64bd-4e6e-8553-fe5b8ab1704d/downloadd41d8cd98f00b204e9800998ecf8427eMD52SWORD356677957.zipSWORD deposit packageapplication/octet-stream412781https://www.kerwa.ucr.ac.cr/bitstreams/efc70a3b-8092-43c8-a54b-4ac443308a72/download0a6d12fd4aa0cb355b936571713dc67dMD53TEXT2007 - Phragmipedium (IOCC).pdf.txt2007 - Phragmipedium (IOCC).pdf.txtExtracted texttext/plain11709https://www.kerwa.ucr.ac.cr/bitstreams/e9bde4ff-9d0e-4807-baca-b09580a54f82/download9e329a771bd212772ffb63a7dcb3a006MD54THUMBNAIL2007 - Phragmipedium (IOCC).pdf.jpg2007 - Phragmipedium (IOCC).pdf.jpgGenerated Thumbnailimage/jpeg4696https://www.kerwa.ucr.ac.cr/bitstreams/fac62cb8-b755-4db1-a335-f61a747d18f5/downloadfc593b1884428c6488e3e19e54bffdbdMD5510669/779522024-05-29 07:47:24.26open.accessoai:kerwa.ucr.ac.cr:10669/77952https://www.kerwa.ucr.ac.crInstitucionalhttp://www.kerwa.ucr.ac.crUniversidadhttp://www.ucr.ac.crhttps://kerwa.ucr.ac.cr/oai/requestmeilyn.garro@ucr.ac.crCosta RicaNo aplicaNo aplicaNo aplicaopendoar:18712025-03-29T18:00:48.610Kérwá - Universidad de Costa Ricafalse |
| score |
15,228081 |