Seasonal behavior of Thalassia testudinum (Hydrocharitaceae) metabolites
The marine angiosperm Thalassia testudinum, commonly known as turtle grass, is a dominant seagrass that grows in the Caribbean Sea shelf associated to Syringodium filiforme. The hydroalcoholic extract of T. testudinum is rich in polyphenols; the most abundant metabolite in this extract is thalassiol...
| Autores: | , , , , , , , , |
|---|---|
| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2016 |
| País: | Costa Rica |
| Recursos: | Universidad de Costa Rica |
| Repositório: | Portal de Revistas UCR |
| Idioma: | inglês |
| OAI Identifier: | oai:portal.ucr.ac.cr:article/21037 |
| Acesso em linha: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/21037 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Thalassia testudinum seagrass seasonal variation polyphenols antioxidant activity. Angiosperma variación estacional polifenoles actividad antioxidante. |
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Seasonal behavior of Thalassia testudinum (Hydrocharitaceae) metabolites Comportamiento estacional de los metabolitos de Thalassia testudinum (Hydrocharital: Hydrocharitaceae) |
| title |
Seasonal behavior of Thalassia testudinum (Hydrocharitaceae) metabolites |
| spellingShingle |
Seasonal behavior of Thalassia testudinum (Hydrocharitaceae) metabolites Hernández Rivera, Yasnay Thalassia testudinum seagrass seasonal variation polyphenols antioxidant activity. Angiosperma Thalassia testudinum variación estacional polifenoles actividad antioxidante. |
| title_short |
Seasonal behavior of Thalassia testudinum (Hydrocharitaceae) metabolites |
| title_full |
Seasonal behavior of Thalassia testudinum (Hydrocharitaceae) metabolites |
| title_fullStr |
Seasonal behavior of Thalassia testudinum (Hydrocharitaceae) metabolites |
| title_full_unstemmed |
Seasonal behavior of Thalassia testudinum (Hydrocharitaceae) metabolites |
| title_sort |
Seasonal behavior of Thalassia testudinum (Hydrocharitaceae) metabolites |
| dc.creator.none.fl_str_mv |
Hernández Rivera, Yasnay González García, Kethia Valdés-Iglesias, Olga del Rosario Zarabozo Silva, Akaena Portal Ríos, Yessica Laguna Granja, Abilio Martínez Daranas, Beatriz Rodríguez García, María Gutiérrez Cuesta, Richard |
| author |
Hernández Rivera, Yasnay |
| author_facet |
Hernández Rivera, Yasnay González García, Kethia Valdés-Iglesias, Olga del Rosario Zarabozo Silva, Akaena Portal Ríos, Yessica Laguna Granja, Abilio Martínez Daranas, Beatriz Rodríguez García, María Gutiérrez Cuesta, Richard |
| author_role |
author |
| author2 |
González García, Kethia Valdés-Iglesias, Olga del Rosario Zarabozo Silva, Akaena Portal Ríos, Yessica Laguna Granja, Abilio Martínez Daranas, Beatriz Rodríguez García, María Gutiérrez Cuesta, Richard |
| author2_role |
author author author author author author author author |
| dc.subject.none.fl_str_mv |
Thalassia testudinum seagrass seasonal variation polyphenols antioxidant activity. Angiosperma Thalassia testudinum variación estacional polifenoles actividad antioxidante. |
| topic |
Thalassia testudinum seagrass seasonal variation polyphenols antioxidant activity. Angiosperma Thalassia testudinum variación estacional polifenoles actividad antioxidante. |
| description |
The marine angiosperm Thalassia testudinum, commonly known as turtle grass, is a dominant seagrass that grows in the Caribbean Sea shelf associated to Syringodium filiforme. The hydroalcoholic extract of T. testudinum is rich in polyphenols; the most abundant metabolite in this extract is thalassiolin B, a glycosilated flavonoid with skin damage repairing properties, and antioxidant capacity among others. The present study aimed at generating information about the seasonal behavior of secondary metabolites, as well as to study the antioxidant capacity of the T. testudinum leaves extract, collected monthly during 2012 from the Northeast coastline of Havana, Cuba. For this study, spectrophotometric methods were used to determine the concentrations of polyphenols, flavonoids, anthocyanins, soluble carbohydrates and proteins, chlorophylls a and b, and antioxidant activity of the extracts. In general, results demonstrated seasonal variations of the analyzed parameters. Extracts prepared from the vegetal material collected in October and November showed the highest values of polyphenols (58.81 ± 1.53 and 52.39 ± 0.63 mg/g bs, respectivally) and flavonoids (44.12 ± 1.30 and 51.30 ± 0.67 mg/gdw, respectively). On the contrary, the lowest values of polyphenols were found in extracts of leaves collected in July and August (15.51 ± 0.84 and 13.86 ± 0.48 mg/g,respectively). In accordance with these results, the lower value of Inhibitory Concentration (IC50) was obtained to get a 50 % of maximal effect on free radical scavenging activity with the extracts prepared from leaves collected in October and November, and less significant IC50 was obtained from the extract prepared from leaves collected in August (5.63 mg/mL). A negative correlation (r= -0.694) was observed in this study between the content of polyphenols and the IC50 necessary to get the half of its antioxidant maximal effect. The high correspondence between the maximum values of polyphenols, flavonoids, carbohydrates and proteins in October and November, revealed a close relationship between these metabolites found in the extract of T. testudinum. Our hypothesis about the annual variation in the concentration of these metabolites was validated; and these results will support the correct harvesting of T. testudinum leaves for biotechnology and industrial purposes. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016-12-01 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion Article artículo original http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/21037 10.15517/rbt.v64i4.21037 |
| url |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/21037 |
| identifier_str_mv |
10.15517/rbt.v64i4.21037 |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/21037/26645 https://revistas.ucr.ac.cr/index.php/rbt/article/view/21037/26646 |
| dc.rights.none.fl_str_mv |
Derechos de autor 2016 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 acceso abierto http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
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Derechos de autor 2016 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 acceso abierto http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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text/html application/pdf |
| dc.publisher.none.fl_str_mv |
Universidad de Costa Rica |
| publisher.none.fl_str_mv |
Universidad de Costa Rica |
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Revista de Biología Tropical; Vol. 64 No. 4 (2016): Volume 64 – Regular number 4 – December 2016; 1527–1535 Revista de Biología Tropical; Vol. 64 Núm. 4 (2016): Volumen 64 – Número regular 4 – Diciembre 2016; 1527–1535 Revista Biología Tropical; Vol. 64 N.º 4 (2016): Volumen 64 – Número regular 4 – Diciembre 2016; 1527–1535 2215-2075 0034-7744 10.15517/rbt.v64i4 reponame:Portal de Revistas UCR instname:Universidad de Costa Rica instacron:UCR |
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Universidad de Costa Rica |
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UCR |
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UCR |
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Portal de Revistas UCR |
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Portal de Revistas UCR |
| repository.name.fl_str_mv |
Portal de Revistas UCR - Universidad de Costa Rica |
| repository.mail.fl_str_mv |
jorge.polanco@ucr.ac.cr |
| _version_ |
1849325368111005696 |
| spelling |
Seasonal behavior of Thalassia testudinum (Hydrocharitaceae) metabolitesComportamiento estacional de los metabolitos de Thalassia testudinum (Hydrocharital: Hydrocharitaceae)Hernández Rivera, YasnayGonzález García, KethiaValdés-Iglesias, Olga del RosarioZarabozo Silva, AkaenaPortal Ríos, YessicaLaguna Granja, AbilioMartínez Daranas, BeatrizRodríguez García, MaríaGutiérrez Cuesta, RichardThalassia testudinumseagrassseasonal variationpolyphenolsantioxidant activity.AngiospermaThalassia testudinumvariación estacionalpolifenolesactividad antioxidante.The marine angiosperm Thalassia testudinum, commonly known as turtle grass, is a dominant seagrass that grows in the Caribbean Sea shelf associated to Syringodium filiforme. The hydroalcoholic extract of T. testudinum is rich in polyphenols; the most abundant metabolite in this extract is thalassiolin B, a glycosilated flavonoid with skin damage repairing properties, and antioxidant capacity among others. The present study aimed at generating information about the seasonal behavior of secondary metabolites, as well as to study the antioxidant capacity of the T. testudinum leaves extract, collected monthly during 2012 from the Northeast coastline of Havana, Cuba. For this study, spectrophotometric methods were used to determine the concentrations of polyphenols, flavonoids, anthocyanins, soluble carbohydrates and proteins, chlorophylls a and b, and antioxidant activity of the extracts. In general, results demonstrated seasonal variations of the analyzed parameters. Extracts prepared from the vegetal material collected in October and November showed the highest values of polyphenols (58.81 ± 1.53 and 52.39 ± 0.63 mg/g bs, respectivally) and flavonoids (44.12 ± 1.30 and 51.30 ± 0.67 mg/gdw, respectively). On the contrary, the lowest values of polyphenols were found in extracts of leaves collected in July and August (15.51 ± 0.84 and 13.86 ± 0.48 mg/g,respectively). In accordance with these results, the lower value of Inhibitory Concentration (IC50) was obtained to get a 50 % of maximal effect on free radical scavenging activity with the extracts prepared from leaves collected in October and November, and less significant IC50 was obtained from the extract prepared from leaves collected in August (5.63 mg/mL). A negative correlation (r= -0.694) was observed in this study between the content of polyphenols and the IC50 necessary to get the half of its antioxidant maximal effect. The high correspondence between the maximum values of polyphenols, flavonoids, carbohydrates and proteins in October and November, revealed a close relationship between these metabolites found in the extract of T. testudinum. Our hypothesis about the annual variation in the concentration of these metabolites was validated; and these results will support the correct harvesting of T. testudinum leaves for biotechnology and industrial purposes.La angiosperma marina Thalassia testudinum, comúnmente conocida como "hierba tortuga", es un pasto marino dominante que crece en el Mar Caribe asociada a Syringodium filiforme. El extracto hidroalcohólico de T. testudinum es rico en polifenoles; el metabolito más abundante en este extracto es thalassiolina B, un flavonoide glicosilado con propiedades para la reparación de daños en la piel y la capacidad antioxidante, entre otros. El objetivo del presente estudio fue conocer el comportamiento estacional de los metabolitos secundarios, así como la capacidad antioxidante del extracto de hojas de T. testudinum recolectadas mensualmente durante el 2012, en la costa noreste de La Habana, Cuba. Para este estudio fueron empleados métodos espectrofotométricos para la determinación de la concentración de polifenoles, flavonoides, carbohidratos y proteínas solubles, clorofilas a y b y la actividad antioxidante del extracto. En sentido general, los resultados demostraron las variaciones estacionales de los parámetros analizados; los extractos preparados a partir de material vegetal recolectado en octubre y noviembre mostraron los mayores valores de polifenoles y flavonoides (44.12 ± 1.30 y 51.30 ± 0.67 mg/gbs respectivamente) y se encontraron los valores más bajos en los extractos de hojas recolectadas en julio y agosto. (15.51 ± 0.84 y 13.86 ± 0.48 mg/grespectivamente). De acuerdo con los resultados, se obtuvo el valor más bajo de la concentración inhibitoria (CI50 ) necesaria para obtener un 50 % de efecto máximo en la actividad de captación de radicales libres con los extractos preparados a partir de las hojas recolectadas en octubre y noviembre y la CI50 menos significativa se obtuvo a partir del extracto preparado a partir de las hojas recolectadas en agosto (5.63 mg/ml). Se observó una correlación negativa (r= -0,694) entre el contenido de polifenoles y la CI50 necesaria para obtener la mitad del efecto antioxidante máximo. La alta correspondencia que existe entre los valores máximos de polifenoles, flavonoides, carbohidratos y proteínas en octubre y noviembre reveló una estrecha relación entre estos metabolitos, encontrados en el extracto de T. testudinum. La hipótesis de la variación anual de la concentración de estos metabolitos fue validada. Estos resultados se tendrán en cuenta con el fin de seleccionar el momento de recolecta de las hojas de T. testudinum para su uso con fines biotecnológicos e industriales.Universidad de Costa Rica2016-12-01info:eu-repo/semantics/publishedVersionArticleartículo originalhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articletext/htmlapplication/pdfhttps://revistas.ucr.ac.cr/index.php/rbt/article/view/2103710.15517/rbt.v64i4.21037Revista de Biología Tropical; Vol. 64 No. 4 (2016): Volume 64 – Regular number 4 – December 2016; 1527–1535Revista de Biología Tropical; Vol. 64 Núm. 4 (2016): Volumen 64 – Número regular 4 – Diciembre 2016; 1527–1535Revista Biología Tropical; Vol. 64 N.º 4 (2016): Volumen 64 – Número regular 4 – Diciembre 2016; 1527–15352215-20750034-774410.15517/rbt.v64i4reponame:Portal de Revistas UCRinstname:Universidad de Costa Ricainstacron:UCRenghttps://revistas.ucr.ac.cr/index.php/rbt/article/view/21037/26645https://revistas.ucr.ac.cr/index.php/rbt/article/view/21037/26646Derechos de autor 2016 Revista de Biología Tropicalhttp://creativecommons.org/licenses/by/4.0acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccess2022-06-09T17:58:04Zoai:portal.ucr.ac.cr:article/21037Portal de revistashttps://revistas.ucr.ac.cr/Universidadhttp://www.ucr.ac.crhttps://revistas.ucr.ac.cr/index.php/index/oaijorge.polanco@ucr.ac.crCosta RicaNo aplicaNo aplicaNo aplicaopendoar:2025-08-13T10:18:57.168Portal de Revistas UCR - Universidad de Costa Ricafalse |
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15,198674 |