Potentially pathogenic Acanthamoeba genotype T4 isolated from dental units and emergency combination showers

Background: Acanthamoeba is the genus of free-living amoebae that is most frequently isolated in nature. To date, 20 Acanthamoeba genotypes have been described. Genotype T4 is responsible for approximately 90% of encephalitis and keratitis cases. Due to the ubiquitous presence of amoebae, isolation...

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Detalhes bibliográficos
Autores: Castro Artavia, Esteban, Retana Moreira, Lissette, Lorenzo Morales, Jacob, Abrahams Sandí, Elizabeth
Formato: artículo
Fecha de publicación:2017
País:Costa Rica
Recursos:Universidad de Costa Rica
Repositorio:Kérwá
Idioma:inglés
OAI Identifier:oai:kerwa.ucr.ac.cr:10669/88174
Acesso em linha:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5719550/pdf/0074-0276-mioc-112-12-0817.pdf
https://memorias.ioc.fiocruz.br/article/6341/0147_potentially-pathogenic-acanthamoeba-genotype-t4-isolated-from-dental-units-and-emergency-combination-showers
https://hdl.handle.net/10669/88174
Access Level:acceso abierto
Palavra-chave:Acanthamoeba genotype T4
Free-living amoeba
Dental unit
Emergency combination showers
GEN
MEDICAL SCIENCES
CELL BIOLOGY
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repository_id_str
dc.title.es_ES.fl_str_mv Potentially pathogenic Acanthamoeba genotype T4 isolated from dental units and emergency combination showers
title Potentially pathogenic Acanthamoeba genotype T4 isolated from dental units and emergency combination showers
spellingShingle Potentially pathogenic Acanthamoeba genotype T4 isolated from dental units and emergency combination showers
Castro Artavia, Esteban
Acanthamoeba genotype T4
Free-living amoeba
Dental unit
Emergency combination showers
GEN
MEDICAL SCIENCES
CELL BIOLOGY
title_short Potentially pathogenic Acanthamoeba genotype T4 isolated from dental units and emergency combination showers
title_full Potentially pathogenic Acanthamoeba genotype T4 isolated from dental units and emergency combination showers
title_fullStr Potentially pathogenic Acanthamoeba genotype T4 isolated from dental units and emergency combination showers
title_full_unstemmed Potentially pathogenic Acanthamoeba genotype T4 isolated from dental units and emergency combination showers
title_sort Potentially pathogenic Acanthamoeba genotype T4 isolated from dental units and emergency combination showers
dc.creator.none.fl_str_mv Castro Artavia, Esteban
Retana Moreira, Lissette
Lorenzo Morales, Jacob
Abrahams Sandí, Elizabeth
author Castro Artavia, Esteban
author_facet Castro Artavia, Esteban
Retana Moreira, Lissette
Lorenzo Morales, Jacob
Abrahams Sandí, Elizabeth
author_role author
author2 Retana Moreira, Lissette
Lorenzo Morales, Jacob
Abrahams Sandí, Elizabeth
author2_role author
author
author
dc.subject.es_ES.fl_str_mv Acanthamoeba genotype T4
Free-living amoeba
Dental unit
Emergency combination showers
GEN
MEDICAL SCIENCES
CELL BIOLOGY
topic Acanthamoeba genotype T4
Free-living amoeba
Dental unit
Emergency combination showers
GEN
MEDICAL SCIENCES
CELL BIOLOGY
description Background: Acanthamoeba is the genus of free-living amoebae that is most frequently isolated in nature. To date, 20 Acanthamoeba genotypes have been described. Genotype T4 is responsible for approximately 90% of encephalitis and keratitis cases. Due to the ubiquitous presence of amoebae, isolation from environmental sources is not uncommon; to determine the clinical importance of an isolation, it is necessary to have evidence of the pathogenic potential of amoebae. Objective: The aim of this study was to physiologically characterise 8 Acanthamoeba T4 isolates obtained from dental units and emergency combination showers and to determine their pathogenic potential by employing different laboratory techniques. Methods: Eight axenic cultures of Acanthamoeba genotype T4 were used in pathogenic potential assays. Osmotolerance, thermotolerance, determination and characterisation of extracellular proteases and evaluation of cytopathic effects in MDCK cells were performed. Findings: All of the isolates were osmotolerant, thermotolerant and had serine proteases from 44-122 kDa. Two isolates had cytopathic effects on the MDCK cell monolayer. Main conclusion: The presence of Acanthamoeba T4 with pathogenic potential in areas such as those tested in this study reaffirms the need for adequate cleaning and maintenance protocols to reduce the possibility of infection with free-living amoebae
publishDate 2017
dc.date.issued.none.fl_str_mv 2017-12
dc.date.accessioned.none.fl_str_mv 2023-02-10T16:44:41Z
dc.date.available.none.fl_str_mv 2023-02-10T16:44:41Z
dc.type.es_ES.fl_str_mv artículo original
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
info:eu-repo/semantics/article
format article
dc.identifier.citation.es_ES.fl_str_mv https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5719550/pdf/0074-0276-mioc-112-12-0817.pdf
https://memorias.ioc.fiocruz.br/article/6341/0147_potentially-pathogenic-acanthamoeba-genotype-t4-isolated-from-dental-units-and-emergency-combination-showers
dc.identifier.issn.none.fl_str_mv 1678-8060
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10669/88174
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760170147
dc.identifier.codproyecto.none.fl_str_mv 803-B4-050
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5719550/pdf/0074-0276-mioc-112-12-0817.pdf
https://memorias.ioc.fiocruz.br/article/6341/0147_potentially-pathogenic-acanthamoeba-genotype-t4-isolated-from-dental-units-and-emergency-combination-showers
https://hdl.handle.net/10669/88174
identifier_str_mv 1678-8060
10.1590/0074-02760170147
803-B4-050
dc.language.iso.es_ES.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv acceso abierto
http://purl.org/coar/access_right/c_abf2
info:eu-repo/semantics/openAccess
rights_invalid_str_mv acceso abierto
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eu_rights_str_mv openAccess
dc.source.es_ES.fl_str_mv Memorias do Instituto Oswaldo Cruz, 112(12), p. 817-821.
dc.source.none.fl_str_mv reponame:Kérwá
instname:Universidad de Costa Rica
instacron:UCR
instname_str Universidad de Costa Rica
instacron_str UCR
institution UCR
reponame_str Kérwá
collection Kérwá
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spelling Castro Artavia, Estebana3ec11e4-0be0-46c3-a0c8-a6e0f9d27884600Retana Moreira, Lissetteb78e1970-41f4-4602-9893-11629325034e600Lorenzo Morales, Jacobd8ed3477-4346-4b4e-b47f-786cccf792d6Abrahams Sandí, Elizabeth0779e8b1-07d7-408a-b5b4-d68d7d85e8676002023-02-10T16:44:41Z2023-02-10T16:44:41Z2017-12https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5719550/pdf/0074-0276-mioc-112-12-0817.pdfhttps://memorias.ioc.fiocruz.br/article/6341/0147_potentially-pathogenic-acanthamoeba-genotype-t4-isolated-from-dental-units-and-emergency-combination-showers1678-8060https://hdl.handle.net/10669/8817410.1590/0074-02760170147803-B4-050Background: Acanthamoeba is the genus of free-living amoebae that is most frequently isolated in nature. To date, 20 Acanthamoeba genotypes have been described. Genotype T4 is responsible for approximately 90% of encephalitis and keratitis cases. Due to the ubiquitous presence of amoebae, isolation from environmental sources is not uncommon; to determine the clinical importance of an isolation, it is necessary to have evidence of the pathogenic potential of amoebae. Objective: The aim of this study was to physiologically characterise 8 Acanthamoeba T4 isolates obtained from dental units and emergency combination showers and to determine their pathogenic potential by employing different laboratory techniques. Methods: Eight axenic cultures of Acanthamoeba genotype T4 were used in pathogenic potential assays. Osmotolerance, thermotolerance, determination and characterisation of extracellular proteases and evaluation of cytopathic effects in MDCK cells were performed. Findings: All of the isolates were osmotolerant, thermotolerant and had serine proteases from 44-122 kDa. Two isolates had cytopathic effects on the MDCK cell monolayer. Main conclusion: The presence of Acanthamoeba T4 with pathogenic potential in areas such as those tested in this study reaffirms the need for adequate cleaning and maintenance protocols to reduce the possibility of infection with free-living amoebaeUniversidad de Costa RicaUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Centro de Investigación en Enfermedades Tropicales (CIET)UCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiologíaengacceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessMemorias do Instituto Oswaldo Cruz, 112(12), p. 817-821.reponame:Kérwáinstname:Universidad de Costa Ricainstacron:UCRAcanthamoeba genotype T4Free-living amoebaDental unitEmergency combination showersGENMEDICAL SCIENCESCELL BIOLOGYPotentially pathogenic Acanthamoeba genotype T4 isolated from dental units and emergency combination showersartículo originalhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleORIGINALAcanthamoeba unidades dentales y duchas 2017.pdfAcanthamoeba unidades dentales y duchas 2017.pdfapplication/pdf505910https://www.kerwa.ucr.ac.cr/bitstreams/fb8b4215-d8ac-44a0-b78b-9142852af40d/download79336cd66921aec7be85de7e093ba866MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-83584https://www.kerwa.ucr.ac.cr/bitstreams/d459c7b5-69a5-40f6-a742-4ce60a922202/downloadb39c1930822b6ee5a194051b92f3a43eMD53TEXTAcanthamoeba unidades dentales y duchas 2017.pdf.txtAcanthamoeba unidades dentales y duchas 2017.pdf.txtExtracted texttext/plain24573https://www.kerwa.ucr.ac.cr/bitstreams/75e4ab3c-f51a-41bc-a076-5968ef2bd57f/download74f32cacb318dafd928f9292c9893a5eMD54THUMBNAILAcanthamoeba unidades dentales y duchas 2017.pdf.jpgAcanthamoeba unidades dentales y duchas 2017.pdf.jpgIM Thumbnailimage/jpeg7442https://www.kerwa.ucr.ac.cr/bitstreams/c892456e-e4e9-4d00-9325-2bf720921463/download73f146e8148bcd9a9fa873597ca09459MD5510669/881742024-08-22 05:11:25.833open.accessoai:kerwa.ucr.ac.cr:10669/88174https://www.kerwa.ucr.ac.crInstitucionalhttp://www.kerwa.ucr.ac.crUniversidadhttp://www.ucr.ac.crhttps://kerwa.ucr.ac.cr/oai/requestmeilyn.garro@ucr.ac.crCosta RicaNo aplicaNo aplicaNo aplicaopendoar:18712025-03-29T18:06:36.583Kérwá - 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