Cultivable bacterial diversity along the altitudinal zonation and vegetation range of tropical Eastern Himalaya
The Northeastern part of India sprawls over an area of 262 379km2 in the Eastern Himalayan range. This constitutes a biodiversity hotspot with high levels of biodiversity and endemism; unfortunately, is also a poorly known area, especially on its microbial diversity. In this study, we assessed culti...
| Autores: | , , , |
|---|---|
| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2013 |
| País: | Costa Rica |
| Recursos: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| Idioma: | inglés |
| OAI Identifier: | oai:portal.ucr.ac.cr:article/11141 |
| Acesso em linha: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/11141 |
| Access Level: | acceso abierto |
| Palavra-chave: | cultivable bacteria eastern himalyan range tropical region altitude 16S rRNA diversity indices bacterias cultivables zona del este del himalaya región tropical altitud 16S rARN índices de diversidad |
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Cultivable bacterial diversity along the altitudinal zonation and vegetation range of tropical Eastern Himalaya Cultivable bacterial diversity along the altitudinal zonation and vegetation range of tropical Eastern Himalaya |
| title |
Cultivable bacterial diversity along the altitudinal zonation and vegetation range of tropical Eastern Himalaya |
| spellingShingle |
Cultivable bacterial diversity along the altitudinal zonation and vegetation range of tropical Eastern Himalaya Lyngwi, Nathaniel A. cultivable bacteria eastern himalyan range tropical region altitude 16S rRNA diversity indices bacterias cultivables zona del este del himalaya región tropical altitud 16S rARN índices de diversidad |
| title_short |
Cultivable bacterial diversity along the altitudinal zonation and vegetation range of tropical Eastern Himalaya |
| title_full |
Cultivable bacterial diversity along the altitudinal zonation and vegetation range of tropical Eastern Himalaya |
| title_fullStr |
Cultivable bacterial diversity along the altitudinal zonation and vegetation range of tropical Eastern Himalaya |
| title_full_unstemmed |
Cultivable bacterial diversity along the altitudinal zonation and vegetation range of tropical Eastern Himalaya |
| title_sort |
Cultivable bacterial diversity along the altitudinal zonation and vegetation range of tropical Eastern Himalaya |
| dc.creator.none.fl_str_mv |
Lyngwi, Nathaniel A. Koijam, Khedarani Sharma, D. Joshi, S. R. |
| author |
Lyngwi, Nathaniel A. |
| author_facet |
Lyngwi, Nathaniel A. Koijam, Khedarani Sharma, D. Joshi, S. R. |
| author_role |
author |
| author2 |
Koijam, Khedarani Sharma, D. Joshi, S. R. |
| author2_role |
author author author |
| dc.subject.none.fl_str_mv |
cultivable bacteria eastern himalyan range tropical region altitude 16S rRNA diversity indices bacterias cultivables zona del este del himalaya región tropical altitud 16S rARN índices de diversidad |
| topic |
cultivable bacteria eastern himalyan range tropical region altitude 16S rRNA diversity indices bacterias cultivables zona del este del himalaya región tropical altitud 16S rARN índices de diversidad |
| description |
The Northeastern part of India sprawls over an area of 262 379km2 in the Eastern Himalayan range. This constitutes a biodiversity hotspot with high levels of biodiversity and endemism; unfortunately, is also a poorly known area, especially on its microbial diversity. In this study, we assessed cultivable soil bacterial diversity and distribution from lowlands to highlands (34 to 3 990m.a.s.l.). Soil physico-chemical parameters and forest types across the different altitudes were characterized and correlated with bacterial distribution and diversity. Microbes from the soil samples were grown in Nutrient, Muller Hinton and Luria-Bertani agar plates and were initially characterized using biochemical methods. Parameters like dehydrogenase and urease activities, temperature, moisture content, pH, carbon content, bulk density of the sampled soil were measured for each site. Representative isolates were also subjected to 16S rDNA sequence analysis. A total of 155 cultivable bacterial isolates were characterized which were analyzed for richness, evenness and diversity indices. The tropical and sub-tropical forests supported higher bacterial diversity compared to temperate pine, temperate conifer, and sub-alpine rhododendron forests. The 16S rRNA phylogenetic analysis revealed that Firmicutes was the most common group followed by Proteobacteria and Bacteroidetes. Species belonging to the genera Bacillus and Pseudomonas were the most abundant. Bacterial CFU showed positive but insignificant correlation with soil parameters like pH (r=0.208), soil temperature (r=0.303), ambient temperature (r=0.443), soil carbon content (r=0.525), soil bulk density (r=0.268), soil urease (r=0.549) and soil dehydrogenase (r=0.492). Altitude (r=- 0.561) and soil moisture content (r=-0.051) showed negative correlation. Altitudinal gradient along with the vegetation and soil physico-chemical parameters were found to influence bacterial diversity and distribution. This study points out that this is a biome with a vast reservoir of bacteria which decrease with increasing altitudes, and highlights the microbiological importance of the poorly studied Eastern Himalayan range, justifying efforts to explore the prevalence of novel species in the biome. |
| publishDate |
2013 |
| dc.date.none.fl_str_mv |
2013-03-01 |
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info:eu-repo/semantics/publishedVersion Contribution artículo original http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article |
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article |
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publishedVersion |
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https://revistas.ucr.ac.cr/index.php/rbt/article/view/11141 10.15517/rbt.v61i1.11141 |
| url |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/11141 |
| identifier_str_mv |
10.15517/rbt.v61i1.11141 |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
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https://revistas.ucr.ac.cr/index.php/rbt/article/view/11141/10502 |
| dc.rights.none.fl_str_mv |
Derechos de autor 2013 Revista de Biología Tropical acceso abierto http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
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Derechos de autor 2013 Revista de Biología Tropical acceso abierto http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Universidad de Costa Rica |
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Universidad de Costa Rica |
| dc.source.none.fl_str_mv |
Revista de Biología Tropical; Vol. 61 No. 1 (2013): Volume 61 – Regular number 1 – March 2013; 467–490 Revista de Biología Tropical; Vol. 61 Núm. 1 (2013): Volumen 61 – Número regular 1 – Marzo 2013; 467–490 Revista Biología Tropical; Vol. 61 N.º 1 (2013): Volumen 61 – Número regular 1 – Marzo 2013; 467–490 2215-2075 0034-7744 10.15517/rbt.v61i1 reponame:Portal de Revistas UCR instname:Universidad de Costa Rica instacron:UCR |
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Universidad de Costa Rica |
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UCR |
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UCR |
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Portal de Revistas UCR |
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Portal de Revistas UCR |
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Portal de Revistas UCR - Universidad de Costa Rica |
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jorge.polanco@ucr.ac.cr |
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1849325356811550720 |
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Cultivable bacterial diversity along the altitudinal zonation and vegetation range of tropical Eastern HimalayaCultivable bacterial diversity along the altitudinal zonation and vegetation range of tropical Eastern HimalayaLyngwi, Nathaniel A.Koijam, KhedaraniSharma, D.Joshi, S. R.cultivable bacteriaeastern himalyan rangetropical regionaltitude16S rRNAdiversity indicesbacterias cultivableszona del este del himalayaregión tropicalaltitud16S rARNíndices de diversidadThe Northeastern part of India sprawls over an area of 262 379km2 in the Eastern Himalayan range. This constitutes a biodiversity hotspot with high levels of biodiversity and endemism; unfortunately, is also a poorly known area, especially on its microbial diversity. In this study, we assessed cultivable soil bacterial diversity and distribution from lowlands to highlands (34 to 3 990m.a.s.l.). Soil physico-chemical parameters and forest types across the different altitudes were characterized and correlated with bacterial distribution and diversity. Microbes from the soil samples were grown in Nutrient, Muller Hinton and Luria-Bertani agar plates and were initially characterized using biochemical methods. Parameters like dehydrogenase and urease activities, temperature, moisture content, pH, carbon content, bulk density of the sampled soil were measured for each site. Representative isolates were also subjected to 16S rDNA sequence analysis. A total of 155 cultivable bacterial isolates were characterized which were analyzed for richness, evenness and diversity indices. The tropical and sub-tropical forests supported higher bacterial diversity compared to temperate pine, temperate conifer, and sub-alpine rhododendron forests. The 16S rRNA phylogenetic analysis revealed that Firmicutes was the most common group followed by Proteobacteria and Bacteroidetes. Species belonging to the genera Bacillus and Pseudomonas were the most abundant. Bacterial CFU showed positive but insignificant correlation with soil parameters like pH (r=0.208), soil temperature (r=0.303), ambient temperature (r=0.443), soil carbon content (r=0.525), soil bulk density (r=0.268), soil urease (r=0.549) and soil dehydrogenase (r=0.492). Altitude (r=- 0.561) and soil moisture content (r=-0.051) showed negative correlation. Altitudinal gradient along with the vegetation and soil physico-chemical parameters were found to influence bacterial diversity and distribution. This study points out that this is a biome with a vast reservoir of bacteria which decrease with increasing altitudes, and highlights the microbiological importance of the poorly studied Eastern Himalayan range, justifying efforts to explore the prevalence of novel species in the biome.La parte noreste de la India se extiende sobre una superficie de 262 379km2 en la cordillera oriental del Himalaya. Es un punto de acceso con altos niveles de biodiversidad y endemismo; desafortunadamente, también es una zona poco conocida, sobre todo su diversidad microbiana. En este estudio se evaluó la diversidad de bacterias cultivables del suelo, su diversidad y distribución de las tierras bajas a las altas (34 a 3 990m.s.n.m). Se caracterizaron los parámetros físico-químicos del suelo y tipos de bosques a lo largo del gradiente altitudinal y se correlacionaron con la distribución y diversidad bacteriana. Los microbios del suelo se cultivaron en placas de agar enriquecido Muller Hinton y Luria-Bertani, e inicialmente se caracterizaron mediante métodos bioquímicos. Parámetros tales como actividad de la deshidrogenasa y ureasa, temperatura, contenido de humedad y de carbono, pH y densidad aparente del suelo se midieron en cada sitio. Aislamientos representativos también se sometieron al análisis secuencial de 16S rADN. Un total de 155 aislamientos bacterianos cultivables se caracterizaron para estimar los índices de riqueza, equidad y diversidad. Los bosques tropicales y subtropicales albergan una mayor diversidad bacteriana en comparación con los bosques templados de pino y coníferas, y los bosques subalpinos de rododendro. El análisis filogenético de 16S rARN reveló que Firmicutes fue el grupo más común, seguido de Proteobacteria y Bacteroidetes. Especies pertenecientes a los géneros Bacillus y Pseudomonas fueron las más abundantes. Las UFC bacterianas mostraron una positiva pero insignificante correlación con los parámetros del suelo, tales como pH (r=0.208), temperatura (r=0.303), temperatura ambiente (r=0.443), contenido de carbón (r=0.525), densidad aparente (r=0.268), ureasa (r=0.549) y deshidrogenasa (r=0.492). La altitud (r=-0.561) y el contenido de humedad del suelo (r=-0.051) mostraron una correlación negativa. Se encontró que el gradiente altitudinal, junto con la vegetación y los parámetros físico-químicos influyeron en la diversidad bacteriana y la distribución. Este estudio señala que este es un bioma con un vasto reservorio de bacterias que disminuyen con la altitud y pone en relieve la importancia microbiológica de la pobremente estudiada zona del este del Himalaya, lo que justifica los esfuerzos para explorar la prevalencia de nuevas especies en el bioma.Universidad de Costa Rica2013-03-01info:eu-repo/semantics/publishedVersionContributionartículo originalhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdfhttps://revistas.ucr.ac.cr/index.php/rbt/article/view/1114110.15517/rbt.v61i1.11141Revista de Biología Tropical; Vol. 61 No. 1 (2013): Volume 61 – Regular number 1 – March 2013; 467–490Revista de Biología Tropical; Vol. 61 Núm. 1 (2013): Volumen 61 – Número regular 1 – Marzo 2013; 467–490Revista Biología Tropical; Vol. 61 N.º 1 (2013): Volumen 61 – Número regular 1 – Marzo 2013; 467–4902215-20750034-774410.15517/rbt.v61i1reponame:Portal de Revistas UCRinstname:Universidad de Costa Ricainstacron:UCRenghttps://revistas.ucr.ac.cr/index.php/rbt/article/view/11141/10502Derechos de autor 2013 Revista de Biología Tropicalacceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccess2022-10-18T15:37:28Zoai:portal.ucr.ac.cr:article/11141Portal de revistashttps://revistas.ucr.ac.cr/Universidadhttp://www.ucr.ac.crhttps://revistas.ucr.ac.cr/index.php/index/oaijorge.polanco@ucr.ac.crCosta RicaNo aplicaNo aplicaNo aplicaopendoar:2025-08-13T10:15:22.367Portal de Revistas UCR - Universidad de Costa Ricafalse |
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15,812455 |