Biocontrol of Penicillium digitatum with secondary metabolites of endophytic fungi in mandarin fruits

Introduction. “Green mold,” caused by Penicillium digitatum is one of the phytosanitary issues that limits citrus production. For its control, synthetic fungicides are used, which have lost effectiveness due to resistance developed by the fungus. An alternative is the use of endophytic fungi with bi...

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Autores: León-Ttacca, Betsabe, Arestegui-Cantoral, Yasmin, Tarula-Gutierrez, Brandy, Orellana-Cornejo, Cesar, Luis-Vilcamiza, Jorge, Flores-Pelaez, Pedro
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:Costa Rica
Recursos:Universidad de Costa Rica
Repositorio:Portal de Revistas UCR
Idioma:español
inglés
OAI Identifier:oai:portal.ucr.ac.cr:article/55682
Acesso em linha:https://revistas.ucr.ac.cr/index.php/agromeso/article/view/55682
Access Level:acceso abierto
Palavra-chave:antibiosis
mold
biological control
antagonism
moho
control biológico
antagonismo
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dc.title.none.fl_str_mv Biocontrol of Penicillium digitatum with secondary metabolites of endophytic fungi in mandarin fruits
Biocontrol de Penicillium digitatum con metabolitos secundarios de hongos endófitos en frutos de mandarina
title Biocontrol of Penicillium digitatum with secondary metabolites of endophytic fungi in mandarin fruits
spellingShingle Biocontrol of Penicillium digitatum with secondary metabolites of endophytic fungi in mandarin fruits
León-Ttacca, Betsabe
antibiosis
mold
biological control
antagonism
moho
antibiosis
control biológico
antagonismo
title_short Biocontrol of Penicillium digitatum with secondary metabolites of endophytic fungi in mandarin fruits
title_full Biocontrol of Penicillium digitatum with secondary metabolites of endophytic fungi in mandarin fruits
title_fullStr Biocontrol of Penicillium digitatum with secondary metabolites of endophytic fungi in mandarin fruits
title_full_unstemmed Biocontrol of Penicillium digitatum with secondary metabolites of endophytic fungi in mandarin fruits
title_sort Biocontrol of Penicillium digitatum with secondary metabolites of endophytic fungi in mandarin fruits
dc.creator.none.fl_str_mv León-Ttacca, Betsabe
Arestegui-Cantoral, Yasmin
Tarula-Gutierrez, Brandy
Orellana-Cornejo, Cesar
Luis-Vilcamiza, Jorge
Flores-Pelaez, Pedro
author León-Ttacca, Betsabe
author_facet León-Ttacca, Betsabe
Arestegui-Cantoral, Yasmin
Tarula-Gutierrez, Brandy
Orellana-Cornejo, Cesar
Luis-Vilcamiza, Jorge
Flores-Pelaez, Pedro
author_role author
author2 Arestegui-Cantoral, Yasmin
Tarula-Gutierrez, Brandy
Orellana-Cornejo, Cesar
Luis-Vilcamiza, Jorge
Flores-Pelaez, Pedro
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv antibiosis
mold
biological control
antagonism
moho
antibiosis
control biológico
antagonismo
topic antibiosis
mold
biological control
antagonism
moho
antibiosis
control biológico
antagonismo
description Introduction. “Green mold,” caused by Penicillium digitatum is one of the phytosanitary issues that limits citrus production. For its control, synthetic fungicides are used, which have lost effectiveness due to resistance developed by the fungus. An alternative is the use of endophytic fungi with biocontrol action. Objective. To evaluate the effect of secondary metabolites (SM) from endophytic fungi (EF) on the mycelial inhibition of Penicillium digitatum and the effectiveness of disease control in mandarin fruits (Citrus reticulata) post-harvest. Materials and methods. The study was conducted in 2022, using a completely randomized experimental design in the Plant Health Laboratory at the Universidad Nacional de Cañete (UNDC), Lima, Peru. For the antibiosis test, secondary metabolites were extracted from ten strains of EF from the genera Trichoderma sp., Fusarium sp., and Aspergillus sp., where mycelial inhibition of the pathogen (%) was evaluated. SMs were sprayed at 15 % (v/v) concentration on mandarin fruits that were disinfected and inoculated with the pathogen by spraying (1x 105 CFU/cc). Additionally, a commercial biocontrol, a vegetable oil, and a fungicide were used. After 6 and 9 days, disease incidence (%) and severity index (0, 1, 2, 3) were evaluated. Results. Secondary metabolites from strains the HEA-111 (Aspergillus), HEA-109 (Fusarium), and HSA-1 (Trichoderma) showed the highest mycelial inhibition with 95.05 %, 87.51 %, and 47.47 %, respectively. In mandarin fruits treated with SMs from strains HEA-109 and HSA-1, there was a control of 80 % and 55 %, respectively. Conclusions. SMs from EF were effective in inhibiting the mycelial growth of P. digitatum and reduces disease progression in mandarin fruits post-harvest.  
publishDate 2024
dc.date.none.fl_str_mv 2024-04-11
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https://revistas.ucr.ac.cr/index.php/agromeso/article/view/55682/61175
https://revistas.ucr.ac.cr/index.php/agromeso/article/view/55682/62705
https://revistas.ucr.ac.cr/index.php/agromeso/article/view/55682/61177
https://revistas.ucr.ac.cr/index.php/agromeso/article/view/55682/61178
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dc.publisher.none.fl_str_mv Universidad de Costa Rica
publisher.none.fl_str_mv Universidad de Costa Rica
dc.source.none.fl_str_mv Agronomía Mesoamericana; Agronomia Mesoamericana: Vol. 35 ; 55682
Agronomía Mesoamericana; Agronomía Mesoamericana: Vol. 35 ; 55682
Agronomía Mesoamericana; Agronomía Mesoamericana: Vol. 35 ; 55682
2215-3608
1021-7444
reponame:Portal de Revistas UCR
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instname_str Universidad de Costa Rica
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reponame_str Portal de Revistas UCR
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repository.name.fl_str_mv Portal de Revistas UCR - Universidad de Costa Rica
repository.mail.fl_str_mv jorge.polanco@ucr.ac.cr
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spelling Biocontrol of Penicillium digitatum with secondary metabolites of endophytic fungi in mandarin fruitsBiocontrol de Penicillium digitatum con metabolitos secundarios de hongos endófitos en frutos de mandarinaLeón-Ttacca, BetsabeArestegui-Cantoral, YasminTarula-Gutierrez, BrandyOrellana-Cornejo, CesarLuis-Vilcamiza, JorgeFlores-Pelaez, Pedroantibiosismoldbiological controlantagonismmohoantibiosis control biológicoantagonismoIntroduction. “Green mold,” caused by Penicillium digitatum is one of the phytosanitary issues that limits citrus production. For its control, synthetic fungicides are used, which have lost effectiveness due to resistance developed by the fungus. An alternative is the use of endophytic fungi with biocontrol action. Objective. To evaluate the effect of secondary metabolites (SM) from endophytic fungi (EF) on the mycelial inhibition of Penicillium digitatum and the effectiveness of disease control in mandarin fruits (Citrus reticulata) post-harvest. Materials and methods. The study was conducted in 2022, using a completely randomized experimental design in the Plant Health Laboratory at the Universidad Nacional de Cañete (UNDC), Lima, Peru. For the antibiosis test, secondary metabolites were extracted from ten strains of EF from the genera Trichoderma sp., Fusarium sp., and Aspergillus sp., where mycelial inhibition of the pathogen (%) was evaluated. SMs were sprayed at 15 % (v/v) concentration on mandarin fruits that were disinfected and inoculated with the pathogen by spraying (1x 105 CFU/cc). Additionally, a commercial biocontrol, a vegetable oil, and a fungicide were used. After 6 and 9 days, disease incidence (%) and severity index (0, 1, 2, 3) were evaluated. Results. Secondary metabolites from strains the HEA-111 (Aspergillus), HEA-109 (Fusarium), and HSA-1 (Trichoderma) showed the highest mycelial inhibition with 95.05 %, 87.51 %, and 47.47 %, respectively. In mandarin fruits treated with SMs from strains HEA-109 and HSA-1, there was a control of 80 % and 55 %, respectively. Conclusions. SMs from EF were effective in inhibiting the mycelial growth of P. digitatum and reduces disease progression in mandarin fruits post-harvest.  Introducción. El “moho verde” causado por Penicillium digitatum es uno de los problemas fitosanitarios que limita la producción en los cítricos. Para su control se utilizan fungicidas sintéticos, que han perdido eficacia, debido a la resistencia generada por el hongo. Una alternativa, es el uso de hongos endófitos con acción biocontroladora. Objetivo. Evaluar el efecto de metabolitos secundarios (MS) de hongos endófitos (HE) en la inhibición micelial de Penicillium digitatum y la eficacia de control de la enfermedad, en frutos de mandarina (Citrus reticulata) después de la cosecha. Materiales y métodos. El estudio se realizó en 2022, bajo un diseño experimental completo al azar, en el laboratorio de Sanidad Vegetal, Universidad Nacional de Cañete (UNDC), Lima, Perú. Para la prueba de antibiosis se extrajeron los metabolitos secundarios de diez cepas de HE de los géneros Trichoderma sp., Fusarium sp. y Aspergillus sp., en donde se evaluó la inhibición micelial del patógeno (%). Se asperjaron MS a una concentración del 15 % (v/v) sobre frutos de mandarina que fueron desinfectados e inoculados con el patógeno por aspersión (1x105 UFC/cc). Adicionalmente, se utilizaron un biocontrol comercial, un aceite vegetal y un fungicida. Después de 6 y 9 días, se evaluaron la incidencia (%) y el índice de severidad (0,1,2,3) de la enfermedad. Resultados. Los metabolitos secundarios de las cepas HEA-111 (Aspergillus), HEA-109 (Fusarium) y HSA-1 (Trichoderma) presentaron la mayor inhibición micelial con 95,05 %, 87,51 % y 47,47 %, respectivamente. En los frutos de mandarina tratados con los MS de las cepas HEA-109 y HSA-1, hubo un control del 80 % y 55 %, respectivamente. Conclusiones. Los MS de HE tuvieron efecto en la inhibición micelial de P. digitatum y redujeron el avance de la enfermedad en frutos de mandarina después de la cosecha.Universidad de Costa Rica2024-04-11info:eu-repo/semantics/publishedVersionArticleTextTextoartículo originalhttp://purl.org/coar/resource_type/c_18cfhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articletext/xmlapplication/pdfapplication/epub+ziptext/htmlaudio/mpegaudio/mpeghttps://revistas.ucr.ac.cr/index.php/agromeso/article/view/5568210.15517/am.2024.55682Agronomía Mesoamericana; Agronomia Mesoamericana: Vol. 35 ; 55682 Agronomía Mesoamericana; Agronomía Mesoamericana: Vol. 35 ; 55682Agronomía Mesoamericana; Agronomía Mesoamericana: Vol. 35 ; 556822215-36081021-7444reponame:Portal de Revistas UCRinstname:Universidad de Costa Ricainstacron:UCRspaenghttps://revistas.ucr.ac.cr/index.php/agromeso/article/view/55682/62704https://revistas.ucr.ac.cr/index.php/agromeso/article/view/55682/59484https://revistas.ucr.ac.cr/index.php/agromeso/article/view/55682/61175https://revistas.ucr.ac.cr/index.php/agromeso/article/view/55682/62705https://revistas.ucr.ac.cr/index.php/agromeso/article/view/55682/61177https://revistas.ucr.ac.cr/index.php/agromeso/article/view/55682/61178Derechos de autor 2024 Betsabe León-Ttacca, Yasmin Arestegui-Cantoral, Brandy Tarula-Gutierrez, Cesar Orellana-Cornejo, Jorge Luis-Vilcamiza, Pedro Flores-Pelaeshttps://creativecommons.org/licenses/by-nc-nd/4.0acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccess2025-01-10T15:51:09Zoai:portal.ucr.ac.cr:article/55682Portal de revistashttps://revistas.ucr.ac.cr/Universidadhttp://www.ucr.ac.crhttps://revistas.ucr.ac.cr/index.php/index/oaijorge.polanco@ucr.ac.crCosta RicaNo aplicaNo aplicaNo aplicaopendoar:2025-08-13T10:30:10.105Portal de Revistas UCR - Universidad de Costa Ricafalse
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