In vivo neutralization of the dendrotoxin-mediated neurotoxicity of black mamba venom by oligoclonal human IgG monoclonal antibodies
The black mamba (Dendroaspis polylepis) is one of the most feared snake species of the African savanna. It has a potent, fast-acting neurotoxic venom comprised of dendrotoxins and α-neurotoxins associated with high fatality in untreated victims. Current antivenoms are both scarce on the African cont...
| Autores: | , , , , , , , , , , , , , , |
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| Tipo de documento: | artigo |
| Data de publicação: | 2018 |
| País: | Costa Rica |
| Recursos: | Universidad de Costa Rica |
| Repositório: | Kérwá |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/76386 |
| Acesso em linha: | https://www.nature.com/articles/s41467-018-06086-4 https://hdl.handle.net/10669/76386 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Serpientes Veneno África 615.946 Venenos animales |
| Resumo: | The black mamba (Dendroaspis polylepis) is one of the most feared snake species of the African savanna. It has a potent, fast-acting neurotoxic venom comprised of dendrotoxins and α-neurotoxins associated with high fatality in untreated victims. Current antivenoms are both scarce on the African continent and present a number of drawbacks as they are derived from the plasma of hyper-immunized large mammals. Here, we describe the development of an experimental recombinant antivenom by a combined toxicovenomics and phage display approach. The recombinant antivenom is based on a cocktail of fully human immunoglobulin G (IgG) monoclonal antibodies capable of neutralizing dendrotoxin-mediated neurotoxicity of black mamba whole venom in a rodent model. Our results show the potential use of fully human monoclonal IgGs against animal toxins and the first use of oligoclonal human IgG mixtures against experimental snakebite envenoming. |
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