Genetic analysis of tropical Beauveria and Metarhizium associated with sugar cane insects

Introduction. Entomopathogenic fungi are used as biopesticides to control invertebrate pests in agriculture. The generation of genetic information and its geographic relationships are important for diversity studies and for the protection of biological resources. Objective. The aim of this study was...

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Authors: Vargas-Martínez, Alejandro, Salazar-Blanco, José Daniel, González-Herrera, Allan, Molina Bravo, Ramón
Format: article
Status:Published version
Publication Date:2019
Country:Costa Rica
Institution:Universidad de Costa Rica
Repository:Portal de Revistas UCR
Language:Spanish
OAI Identifier:oai:portal.ucr.ac.cr:article/32307
Online Access:https://revistas.ucr.ac.cr/index.php/agromeso/article/view/32307
Access Level:Open access
Keyword:Coleoptera
entomopathogenic fungi
Hemiptera
microsatellites
Saccharum officinarum
Microsatélites
hongos entomopatógenos
id CR_895d2d457d0c8b6926b74c7440d2f63d
oai_identifier_str oai:portal.ucr.ac.cr:article/32307
network_acronym_str CR
network_name_str Costa Rica
repository_id_str
dc.title.none.fl_str_mv Genetic analysis of tropical Beauveria and Metarhizium associated with sugar cane insects
Análisis genético de Beauveria y Metarhizium tropicales asociados a insectos en caña de azúcar
title Genetic analysis of tropical Beauveria and Metarhizium associated with sugar cane insects
spellingShingle Genetic analysis of tropical Beauveria and Metarhizium associated with sugar cane insects
Vargas-Martínez, Alejandro
Coleoptera
entomopathogenic fungi
Hemiptera
microsatellites
Saccharum officinarum
Microsatélites
Coleoptera
Hemiptera
hongos entomopatógenos
Saccharum officinarum
title_short Genetic analysis of tropical Beauveria and Metarhizium associated with sugar cane insects
title_full Genetic analysis of tropical Beauveria and Metarhizium associated with sugar cane insects
title_fullStr Genetic analysis of tropical Beauveria and Metarhizium associated with sugar cane insects
title_full_unstemmed Genetic analysis of tropical Beauveria and Metarhizium associated with sugar cane insects
title_sort Genetic analysis of tropical Beauveria and Metarhizium associated with sugar cane insects
dc.creator.none.fl_str_mv Vargas-Martínez, Alejandro
Salazar-Blanco, José Daniel
González-Herrera, Allan
Molina Bravo, Ramón
author Vargas-Martínez, Alejandro
author_facet Vargas-Martínez, Alejandro
Salazar-Blanco, José Daniel
González-Herrera, Allan
Molina Bravo, Ramón
author_role author
author2 Salazar-Blanco, José Daniel
González-Herrera, Allan
Molina Bravo, Ramón
author2_role author
author
author
dc.subject.none.fl_str_mv Coleoptera
entomopathogenic fungi
Hemiptera
microsatellites
Saccharum officinarum
Microsatélites
Coleoptera
Hemiptera
hongos entomopatógenos
Saccharum officinarum
topic Coleoptera
entomopathogenic fungi
Hemiptera
microsatellites
Saccharum officinarum
Microsatélites
Coleoptera
Hemiptera
hongos entomopatógenos
Saccharum officinarum
description Introduction. Entomopathogenic fungi are used as biopesticides to control invertebrate pests in agriculture. The generation of genetic information and its geographic relationships are important for diversity studies and for the protection of biological resources. Objective. The aim of this study was to analyze the allelic diversity and the genetic and geographic relationships of several isolates of Beauveria and Metarhizium from Departamento de Investigación y Extensión de la Caña de Azúcar (DIECA) of Liga Agrícola Industrial de la Caña de Azúcar (LAICA). Materials and methods. This research was performed in DIECA and Universidad Nacional in 2014 and 2015. Fourteen and thirteen isolates of Beauveria and Metarhizium, respectively, were cultured from various Hymenoptera and Coleoptera insects of Costa Rica and Brazil. The isolates were analyzed using primer pairs that amplified simple sequence repeat (SSR) markers; twelve specific to Beauveria and thirteen specific to Metarhizium. Geographic and genetic correlation matrices were tested using a Mantel test. Results. All Beauveria primer pairs generated products, while only eight of the thirteen Metarhizium pairs generated products. Several sets of Beauveria primers were highly informative, but only one of the pairs was moderately informative in Metarhizium. Five Beauveria primer pairs generated unique genetic profiles in eight of the fourteen isolates (57%). Genetic relationships revealed two major clades among the Beauveria isolates, where one of these clades separated into two smaller groups. Metarhizium isolates were closely related, except for one (Mal12). A linear model explained 26% of the variability of the correlation between the genetic distances and the geographic distances found in Beauveria isolates, however, no relationships were found in Metarhizium isolates. Conclusion. A greater number of markers or different molecular marker technologies would be necessary to distinguish the isolates from the collection. The information in this work is useful for diversity studies and protection of intellectual property. Herein, we also consider search strategies to encounter diverse fungi.  
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
Article
artículo original
http://purl.org/coar/resource_type/c_2df8fbb1
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistas.ucr.ac.cr/index.php/agromeso/article/view/32307
10.15517/am.v30i1.32307
url https://revistas.ucr.ac.cr/index.php/agromeso/article/view/32307
identifier_str_mv 10.15517/am.v30i1.32307
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistas.ucr.ac.cr/index.php/agromeso/article/view/32307/36559
https://revistas.ucr.ac.cr/index.php/agromeso/article/view/32307/36574
https://revistas.ucr.ac.cr/index.php/agromeso/article/view/32307/36754
https://revistas.ucr.ac.cr/index.php/agromeso/article/view/32307/37012
dc.rights.none.fl_str_mv acceso abierto
http://purl.org/coar/access_right/c_abf2
info:eu-repo/semantics/openAccess
rights_invalid_str_mv acceso abierto
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
application/zip
text/xml
dc.publisher.none.fl_str_mv Universidad de Costa Rica
publisher.none.fl_str_mv Universidad de Costa Rica
dc.source.none.fl_str_mv Agronomía Mesoamericana; 2019: Agronomía Mesoamericana: Vol. 30, Issue 1 (January-April); 267-280
Agronomía Mesoamericana; 2019: Agronomía Mesoamericana: Vol. 30, Nº 1 (Enero-abril); 267-280
Agronomía Mesoamericana; 2019: Agronomía Mesoamericana: Vol. 30, Nº 1 (Enero-abril); 267-280
2215-3608
1021-7444
reponame:Portal de Revistas UCR
instname:Universidad de Costa Rica
instacron:UCR
instname_str Universidad de Costa Rica
instacron_str UCR
institution UCR
reponame_str Portal de Revistas UCR
collection Portal de Revistas UCR
repository.name.fl_str_mv Portal de Revistas UCR - Universidad de Costa Rica
repository.mail.fl_str_mv jorge.polanco@ucr.ac.cr
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spelling Genetic analysis of tropical Beauveria and Metarhizium associated with sugar cane insectsAnálisis genético de Beauveria y Metarhizium tropicales asociados a insectos en caña de azúcarVargas-Martínez, AlejandroSalazar-Blanco, José DanielGonzález-Herrera, AllanMolina Bravo, RamónColeopteraentomopathogenic fungiHemipteramicrosatellitesSaccharum officinarumMicrosatélitesColeopteraHemipterahongos entomopatógenosSaccharum officinarumIntroduction. Entomopathogenic fungi are used as biopesticides to control invertebrate pests in agriculture. The generation of genetic information and its geographic relationships are important for diversity studies and for the protection of biological resources. Objective. The aim of this study was to analyze the allelic diversity and the genetic and geographic relationships of several isolates of Beauveria and Metarhizium from Departamento de Investigación y Extensión de la Caña de Azúcar (DIECA) of Liga Agrícola Industrial de la Caña de Azúcar (LAICA). Materials and methods. This research was performed in DIECA and Universidad Nacional in 2014 and 2015. Fourteen and thirteen isolates of Beauveria and Metarhizium, respectively, were cultured from various Hymenoptera and Coleoptera insects of Costa Rica and Brazil. The isolates were analyzed using primer pairs that amplified simple sequence repeat (SSR) markers; twelve specific to Beauveria and thirteen specific to Metarhizium. Geographic and genetic correlation matrices were tested using a Mantel test. Results. All Beauveria primer pairs generated products, while only eight of the thirteen Metarhizium pairs generated products. Several sets of Beauveria primers were highly informative, but only one of the pairs was moderately informative in Metarhizium. Five Beauveria primer pairs generated unique genetic profiles in eight of the fourteen isolates (57%). Genetic relationships revealed two major clades among the Beauveria isolates, where one of these clades separated into two smaller groups. Metarhizium isolates were closely related, except for one (Mal12). A linear model explained 26% of the variability of the correlation between the genetic distances and the geographic distances found in Beauveria isolates, however, no relationships were found in Metarhizium isolates. Conclusion. A greater number of markers or different molecular marker technologies would be necessary to distinguish the isolates from the collection. The information in this work is useful for diversity studies and protection of intellectual property. Herein, we also consider search strategies to encounter diverse fungi.  Introducción. Los hongos entomopatógenos son importantes biopesticidas que se utilizan para controlar plagas invertebradas en la agricultura. La generación de información genética y sus relaciones geográficas son importantes para estudios de diversidad y para la protección de recursos biológicos. Objetivo. El objetivo de este trabajo fue analizar la diversidad alélica y las relaciones genéticas y geográficas de la colección de hongos entomopatógenos de los géneros Beauveria y Metarhizium del Departamento de Investigación y Extensión de la Caña de Azúcar (DIECA), de la Liga Agrícola Industrial de la Caña de Azúcar (LAICA). Materiales y métodos. El trabajo se llevó a cabo en DIECA y en la Universidad Nacional de Costa Rica, entre los años 2014 y 2015. Un total de catorce aislamientos de Beauveria y trece de Metarhizium fueron aislados de diversos himenópteros y coleópteros de Costa Rica y de Brasil. Se realizaron análisis de relaciones genéticas por medio de marcadores tipo microsatélite (SSR), doce para Beauveria y trece para Metarhizium. Se realizaron pruebas de Mantel para analizar las matrices geográficas y genéticas. Resultados. Todos los cebadores para amplificar SSRs en Beauveria generaron productos, mientras que solo ocho de los trece pares de Metarhizium generaron productos. Varios juegos de cebadores fueron altamente informativos para Beauveria. Solo uno de los pares de cebadores fue moderadamente informativo para los aislamientos de Metarhizium. Cinco pares de cebadores para SSR de Beauveria generaron perfiles genéticos únicos en ocho de los catorce aislamientos (57%). Las relaciones genéticas revelaron dos clados principales entre los aislamientos de Beauveria, donde uno de los clados se dividió en dos grupos. Los aislamientos de Metarhizium estuvieron relacionados estrechamente con la excepción de un aislamiento (MaI12). Un modelo lineal explicó el 26% de la variabilidad de la correlación entre las distancias genéticas y geográficas en los aislamientos de Beauveria. En Metarhizium no se encontró una correlación. Conclusión. Mayor número de marcadores u otro sistema de marcadores serían necesarios para distinguir los aislamientos de la colección, sin embargo, fueron altamente informativos y serán útiles para futuros estudios.Universidad de Costa Rica2019-01-01info:eu-repo/semantics/publishedVersionArticleartículo originalhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdftext/htmlapplication/ziptext/xmlhttps://revistas.ucr.ac.cr/index.php/agromeso/article/view/3230710.15517/am.v30i1.32307Agronomía Mesoamericana; 2019: Agronomía Mesoamericana: Vol. 30, Issue 1 (January-April); 267-280 Agronomía Mesoamericana; 2019: Agronomía Mesoamericana: Vol. 30, Nº 1 (Enero-abril); 267-280Agronomía Mesoamericana; 2019: Agronomía Mesoamericana: Vol. 30, Nº 1 (Enero-abril); 267-2802215-36081021-7444reponame:Portal de Revistas UCRinstname:Universidad de Costa Ricainstacron:UCRspahttps://revistas.ucr.ac.cr/index.php/agromeso/article/view/32307/36559https://revistas.ucr.ac.cr/index.php/agromeso/article/view/32307/36574https://revistas.ucr.ac.cr/index.php/agromeso/article/view/32307/36754https://revistas.ucr.ac.cr/index.php/agromeso/article/view/32307/37012acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccess2023-06-16T13:46:54Zoai:portal.ucr.ac.cr:article/32307Portal de revistashttps://revistas.ucr.ac.cr/Universidadhttp://www.ucr.ac.crhttps://revistas.ucr.ac.cr/index.php/index/oaijorge.polanco@ucr.ac.crCosta RicaNo aplicaNo aplicaNo aplicaopendoar:2025-08-13T10:29:32.798Portal de Revistas UCR - Universidad de Costa Ricafalse
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