Measurement of thyroid hormones and cortisol in horses with an automated immunoassay analyzer

Even though thyroid gland diseases are unusual in horses, it is important to have a reference interval for thyroid hormones in this species at every veterinary endocrine laboratory. In addition, cortisol and thyroid hormone measurement is useful in horses, not only for disease diagnosis, but as a to...

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Detalhes bibliográficos
Autores: Suárez-Esquivel, Marcela, Castro-Ramírez, Laura
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2016
País:Costa Rica
Recursos:Universidad Nacional de Costa Rica
Repositório:Portal de Revistas UNA
Idioma:inglês
espanhol
OAI Identifier:oai:www.revistas.una.ac.cr:article/8489
Acesso em linha:https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/8489
Access Level:Acceso aberto
Palavra-chave:horses
thyroid hormones
cortisol
reference interval
AIA-360®
equinos
hormonas tiroideas
intervalo de referencia
AIA-30®
id CR_868e3ad5dd263cb5670483f767e2fdc3
oai_identifier_str oai:www.revistas.una.ac.cr:article/8489
network_acronym_str CR
network_name_str Costa Rica
repository_id_str
dc.title.none.fl_str_mv Measurement of thyroid hormones and cortisol in horses with an automated immunoassay analyzer
Medición de hormonas tiroideas y cortisol en caballos mediante un analizador de inmunoensayo automatizado
title Measurement of thyroid hormones and cortisol in horses with an automated immunoassay analyzer
spellingShingle Measurement of thyroid hormones and cortisol in horses with an automated immunoassay analyzer
Suárez-Esquivel, Marcela
horses
thyroid hormones
cortisol
reference interval
AIA-360®
equinos
hormonas tiroideas
cortisol
intervalo de referencia
AIA-30®
title_short Measurement of thyroid hormones and cortisol in horses with an automated immunoassay analyzer
title_full Measurement of thyroid hormones and cortisol in horses with an automated immunoassay analyzer
title_fullStr Measurement of thyroid hormones and cortisol in horses with an automated immunoassay analyzer
title_full_unstemmed Measurement of thyroid hormones and cortisol in horses with an automated immunoassay analyzer
title_sort Measurement of thyroid hormones and cortisol in horses with an automated immunoassay analyzer
dc.creator.none.fl_str_mv Suárez-Esquivel, Marcela
Castro-Ramírez, Laura
author Suárez-Esquivel, Marcela
author_facet Suárez-Esquivel, Marcela
Castro-Ramírez, Laura
author_role author
author2 Castro-Ramírez, Laura
author2_role author
dc.subject.none.fl_str_mv horses
thyroid hormones
cortisol
reference interval
AIA-360®
equinos
hormonas tiroideas
cortisol
intervalo de referencia
AIA-30®
topic horses
thyroid hormones
cortisol
reference interval
AIA-360®
equinos
hormonas tiroideas
cortisol
intervalo de referencia
AIA-30®
description Even though thyroid gland diseases are unusual in horses, it is important to have a reference interval for thyroid hormones in this species at every veterinary endocrine laboratory. In addition, cortisol and thyroid hormone measurement is useful in horses, not only for disease diagnosis, but as a tool for monitoring and researching performance and metabolic rate.The objectives of this study included (i) to test the capacity of the automated analyzer AIA 360® (TOSOH Bioscience) to measure thyroid hormones and cortisol in equine serum in a reliable way, and (ii) to establish a reference interval for thyroid hormones in Costa Rican horses using the AIA-360®.A total of 68 healthy and treatment free horses (31 males and 37 females) were sampled. Horses were grouped into three different categories according to their ages. Serum concentrations of total thyroxine (TT4), free thyroxine (FT4), and cortisol were quantified with the AIA-360®. Statistic relationships of hormonal values, gender and age were determined using Kruskal-Wallis, Dunn and Wilcoxon (U Man-Whitney) tests. P <0.05 was considered significant.Selected reference intervals were: TT4 14.16 – 46.33 nmol/L, FT4 3.60 – 16.09 pmol/L and cortisol 23.45 – 166.64 nmol/L. No statistically significant relationship was found between gender, age, and hormone values. Besides, significant relationships (p<0.05) were found between hormone levels and some environmental parameters. Animals living at higher altitudes showed higher cortisol levels, while cortisol decreases as the environmental temperature rises. Moreover, the FT4 levels are higher as precipitation increases. Finally, TT4 seems to be lower as day light length increases; however, no statistical relationship was found (p<0.30).  Our results show that the use of the automated analyzer AIA-360® is suitable to measure thyroid hormones and cortisol, and that the values found are comparable with other studies and analysis techniques.
publishDate 2016
dc.date.none.fl_str_mv 2016-07-11
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
artículo original
http://purl.org/coar/resource_type/c_2df8fbb1
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/8489
10.15359/rcv.34-1.3
url https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/8489
identifier_str_mv 10.15359/rcv.34-1.3
dc.language.none.fl_str_mv eng
spa
language eng
spa
dc.relation.none.fl_str_mv https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/8489/9608
https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/8489/9706
dc.rights.none.fl_str_mv Derechos de autor 2016 Revista Ciencias Veterinarias
acceso abierto
http://purl.org/coar/access_right/c_abf2
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2016 Revista Ciencias Veterinarias
acceso abierto
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidad Nacional, Costa Rica
publisher.none.fl_str_mv Universidad Nacional, Costa Rica
dc.source.none.fl_str_mv Ciencias Veterinarias; Vol. 34 No. 1 (2016): Ciencias Veterinarias (Enero-Junio); 39-49
Ciencias veterinarias; Vol. 34 Núm. 1 (2016): Ciencias Veterinarias (Enero-Junio); 39-49
2215-4507
0250-5649
reponame:Portal de Revistas UNA
instname:Universidad Nacional de Costa Rica
instacron:UNA
instname_str Universidad Nacional de Costa Rica
instacron_str UNA
institution UNA
reponame_str Portal de Revistas UNA
collection Portal de Revistas UNA
repository.name.fl_str_mv Portal de Revistas UNA - Universidad Nacional de Costa Rica
repository.mail.fl_str_mv andrea.mora.campos@una.cr
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spelling Measurement of thyroid hormones and cortisol in horses with an automated immunoassay analyzerMedición de hormonas tiroideas y cortisol en caballos mediante un analizador de inmunoensayo automatizadoSuárez-Esquivel, MarcelaCastro-Ramírez, Laurahorsesthyroid hormonescortisolreference intervalAIA-360®equinoshormonas tiroideascortisolintervalo de referenciaAIA-30®Even though thyroid gland diseases are unusual in horses, it is important to have a reference interval for thyroid hormones in this species at every veterinary endocrine laboratory. In addition, cortisol and thyroid hormone measurement is useful in horses, not only for disease diagnosis, but as a tool for monitoring and researching performance and metabolic rate.The objectives of this study included (i) to test the capacity of the automated analyzer AIA 360® (TOSOH Bioscience) to measure thyroid hormones and cortisol in equine serum in a reliable way, and (ii) to establish a reference interval for thyroid hormones in Costa Rican horses using the AIA-360®.A total of 68 healthy and treatment free horses (31 males and 37 females) were sampled. Horses were grouped into three different categories according to their ages. Serum concentrations of total thyroxine (TT4), free thyroxine (FT4), and cortisol were quantified with the AIA-360®. Statistic relationships of hormonal values, gender and age were determined using Kruskal-Wallis, Dunn and Wilcoxon (U Man-Whitney) tests. P <0.05 was considered significant.Selected reference intervals were: TT4 14.16 – 46.33 nmol/L, FT4 3.60 – 16.09 pmol/L and cortisol 23.45 – 166.64 nmol/L. No statistically significant relationship was found between gender, age, and hormone values. Besides, significant relationships (p<0.05) were found between hormone levels and some environmental parameters. Animals living at higher altitudes showed higher cortisol levels, while cortisol decreases as the environmental temperature rises. Moreover, the FT4 levels are higher as precipitation increases. Finally, TT4 seems to be lower as day light length increases; however, no statistical relationship was found (p<0.30).  Our results show that the use of the automated analyzer AIA-360® is suitable to measure thyroid hormones and cortisol, and that the values found are comparable with other studies and analysis techniques.A pesar de que las enfermedades tiroideas son poco comunes en equinos, es importante contar con un intervalo de referencia para hormonas tiroideas en cualquier laboratorio de endocrinología veterinaria. Asimismo, la medición de cortisol y hormonas tiroideas es útil en caballos, no solamente en el diagnóstico de enfermedades, sino que, ambos tipos de hormonas, son utilizadas como herramientas para monitorización e investigación del desempeño físico y la tasa metabólica.Los objetivos de este estudio son: (i) probar la capacidad del analizador automatizado AIA 360® (TOSOH Bioscience) para la medición de hormonas tiroideas y cortisol en suero equino de forma confiable y; (ii) establecer un intervalo de referencia para hormonas tiroideas en caballos costarricenses utilizando el AIA -360®.Se muestreó 68 caballos saludables, libres de medicación (31 machos y 37 hembras) y se agruparon en tres categorías, con base en la edad. Las concentraciones séricas de tiroxina total (TT4), tiroxina libre (FT4) y cortisol fueron cuantificadas en el AIA-360®. La relación estadística, entre sexo, edad y valores hormonales, se determinó con las pruebas de Kruskal-Wallis, Dunn y Wilcoxon (U Man-Whitney). Se consideró significativo un valor de p <0.05.Los intervalos de referencia fueron los siguientes: TT4 14.16 – 46.33 nmol/L, FT4 3.60 – 16.09 pmol/L y cortisol 23.45 – 166.64 nmol/L. Estadísticamente, no se encontró relación significativa entre el sexo, la edad y los valores hormonales. Se observó relaciones significativas (p<0.05) entre los niveles hormonales y algunos de los parámetros medio ambientales en los cuales se encontraban los animales.  A mayor altitud, los niveles de cortisol fueron más elevados; mientras que, conforme aumentaba la temperatura ambiental, los valores de cortisol fueron más bajos. Los niveles de FT4 incrementaron a mayor precipitación. La concentración de TT4 tiende a disminuir conforme aumentan las horas-luz diarias. Sin embargo, no se encontró una relación estadística para ello (p<0.30). Los resultados muestran que el AIA-360® es útil para la medición de hormonas tiroideas y cortisol; además, que los valores encontrados son comparables con otros estudios y técnicas de análisis.Universidad Nacional, Costa Rica2016-07-11info:eu-repo/semantics/publishedVersionartículo originalhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdftext/htmlhttps://www.revistas.una.ac.cr/index.php/veterinaria/article/view/848910.15359/rcv.34-1.3Ciencias Veterinarias; Vol. 34 No. 1 (2016): Ciencias Veterinarias (Enero-Junio); 39-49Ciencias veterinarias; Vol. 34 Núm. 1 (2016): Ciencias Veterinarias (Enero-Junio); 39-492215-45070250-5649reponame:Portal de Revistas UNAinstname:Universidad Nacional de Costa Ricainstacron:UNAengspahttps://www.revistas.una.ac.cr/index.php/veterinaria/article/view/8489/9608https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/8489/9706Derechos de autor 2016 Revista Ciencias Veterinariasacceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccess2019-08-27T15:19:54Zoai:www.revistas.una.ac.cr:article/8489Portal de revistashttp://revistas.una.ac.cr/Universidadhttp://www.una.ac.crhttps://revistas.una.ac.cr/index.php/index/oaiandrea.mora.campos@una.crCosta RicaNo aplicaNo aplicaNo aplicaopendoar:2019-08-27T15:19:54Portal de Revistas UNA - Universidad Nacional de Costa Ricafalse
score 15.812455

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