Evaluation of the diagnosis of Ehrlichia canis in dogs from Costa Rica, using blood smears and molecular technique

The objective of this study was to compare the results of the diagnosis of Ehrlichia canis in blood of dogs from the Central Valley of Costa Rica, using blood smears and the polymerase chain reaction (PCR). For this purpose, 300 blood samples of dogs suspected of suffering ehrlichiosis were used. Sa...

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Autores: Romero Pérez, Luis Ernesto, Dolz Wiedner, Gaby, Romero Zúñiga, Juan José, Meneses Guevara, Ana, Jiménez Soto, Mauricio, Salazar Sánchez, Lizbeth
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Costa Rica
Institución:Universidad Nacional de Costa Rica
Repositorio:Portal de Revistas UNA
Idioma:español
OAI Identifier:oai:www.revistas.una.ac.cr:article/5425
Acceso en línea:https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/5425
Access Level:acceso abierto
Palabra clave:Ehrlichia canis
blood smear
PCR
dogs
Costa Rica
frotis sanguíneo
perros
id CR_84f9fa974eb9a5e0d4ea4900c1f2f70e
oai_identifier_str oai:www.revistas.una.ac.cr:article/5425
network_acronym_str CR
network_name_str Costa Rica
repository_id_str
dc.title.none.fl_str_mv Evaluation of the diagnosis of Ehrlichia canis in dogs from Costa Rica, using blood smears and molecular technique
Evaluación del diagnóstico de Ehrlichia canis mediante frotis sanguíneo y técnica molecular en perros de Costa Rica
title Evaluation of the diagnosis of Ehrlichia canis in dogs from Costa Rica, using blood smears and molecular technique
spellingShingle Evaluation of the diagnosis of Ehrlichia canis in dogs from Costa Rica, using blood smears and molecular technique
Romero Pérez, Luis Ernesto
Ehrlichia canis
blood smear
PCR
dogs
Costa Rica
Ehrlichia canis
frotis sanguíneo
PCR
perros
Costa Rica
title_short Evaluation of the diagnosis of Ehrlichia canis in dogs from Costa Rica, using blood smears and molecular technique
title_full Evaluation of the diagnosis of Ehrlichia canis in dogs from Costa Rica, using blood smears and molecular technique
title_fullStr Evaluation of the diagnosis of Ehrlichia canis in dogs from Costa Rica, using blood smears and molecular technique
title_full_unstemmed Evaluation of the diagnosis of Ehrlichia canis in dogs from Costa Rica, using blood smears and molecular technique
title_sort Evaluation of the diagnosis of Ehrlichia canis in dogs from Costa Rica, using blood smears and molecular technique
dc.creator.none.fl_str_mv Romero Pérez, Luis Ernesto
Dolz Wiedner, Gaby
Romero Zúñiga, Juan José
Meneses Guevara, Ana
Jiménez Soto, Mauricio
Salazar Sánchez, Lizbeth
author Romero Pérez, Luis Ernesto
author_facet Romero Pérez, Luis Ernesto
Dolz Wiedner, Gaby
Romero Zúñiga, Juan José
Meneses Guevara, Ana
Jiménez Soto, Mauricio
Salazar Sánchez, Lizbeth
author_role author
author2 Dolz Wiedner, Gaby
Romero Zúñiga, Juan José
Meneses Guevara, Ana
Jiménez Soto, Mauricio
Salazar Sánchez, Lizbeth
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ehrlichia canis
blood smear
PCR
dogs
Costa Rica
Ehrlichia canis
frotis sanguíneo
PCR
perros
Costa Rica
topic Ehrlichia canis
blood smear
PCR
dogs
Costa Rica
Ehrlichia canis
frotis sanguíneo
PCR
perros
Costa Rica
description The objective of this study was to compare the results of the diagnosis of Ehrlichia canis in blood of dogs from the Central Valley of Costa Rica, using blood smears and the polymerase chain reaction (PCR). For this purpose, 300 blood samples of dogs suspected of suffering ehrlichiosis were used. Samples were obtained from different veterinary clinics and had been previously analyzed using blood smears, nested PCR, and, in some cases, the serological technique. In addition, the following information was obtained from each blood sample: geographical location of the veterinary clinic and data from the dog such as race, age, sex, and clinical signs.Of the total 300 samples analyzed, 147 (49.0%) were positive for E. canis, using a nested PCR, while 178 (59.3%) were reported with inclusions using blood smears. However, only 103 (57.9%) of the positive cases using blood smear resulted in the molecular diagnosis of E. canis; while of the 122 negative samples (no inclusions) 44 (36.0%) were positive using the PCR technique. Blood smear testing showed a sensitivity of 70.1% and a specificity of 51.0%; positive and negative predictive values were 57.9% of 63.9%, respectively, compared with the PCR technique. The logistic regression analysis showed a positive association (p<0.05) with the presence of inclusions, but not for age or sex, while highly significant differences were detected using the Student’s T-Test in the average hematocrit and hemoglobin variables between PCR positive and PCR negative dogs. Of the 30 samples previously evaluated by serological tests, 25.0% of seropositive cases and 36.4% of seronegative cases were positive in the molecular diagnosis of E. canis. Low levels of hematocrit and hemoglobin, together with clinical signs and inclusions detected in blood samples, represent a strong indication of ehrlichiosis infection.However, a molecular technique is required to confirm the diagnosis.
publishDate 2013
dc.date.none.fl_str_mv 2013-11-20
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
artículo original
http://purl.org/coar/resource_type/c_2df8fbb1
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/5425
url https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/5425
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/5425/5257
dc.rights.none.fl_str_mv acceso abierto
http://purl.org/coar/access_right/c_abf2
info:eu-repo/semantics/openAccess
rights_invalid_str_mv acceso abierto
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional, Costa Rica
publisher.none.fl_str_mv Universidad Nacional, Costa Rica
dc.source.none.fl_str_mv Ciencias Veterinarias; Vol. 28 No. 1 (2010): Ciencias Veterinarias (Enero-Junio); 23-36
Ciencias veterinarias; Vol. 28 Núm. 1 (2010): Ciencias Veterinarias (Enero-Junio); 23-36
2215-4507
0250-5649
reponame:Portal de Revistas UNA
instname:Universidad Nacional de Costa Rica
instacron:UNA
instname_str Universidad Nacional de Costa Rica
instacron_str UNA
institution UNA
reponame_str Portal de Revistas UNA
collection Portal de Revistas UNA
repository.name.fl_str_mv Portal de Revistas UNA - Universidad Nacional de Costa Rica
repository.mail.fl_str_mv andrea.mora.campos@una.cr
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spelling Evaluation of the diagnosis of Ehrlichia canis in dogs from Costa Rica, using blood smears and molecular techniqueEvaluación del diagnóstico de Ehrlichia canis mediante frotis sanguíneo y técnica molecular en perros de Costa RicaRomero Pérez, Luis ErnestoDolz Wiedner, GabyRomero Zúñiga, Juan JoséMeneses Guevara, AnaJiménez Soto, MauricioSalazar Sánchez, LizbethEhrlichia canisblood smearPCRdogsCosta RicaEhrlichia canisfrotis sanguíneoPCRperrosCosta RicaThe objective of this study was to compare the results of the diagnosis of Ehrlichia canis in blood of dogs from the Central Valley of Costa Rica, using blood smears and the polymerase chain reaction (PCR). For this purpose, 300 blood samples of dogs suspected of suffering ehrlichiosis were used. Samples were obtained from different veterinary clinics and had been previously analyzed using blood smears, nested PCR, and, in some cases, the serological technique. In addition, the following information was obtained from each blood sample: geographical location of the veterinary clinic and data from the dog such as race, age, sex, and clinical signs.Of the total 300 samples analyzed, 147 (49.0%) were positive for E. canis, using a nested PCR, while 178 (59.3%) were reported with inclusions using blood smears. However, only 103 (57.9%) of the positive cases using blood smear resulted in the molecular diagnosis of E. canis; while of the 122 negative samples (no inclusions) 44 (36.0%) were positive using the PCR technique. Blood smear testing showed a sensitivity of 70.1% and a specificity of 51.0%; positive and negative predictive values were 57.9% of 63.9%, respectively, compared with the PCR technique. The logistic regression analysis showed a positive association (p<0.05) with the presence of inclusions, but not for age or sex, while highly significant differences were detected using the Student’s T-Test in the average hematocrit and hemoglobin variables between PCR positive and PCR negative dogs. Of the 30 samples previously evaluated by serological tests, 25.0% of seropositive cases and 36.4% of seronegative cases were positive in the molecular diagnosis of E. canis. Low levels of hematocrit and hemoglobin, together with clinical signs and inclusions detected in blood samples, represent a strong indication of ehrlichiosis infection.However, a molecular technique is required to confirm the diagnosis.El objetivo de este trabajo fue comparar los resultados del diagnóstico de Ehrlichia canis, mediante frotis sanguíneo y técnica de reacción en cadena de la polimerasa, (PCR, Polymerase Chain Reaction, por sus siglas en inglés) en sangre de perros del Valle Central de Costa Rica. Para este fin, se utilizaron 300 muestras sanguíneas de caninos sospechosos de sufrir ehrliquiosis; estas muestras habían sido remitidas de diferentes clínicas veterinarias; también, habían sido analizadas, previamente, mediante frotis sanguíneo, PCR anidado, y en algunos casos, mediante técnica serológica. De cada muestra de sangre se obtuvo, además, información sobre: localización geográfica de la clínica veterinaria, datos del perro al que pertenecía la muestra sanguínea como: raza, edad, sexo y signos clínicos que presentó el perro. Del total de 300 muestras analizadas, en 147 (49,0%) hubo resultados positivos a E. canis, utilizando un PCR anidado; mientras que, en 178 (59,3%) fueron reportadas con inclusiones mediante la técnica de frotis sanguíneo. Solamente, en 103 (57,9%) de los casos positivos, en frotis sanguíneo, fue posible el diagnóstico molecular de E. canis; mientras que de las 122 muestras reportadas negativas (sin inclusiones) un 36,0% (44) de los casos resultaron positivas en la técnica de PCR. Para la prueba de frotis sanguíneo se determinó una sensibilidad del 70,1% y una especificidad del 51,0%, valor predictivo positivo de 57,9% y valor predictivo negativo de 63,9%, comparado con la técnica de PCR. El análisis de regresión logística reveló asociación positiva (p<0,05) con la presencia de inclusiones, pero no con la edad o el sexo del animal; mientras que, la aplicación de la prueba t-student detectó diferencias altamente significativas en los promedios de las variables de hematocrito y hemoglobina entre perros PCR positivos y perros PCR negativos a E. canis. De las 30 muestras evaluadas, previamente, mediante serología, un 25,0% de los casos seropositivos y un 36,4% de los casos seronegativos resultaron positivos en el diagnóstico molecular de E. canis. El hallazgo de valores bajos de hematocrito y hemoglobina, en combinación con signos clínicos y la detección de inclusiones en muestras sanguíneas, representan un fuerte indicio de infección con ehrliquiosis; sin embargo, es necesaria la aplicación de una técnica molecular como método diagnóstico confirmativo.Universidad Nacional, Costa Rica2013-11-20info:eu-repo/semantics/publishedVersionartículo originalhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdfhttps://www.revistas.una.ac.cr/index.php/veterinaria/article/view/5425Ciencias Veterinarias; Vol. 28 No. 1 (2010): Ciencias Veterinarias (Enero-Junio); 23-36Ciencias veterinarias; Vol. 28 Núm. 1 (2010): Ciencias Veterinarias (Enero-Junio); 23-362215-45070250-5649reponame:Portal de Revistas UNAinstname:Universidad Nacional de Costa Ricainstacron:UNAspahttps://www.revistas.una.ac.cr/index.php/veterinaria/article/view/5425/5257acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccess2020-02-03T06:41:18Zoai:www.revistas.una.ac.cr:article/5425Portal de revistashttp://revistas.una.ac.cr/Universidadhttp://www.una.ac.crhttps://revistas.una.ac.cr/index.php/index/oaiandrea.mora.campos@una.crCosta RicaNo aplicaNo aplicaNo aplicaopendoar:2020-02-03T06:41:18Portal de Revistas UNA - Universidad Nacional de Costa Ricafalse
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