Assessment of counting chambers on boar sperm parameters analyzed by a CASA-Mot system

Introduction. Understanding the variability in sperm kinetic values through different chambers depths, shows the importance to create a standard for quality control methods in the artificial insemination (AI) industry. Objective. The work aimed was to evaluate the spermatozoa kinetic parameters base...

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Detalles Bibliográficos
Autores: Valverde, Anthony, Madrigal-Valverde, Mónica
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:Costa Rica
Institución:Universidad de Costa Rica
Repositorio:Portal de Revistas UCR
Idioma:español
inglés
OAI Identifier:oai:portal.ucr.ac.cr:article/34145
Acceso en línea:https://revistas.ucr.ac.cr/index.php/agromeso/article/view/34145
Access Level:acceso abierto
Palabra clave:semen
boar
spermatozoa
reproduction
verraco
espermatozoide
reproducción
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repository_id_str
dc.title.none.fl_str_mv Assessment of counting chambers on boar sperm parameters analyzed by a CASA-Mot system
Evaluación de cámaras de recuento sobre parámetros espermáticos de verracos analizados con un sistema CASA-Mot
title Assessment of counting chambers on boar sperm parameters analyzed by a CASA-Mot system
spellingShingle Assessment of counting chambers on boar sperm parameters analyzed by a CASA-Mot system
Valverde, Anthony
semen
boar
spermatozoa
reproduction
semen
verraco
espermatozoide
reproducción
title_short Assessment of counting chambers on boar sperm parameters analyzed by a CASA-Mot system
title_full Assessment of counting chambers on boar sperm parameters analyzed by a CASA-Mot system
title_fullStr Assessment of counting chambers on boar sperm parameters analyzed by a CASA-Mot system
title_full_unstemmed Assessment of counting chambers on boar sperm parameters analyzed by a CASA-Mot system
title_sort Assessment of counting chambers on boar sperm parameters analyzed by a CASA-Mot system
dc.creator.none.fl_str_mv Valverde, Anthony
Madrigal-Valverde, Mónica
author Valverde, Anthony
author_facet Valverde, Anthony
Madrigal-Valverde, Mónica
author_role author
author2 Madrigal-Valverde, Mónica
author2_role author
dc.subject.none.fl_str_mv semen
boar
spermatozoa
reproduction
semen
verraco
espermatozoide
reproducción
topic semen
boar
spermatozoa
reproduction
semen
verraco
espermatozoide
reproducción
description Introduction. Understanding the variability in sperm kinetic values through different chambers depths, shows the importance to create a standard for quality control methods in the artificial insemination (AI) industry. Objective. The work aimed was to evaluate the spermatozoa kinetic parameters based on different depths of the visualization chamber by means of a commercial system of computer-assisted sperm analysis, CASA-Mot. Materials and Methods. Twenty seminal doses of ten pietrain boars were used. The experimental period was from February to July 2017. The Integrated Semen Analyses System (ISAS®v1) with 50 Hz capture frequency was used. ISAS®D4C16 and ISAS®D4C20 counting chambers with a height of 16 and 20 μm respectively and pre-heated to 37 °C were employed. Results. Higher values (p<0.05) were found for all kinetic parameters when the height of the counting chamber was 20 μm. The zone effects within the counting chamber were constant between the two heights, and the variations observed in the kinetic parameters were due to a random effect of the boar. When analyzing the zone effect within the counting chamber, the first three fields of analysis showed higher curvilinear and rectilinear velocity (p<0.05) than the following fields, which is attributed to the presence of passive movement (drifting). Conclusion. The greater amplitude and volume capacity within the counting chamber (20 μm versus 16 μm), could promote the unrestricted movement of the cells, which would explain the increase in the kinetic values as the chamber height increased. Studies on the technical conditions of seminal analysis should be continued in order to standardize valuation methods with CASA systems. 
publishDate 2019
dc.date.none.fl_str_mv 2019-05-01
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
Article
artículo original
http://purl.org/coar/resource_type/c_2df8fbb1
info:eu-repo/semantics/article
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dc.identifier.none.fl_str_mv https://revistas.ucr.ac.cr/index.php/agromeso/article/view/34145
10.15517/am.v30i1.34145
url https://revistas.ucr.ac.cr/index.php/agromeso/article/view/34145
identifier_str_mv 10.15517/am.v30i1.34145
dc.language.none.fl_str_mv spa
eng
language spa
eng
dc.relation.none.fl_str_mv https://revistas.ucr.ac.cr/index.php/agromeso/article/view/34145/37663
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https://revistas.ucr.ac.cr/index.php/agromeso/article/view/34145/37665
https://revistas.ucr.ac.cr/index.php/agromeso/article/view/34145/38022
https://revistas.ucr.ac.cr/index.php/agromeso/article/view/34145/38024
https://revistas.ucr.ac.cr/index.php/agromeso/article/view/34145/38396
dc.rights.none.fl_str_mv acceso abierto
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dc.publisher.none.fl_str_mv Universidad de Costa Rica
publisher.none.fl_str_mv Universidad de Costa Rica
dc.source.none.fl_str_mv Agronomía Mesoamericana; 2019: Agronomía Mesoamericana: Vol. 30, Issue 2 (May-August); 447-458
Agronomía Mesoamericana; 2019: Agronomía Mesoamericana: Vol. 30, Nº 2 (Mayo-agosto); 447-458
Agronomía Mesoamericana; 2019: Agronomía Mesoamericana: Vol. 30, Nº 2 (Mayo-agosto); 447-458
2215-3608
1021-7444
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institution UCR
reponame_str Portal de Revistas UCR
collection Portal de Revistas UCR
repository.name.fl_str_mv Portal de Revistas UCR - Universidad de Costa Rica
repository.mail.fl_str_mv jorge.polanco@ucr.ac.cr
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spelling Assessment of counting chambers on boar sperm parameters analyzed by a CASA-Mot systemEvaluación de cámaras de recuento sobre parámetros espermáticos de verracos analizados con un sistema CASA-MotValverde, AnthonyMadrigal-Valverde, MónicasemenboarspermatozoareproductionsemenverracoespermatozoidereproducciónIntroduction. Understanding the variability in sperm kinetic values through different chambers depths, shows the importance to create a standard for quality control methods in the artificial insemination (AI) industry. Objective. The work aimed was to evaluate the spermatozoa kinetic parameters based on different depths of the visualization chamber by means of a commercial system of computer-assisted sperm analysis, CASA-Mot. Materials and Methods. Twenty seminal doses of ten pietrain boars were used. The experimental period was from February to July 2017. The Integrated Semen Analyses System (ISAS®v1) with 50 Hz capture frequency was used. ISAS®D4C16 and ISAS®D4C20 counting chambers with a height of 16 and 20 μm respectively and pre-heated to 37 °C were employed. Results. Higher values (p<0.05) were found for all kinetic parameters when the height of the counting chamber was 20 μm. The zone effects within the counting chamber were constant between the two heights, and the variations observed in the kinetic parameters were due to a random effect of the boar. When analyzing the zone effect within the counting chamber, the first three fields of analysis showed higher curvilinear and rectilinear velocity (p<0.05) than the following fields, which is attributed to the presence of passive movement (drifting). Conclusion. The greater amplitude and volume capacity within the counting chamber (20 μm versus 16 μm), could promote the unrestricted movement of the cells, which would explain the increase in the kinetic values as the chamber height increased. Studies on the technical conditions of seminal analysis should be continued in order to standardize valuation methods with CASA systems. Introducción. La comprensión de la variabilidad en los valores de la cinética de los espermatozoides, por medio de diferentes profundidades de cámaras, demuestra la importancia de crear un estándar para los métodos de control de calidad en la industria de la inseminación artificial (IA). Objetivo. El objetivo del presente trabajo fue evaluar los parámetros de cinética de los espermatozoides con base en diferentes profundidades de cámara de visualización mediante un sistema comercial de análisis de esperma asistidos por computadora, CASA-Mot. Materiales y Métodos. Se utilizaron veinte dosis seminales de diez verracos pietrain. El periodo experimental fue de febrero a julio del año 2017. Se utilizó el sistema integrado de análisis de semen (ISAS®v1) con una frecuencia de captura de 50 Hz. Se utilizaron cámaras de recuento ISAS®D4C16 e ISAS®D4C20 con una altura de 16 y 20 μm, respectivamente y precalentadas a 37 °C. Resultados. Se encontraron valores superiores (p<0,05) para todos los parámetros cinéticos cuando la altura de la cámara de recuento fue de 20 μm. El efecto de zona dentro de la cámara de recuento se mantuvo constante entre las dos alturas, y las variaciones observadas en los parámetros cinéticos se debieron a un efecto aleatorio del verraco. Al analizar el efecto de zona dentro de la cámara de recuento, los primeros tres campos de análisis mostraron mayor velocidad curvilínea y rectilínea (p<0,05) que los siguientes campos, lo que se atribuye a la presencia de movimiento pasivo (drifting). Conclusión. La mayor amplitud y capacidad de volumen dentro de la cámara de recuento (20 μm versus 16 μm), podría favorecer el desplazamiento sin restricciones de las células, lo que explicaría el incremento en los valores cinéticos conforme aumentó la altura de la cámara. Deben continuarse los estudios sobre las condiciones técnicas del análisis seminal para estandarizar los métodos de valoración con los sistemas CASA.Universidad de Costa Rica2019-05-01info:eu-repo/semantics/publishedVersionArticleartículo originalhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdfapplication/epub+ziptext/htmlaudio/mpegaudio/mpegtext/xmlhttps://revistas.ucr.ac.cr/index.php/agromeso/article/view/3414510.15517/am.v30i1.34145Agronomía Mesoamericana; 2019: Agronomía Mesoamericana: Vol. 30, Issue 2 (May-August); 447-458 Agronomía Mesoamericana; 2019: Agronomía Mesoamericana: Vol. 30, Nº 2 (Mayo-agosto); 447-458Agronomía Mesoamericana; 2019: Agronomía Mesoamericana: Vol. 30, Nº 2 (Mayo-agosto); 447-4582215-36081021-7444reponame:Portal de Revistas UCRinstname:Universidad de Costa Ricainstacron:UCRspaenghttps://revistas.ucr.ac.cr/index.php/agromeso/article/view/34145/37663https://revistas.ucr.ac.cr/index.php/agromeso/article/view/34145/37664https://revistas.ucr.ac.cr/index.php/agromeso/article/view/34145/37665https://revistas.ucr.ac.cr/index.php/agromeso/article/view/34145/38022https://revistas.ucr.ac.cr/index.php/agromeso/article/view/34145/38024https://revistas.ucr.ac.cr/index.php/agromeso/article/view/34145/38396acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccess2023-06-16T13:46:32Zoai:portal.ucr.ac.cr:article/34145Portal de revistashttps://revistas.ucr.ac.cr/Universidadhttp://www.ucr.ac.crhttps://revistas.ucr.ac.cr/index.php/index/oaijorge.polanco@ucr.ac.crCosta RicaNo aplicaNo aplicaNo aplicaopendoar:2025-08-13T10:29:35.794Portal de Revistas UCR - Universidad de Costa Ricafalse
score 15.812455

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