A novel seed baiting technique for the epiphytic orchid Rhynchostele cervantesii, a means to acquire mycorrhizal fungi from protocorms

We developed a new and novel seed baiting technique sowing mature seeds of the epiphyitic orchid Rhynchostele cervantesii under natural conditions, We introduced a sponge in each package that may serve as a reservoir for water retention to benefit germination; In three of 22 packets we found protoco...

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Bibliographic Details
Authors: Cruz-Higareda, Jesús Bernardo, Luna-Rosales, Bárbara Susana, Barba-Álvarez, Amadeo
Format: article
Status:Published version
Publication Date:2015
Country:Costa Rica
Institution:Universidad de Costa Rica
Repository:Portal de Revistas UCR
Language:Spanish
OAI Identifier:oai:portal.ucr.ac.cr:article/18525
Online Access:https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/18525
Access Level:Open access
Keyword:Orchid
Epiphytic
Seed
Baiting
Mycorrhiza
Rhynchostele
Description
Summary:We developed a new and novel seed baiting technique sowing mature seeds of the epiphyitic orchid Rhynchostele cervantesii under natural conditions, We introduced a sponge in each package that may serve as a reservoir for water retention to benefit germination; In three of 22 packets we found protocorms in an early stage of development, six of wich were of sufficient size to warrant fungal isolations; Nine strains were isolated in pure culture and were inoculated on seeds and protocorms under in vitro conditions.