Venomics of the Duvernoy's gland secretion of the false coral snake Rhinobothryum bovallii (Andersson, 1916) and assessment of venom lethality towards synapsid and diapsid animal models

The Duvernoy's gland secretory proteome of the false coral snake Rhinobothryum bovallii (Costa Rica), unveiled applying bottom-up venomics, comprises a handful of toxins belonging to only three protein families, three-finger toxin (3FTx), cysteine-rich secretory protein (CRISP), and snake venom...

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Detalhes bibliográficos
Autores: Calvete Chornet, Juan José, Bonilla Murillo, Fabián, Granados Martínez, Sofía, Sanz, Libia, Lomonte, Bruno, Sasa Marín, Mahmood
Formato: artículo
Fecha de publicación:2020
País:Costa Rica
Recursos:Universidad de Costa Rica
Repositorio:Kérwá
Idioma:inglés
OAI Identifier:oai:kerwa.ucr.ac.cr:10669/83139
Acesso em linha:https://www.sciencedirect.com/science/article/abs/pii/S1874391920302505?via%3Dihub
https://hdl.handle.net/10669/83139
Access Level:acceso embargado
Palavra-chave:Snake venomics
Rear-fanged snake
Rhinobothryum bovallii
Duvernoy's gland secretion
Toxicovenomics
Diapsid-specific venom toxin
Colubridae 3FTx
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network_acronym_str CR
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repository_id_str
dc.title.es_ES.fl_str_mv Venomics of the Duvernoy's gland secretion of the false coral snake Rhinobothryum bovallii (Andersson, 1916) and assessment of venom lethality towards synapsid and diapsid animal models
title Venomics of the Duvernoy's gland secretion of the false coral snake Rhinobothryum bovallii (Andersson, 1916) and assessment of venom lethality towards synapsid and diapsid animal models
spellingShingle Venomics of the Duvernoy's gland secretion of the false coral snake Rhinobothryum bovallii (Andersson, 1916) and assessment of venom lethality towards synapsid and diapsid animal models
Calvete Chornet, Juan José
Snake venomics
Rear-fanged snake
Rhinobothryum bovallii
Duvernoy's gland secretion
Toxicovenomics
Diapsid-specific venom toxin
Colubridae 3FTx
title_short Venomics of the Duvernoy's gland secretion of the false coral snake Rhinobothryum bovallii (Andersson, 1916) and assessment of venom lethality towards synapsid and diapsid animal models
title_full Venomics of the Duvernoy's gland secretion of the false coral snake Rhinobothryum bovallii (Andersson, 1916) and assessment of venom lethality towards synapsid and diapsid animal models
title_fullStr Venomics of the Duvernoy's gland secretion of the false coral snake Rhinobothryum bovallii (Andersson, 1916) and assessment of venom lethality towards synapsid and diapsid animal models
title_full_unstemmed Venomics of the Duvernoy's gland secretion of the false coral snake Rhinobothryum bovallii (Andersson, 1916) and assessment of venom lethality towards synapsid and diapsid animal models
title_sort Venomics of the Duvernoy's gland secretion of the false coral snake Rhinobothryum bovallii (Andersson, 1916) and assessment of venom lethality towards synapsid and diapsid animal models
dc.creator.none.fl_str_mv Calvete Chornet, Juan José
Bonilla Murillo, Fabián
Granados Martínez, Sofía
Sanz, Libia
Lomonte, Bruno
Sasa Marín, Mahmood
author Calvete Chornet, Juan José
author_facet Calvete Chornet, Juan José
Bonilla Murillo, Fabián
Granados Martínez, Sofía
Sanz, Libia
Lomonte, Bruno
Sasa Marín, Mahmood
author_role author
author2 Bonilla Murillo, Fabián
Granados Martínez, Sofía
Sanz, Libia
Lomonte, Bruno
Sasa Marín, Mahmood
author2_role author
author
author
author
author
dc.subject.es_ES.fl_str_mv Snake venomics
Rear-fanged snake
Rhinobothryum bovallii
Duvernoy's gland secretion
Toxicovenomics
Diapsid-specific venom toxin
Colubridae 3FTx
topic Snake venomics
Rear-fanged snake
Rhinobothryum bovallii
Duvernoy's gland secretion
Toxicovenomics
Diapsid-specific venom toxin
Colubridae 3FTx
description The Duvernoy's gland secretory proteome of the false coral snake Rhinobothryum bovallii (Costa Rica), unveiled applying bottom-up venomics, comprises a handful of toxins belonging to only three protein families, three-finger toxin (3FTx), cysteine-rich secretory protein (CRISP), and snake venom metalloprotease (PIII-SVMP). Except for small differences in the relative abundance of the PIII-SVMPs, which may be due to individual variability, no evidence of geographic variability or ontogenetic changes was found among the venom proteomes of the juvenile and adult R. bovallii snakes sampled. Major monomeric (86.5%mol) and minor dimeric (2.8%mol) 3FTxs dominate the toxin arsenal of the Costa Rican false coral snake. The remaining 10.7% of the venom proteome comprises CRISP (8.2%) and PIII-SVMP (2.4%) molecules. In vivo lethality assays showed that R. bovallii produces venom that is non-toxic towards mammalian prey, and which exerts a different toxic effect on domestic chicken chicks and baby green iguana. Toxicovenomic analysis of R. bovallii venom in the iguana model identified two 3FTx RP-HPLC fractions that faithfully mimicked the irreversible immobilizing effect of the whole venom.
publishDate 2020
dc.date.issued.none.fl_str_mv 2020
dc.date.accessioned.none.fl_str_mv 2021-03-24T15:07:19Z
dc.date.available.none.fl_str_mv 2021-03-24T15:07:19Z
dc.type.none.fl_str_mv artículo original
http://purl.org/coar/resource_type/c_2df8fbb1
info:eu-repo/semantics/article
format article
dc.identifier.citation.none.fl_str_mv https://www.sciencedirect.com/science/article/abs/pii/S1874391920302505?via%3Dihub
dc.identifier.issn.none.fl_str_mv 1874-3919
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10669/83139
dc.identifier.doi.none.fl_str_mv 10.1016/j.jprot.2020.103882
url https://www.sciencedirect.com/science/article/abs/pii/S1874391920302505?via%3Dihub
https://hdl.handle.net/10669/83139
identifier_str_mv 1874-3919
10.1016/j.jprot.2020.103882
dc.language.iso.es_ES.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv acceso embargado
http://purl.org/coar/access_right/c_f1cf
info:eu-repo/semantics/embargoedAccess
rights_invalid_str_mv acceso embargado
http://purl.org/coar/access_right/c_f1cf
eu_rights_str_mv embargoedAccess
dc.source.es_ES.fl_str_mv Journal of Proteomics, vol.225, pp.1-11
dc.source.none.fl_str_mv reponame:Kérwá
instname:Universidad de Costa Rica
instacron:UCR
instname_str Universidad de Costa Rica
instacron_str UCR
institution UCR
reponame_str Kérwá
collection Kérwá
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repository.name.fl_str_mv Kérwá - Universidad de Costa Rica
repository.mail.fl_str_mv meilyn.garro@ucr.ac.cr
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spelling Calvete Chornet, Juan José03718753-da20-4f77-b16a-f5867f73b0db600Bonilla Murillo, Fabián21c582ee-461d-4c78-8426-3dc1ed6b2781600Granados Martínez, Sofía21d8b813-3402-4a2b-99d0-a215f058ee0aSanz, Libiaa71fb767-584b-409f-b843-18c1977e6747600Lomonte, Bruno581484ad-aaa8-46b1-bc0e-f380d895640c600Sasa Marín, Mahmoode0a00694-ced7-4f73-85fc-cb7e5e4f96166002021-03-24T15:07:19Z2021-03-24T15:07:19Z2020https://www.sciencedirect.com/science/article/abs/pii/S1874391920302505?via%3Dihub1874-3919https://hdl.handle.net/10669/8313910.1016/j.jprot.2020.103882The Duvernoy's gland secretory proteome of the false coral snake Rhinobothryum bovallii (Costa Rica), unveiled applying bottom-up venomics, comprises a handful of toxins belonging to only three protein families, three-finger toxin (3FTx), cysteine-rich secretory protein (CRISP), and snake venom metalloprotease (PIII-SVMP). Except for small differences in the relative abundance of the PIII-SVMPs, which may be due to individual variability, no evidence of geographic variability or ontogenetic changes was found among the venom proteomes of the juvenile and adult R. bovallii snakes sampled. Major monomeric (86.5%mol) and minor dimeric (2.8%mol) 3FTxs dominate the toxin arsenal of the Costa Rican false coral snake. The remaining 10.7% of the venom proteome comprises CRISP (8.2%) and PIII-SVMP (2.4%) molecules. In vivo lethality assays showed that R. bovallii produces venom that is non-toxic towards mammalian prey, and which exerts a different toxic effect on domestic chicken chicks and baby green iguana. Toxicovenomic analysis of R. bovallii venom in the iguana model identified two 3FTx RP-HPLC fractions that faithfully mimicked the irreversible immobilizing effect of the whole venom.Ministerio de Ciencia, Innovación y Universidades/[BFU2017-89103-P]/MICIU/EspañaUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)UCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Biologíaengacceso embargadohttp://purl.org/coar/access_right/c_f1cfinfo:eu-repo/semantics/embargoedAccessJournal of Proteomics, vol.225, pp.1-11reponame:Kérwáinstname:Universidad de Costa Ricainstacron:UCRSnake venomicsRear-fanged snakeRhinobothryum bovalliiDuvernoy's gland secretionToxicovenomicsDiapsid-specific venom toxinColubridae 3FTxVenomics of the Duvernoy's gland secretion of the false coral snake Rhinobothryum bovallii (Andersson, 1916) and assessment of venom lethality towards synapsid and diapsid animal modelsartículo originalhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleORIGINAL2020_Prot_JProteomics_Calvete_Rhinobothryum_bovallii.pdf2020_Prot_JProteomics_Calvete_Rhinobothryum_bovallii.pdfVersión finalapplication/pdf1069935https://www.kerwa.ucr.ac.cr/bitstreams/e706eff5-97a0-4dda-98c9-407e6d8fb76e/download6870673f6ec8fbd1227b02f66b822723MD51LICENSElicense.txtlicense.txttext/plain; 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