Effects of different forms of nitrogen on rhizosphere microbial community structure of Eichhornia crassipes (Pontederiaceae)
Rhizosphere microbial communities are important for phytoremediation, plant nutrition, health and metabolism. Many factors, including plant species, pH and nutritional factors influence rhizosphere microbiology. In this study, we analysed the effects of different forms of nitrogen on the structures...
| Autores: | , |
|---|---|
| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2016 |
| País: | Costa Rica |
| Recursos: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| Idioma: | inglés |
| OAI Identifier: | oai:portal.ucr.ac.cr:article/18138 |
| Acesso em linha: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/18138 |
| Access Level: | acceso abierto |
| Palavra-chave: | Eichhornia crassipes nitrogen rhizosphere microbial community PCR-DGGE. nitrógeno comunidades microbianas de la rizósfera |
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oai:portal.ucr.ac.cr:article/18138 |
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Costa Rica |
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| dc.title.none.fl_str_mv |
Effects of different forms of nitrogen on rhizosphere microbial community structure of Eichhornia crassipes (Pontederiaceae) Efectos de diferentes formas de nitrógeno en la estructura de la rizósfera de comunidades microbianas de Eichhornia crassipes (Pontederiaceae) |
| title |
Effects of different forms of nitrogen on rhizosphere microbial community structure of Eichhornia crassipes (Pontederiaceae) |
| spellingShingle |
Effects of different forms of nitrogen on rhizosphere microbial community structure of Eichhornia crassipes (Pontederiaceae) Fu, Minghui Eichhornia crassipes nitrogen rhizosphere microbial community PCR-DGGE. Eichhornia crassipes nitrógeno comunidades microbianas de la rizósfera PCR-DGGE. |
| title_short |
Effects of different forms of nitrogen on rhizosphere microbial community structure of Eichhornia crassipes (Pontederiaceae) |
| title_full |
Effects of different forms of nitrogen on rhizosphere microbial community structure of Eichhornia crassipes (Pontederiaceae) |
| title_fullStr |
Effects of different forms of nitrogen on rhizosphere microbial community structure of Eichhornia crassipes (Pontederiaceae) |
| title_full_unstemmed |
Effects of different forms of nitrogen on rhizosphere microbial community structure of Eichhornia crassipes (Pontederiaceae) |
| title_sort |
Effects of different forms of nitrogen on rhizosphere microbial community structure of Eichhornia crassipes (Pontederiaceae) |
| dc.creator.none.fl_str_mv |
Fu, Minghui Zheng, Lijun |
| author |
Fu, Minghui |
| author_facet |
Fu, Minghui Zheng, Lijun |
| author_role |
author |
| author2 |
Zheng, Lijun |
| author2_role |
author |
| dc.subject.none.fl_str_mv |
Eichhornia crassipes nitrogen rhizosphere microbial community PCR-DGGE. Eichhornia crassipes nitrógeno comunidades microbianas de la rizósfera PCR-DGGE. |
| topic |
Eichhornia crassipes nitrogen rhizosphere microbial community PCR-DGGE. Eichhornia crassipes nitrógeno comunidades microbianas de la rizósfera PCR-DGGE. |
| description |
Rhizosphere microbial communities are important for phytoremediation, plant nutrition, health and metabolism. Many factors, including plant species, pH and nutritional factors influence rhizosphere microbiology. In this study, we analysed the effects of different forms of nitrogen on the structures of rhizosphere microbial communities of E. crassipes. Using a conventional culture method with special media, bacteria, actinobacteria and molds were cultured. We found that the numbers of bacteria were largely similar across the three culture conditions, while the numbers of actinobacteria and molds from the rhizosphere of E. crassipes cultured in NH4Cl solution were two orders of magnitude higher than those from the rhizospheres of plants cultured in distilled water and KNO3 solution. Using a culture-independent method of polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) of 16S rDNA, we found that the form of nitrogen could influence the components of the rhizosphere microbial community. Pseudoxanthomonas, Enterobacter and Citrobacter were present in all of the samples cultured under the three different experimental conditions. The genus Reyranella was found only in samples cultured in KNO3 solution; Acinetobacter and Streptomyces were unique to samples cultured in NH4Cl solution, and Pseudomonas, Pseudacidovorax and Methylosinus were found only in samples cultured in distilled water. Pseudoxanthomonas and Acidovorax were the dominant genera in the rhizosphere microbial community of E. crassipes cultured in KNO3 solution, while Novosphingobium was the dominant genus in the sample cultured in a nitrogen-deficient medium. Our results provide a theoretical foundation for using E. crassipes as a phytoremediation plant and controlling the widespread distribution of E. crassipes around the world using principles of nutrient metabolism. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016-03-01 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion Article artículo original http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/18138 10.15517/rbt.v64i1.18138 |
| url |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/18138 |
| identifier_str_mv |
10.15517/rbt.v64i1.18138 |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/18138/23008 https://revistas.ucr.ac.cr/index.php/rbt/article/view/18138/23009 |
| dc.rights.none.fl_str_mv |
Derechos de autor 2016 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 acceso abierto http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Derechos de autor 2016 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 acceso abierto http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf text/html |
| dc.publisher.none.fl_str_mv |
Universidad de Costa Rica |
| publisher.none.fl_str_mv |
Universidad de Costa Rica |
| dc.source.none.fl_str_mv |
Revista de Biología Tropical; Vol. 64 No. 1 (2016): Volume 64 – Regular number 1 – March 2016; 213–220 Revista de Biología Tropical; Vol. 64 Núm. 1 (2016): Volumen 64 – Número regular 1 – Marzo 2016; 213–220 Revista Biología Tropical; Vol. 64 N.º 1 (2016): Volumen 64 – Número regular 1 – Marzo 2016; 213–220 2215-2075 0034-7744 10.15517/rbt.v64i1 reponame:Portal de Revistas UCR instname:Universidad de Costa Rica instacron:UCR |
| instname_str |
Universidad de Costa Rica |
| instacron_str |
UCR |
| institution |
UCR |
| reponame_str |
Portal de Revistas UCR |
| collection |
Portal de Revistas UCR |
| repository.name.fl_str_mv |
Portal de Revistas UCR - Universidad de Costa Rica |
| repository.mail.fl_str_mv |
jorge.polanco@ucr.ac.cr |
| _version_ |
1849325364165214208 |
| spelling |
Effects of different forms of nitrogen on rhizosphere microbial community structure of Eichhornia crassipes (Pontederiaceae)Efectos de diferentes formas de nitrógeno en la estructura de la rizósfera de comunidades microbianas de Eichhornia crassipes (Pontederiaceae)Fu, MinghuiZheng, LijunEichhornia crassipesnitrogenrhizosphere microbial communityPCR-DGGE.Eichhornia crassipesnitrógenocomunidades microbianas de la rizósferaPCR-DGGE.Rhizosphere microbial communities are important for phytoremediation, plant nutrition, health and metabolism. Many factors, including plant species, pH and nutritional factors influence rhizosphere microbiology. In this study, we analysed the effects of different forms of nitrogen on the structures of rhizosphere microbial communities of E. crassipes. Using a conventional culture method with special media, bacteria, actinobacteria and molds were cultured. We found that the numbers of bacteria were largely similar across the three culture conditions, while the numbers of actinobacteria and molds from the rhizosphere of E. crassipes cultured in NH4Cl solution were two orders of magnitude higher than those from the rhizospheres of plants cultured in distilled water and KNO3 solution. Using a culture-independent method of polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) of 16S rDNA, we found that the form of nitrogen could influence the components of the rhizosphere microbial community. Pseudoxanthomonas, Enterobacter and Citrobacter were present in all of the samples cultured under the three different experimental conditions. The genus Reyranella was found only in samples cultured in KNO3 solution; Acinetobacter and Streptomyces were unique to samples cultured in NH4Cl solution, and Pseudomonas, Pseudacidovorax and Methylosinus were found only in samples cultured in distilled water. Pseudoxanthomonas and Acidovorax were the dominant genera in the rhizosphere microbial community of E. crassipes cultured in KNO3 solution, while Novosphingobium was the dominant genus in the sample cultured in a nitrogen-deficient medium. Our results provide a theoretical foundation for using E. crassipes as a phytoremediation plant and controlling the widespread distribution of E. crassipes around the world using principles of nutrient metabolism.Comunidades microbianas de la rizósfera son importantes para la fitorremediación, nutrición vegetal, salud y metabolismo. Muchos factores, incluyendo la especie de planta, el pH y los factores nutricionales influyen en la microbiología de la rizósfera. En este estudio, se analizaron los efectos de las diferentes formas del nitrógeno en la estructura de las comunidades microbianas de la rizósfera de E. crassipes. Mediante métodos de cultivo convencional con medios especiales se cultivaron: bacterias, actinobacterias y mohos. Se encontró que el número de bacterias era en gran parte similar a través de las tres condiciones de cultivo, mientras que el número de actinobacterias y mohos de la rizósfera de E. crassipes cultivadas en solución de NH4Cl era dos órdenes de magnitud superior a los de las rizósferas de plantas cultivadas en agua destilada y solución de KNO3. Utilizando un método de cultivo independiente de electroforesis en gel con gradiente de desnaturalización (PCR-DGGE) del ADNr 16S, se encontró que la forma de nitrógeno podría influir en los componentes de la comunidad microbiana de la rizósfera. Pseudoxanthomonas, Enterobacter y Citrobacter estaban presentes en todas las muestras cultivadas en las tres condiciones experimentales. El género Reyranella se encontró sólo en muestras cultivadas en solución de KNO3; Acinetobacter y Streptomyces eran las únicas muestras cultivadas en solución de NH4Cl, y Pseudomonas, Pseudacidovorax y Methylosinus se encontraron sólo en muestras cultivadas en agua destilada. Pseudoxanthomonas y Acidovorax eran los géneros dominantes en la comunidad microbiana de la rizósfera de E. crassipes cultivadas en solución de KNO3, mientras que Novos phingobium fue el género dominante en la muestra cultivada en un medio deficiente de nitrógeno. Nuestros resultados proporcionan una base teórica para el uso de E. crassipes como planta fitorremediadora y para controlar la distribución generalizada de E. crassipes en todo el mundo a través de los principios del metabolismo de nutrientes.Universidad de Costa Rica2016-03-01info:eu-repo/semantics/publishedVersionArticleartículo originalhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdftext/htmlhttps://revistas.ucr.ac.cr/index.php/rbt/article/view/1813810.15517/rbt.v64i1.18138Revista de Biología Tropical; Vol. 64 No. 1 (2016): Volume 64 – Regular number 1 – March 2016; 213–220Revista de Biología Tropical; Vol. 64 Núm. 1 (2016): Volumen 64 – Número regular 1 – Marzo 2016; 213–220Revista Biología Tropical; Vol. 64 N.º 1 (2016): Volumen 64 – Número regular 1 – Marzo 2016; 213–2202215-20750034-774410.15517/rbt.v64i1reponame:Portal de Revistas UCRinstname:Universidad de Costa Ricainstacron:UCRenghttps://revistas.ucr.ac.cr/index.php/rbt/article/view/18138/23008https://revistas.ucr.ac.cr/index.php/rbt/article/view/18138/23009Derechos de autor 2016 Revista de Biología Tropicalhttp://creativecommons.org/licenses/by/4.0acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccess2022-06-09T17:52:45Zoai:portal.ucr.ac.cr:article/18138Portal de revistashttps://revistas.ucr.ac.cr/Universidadhttp://www.ucr.ac.crhttps://revistas.ucr.ac.cr/index.php/index/oaijorge.polanco@ucr.ac.crCosta RicaNo aplicaNo aplicaNo aplicaopendoar:2025-08-13T10:17:54.782Portal de Revistas UCR - Universidad de Costa Ricafalse |
| score |
15,812455 |