Isolation of Fungi and Bacteria Associated with the Guts of Tropical Wood-Feeding Coleoptera and Determination of Their Lignocellulolytic Activities

The guts of beetle larvae constitute a complex system where relationships among fungi, bacteria, and the insect host occur. In this study, we collected larvae of five families of wood-feeding Coleoptera in tropical forests of Costa Rica, isolated fungi and bacteria from their intestinal tracts, and...

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Authors: Rojas Jiménez, Keilor Osvaldo, Hernández Alfaro, Myriam
Format: article
Publication Date:2015
Country:Costa Rica
Institution:Universidad de Costa Rica
Repository:Kérwá
OAI Identifier:oai:kerwa.ucr.ac.cr:10669/76308
Online Access:https://www.hindawi.com/journals/ijmicro/2015/285018/
https://hdl.handle.net/10669/76308
Access Level:Open access
Keyword:Microorganisms
Coleoptera
Lignocellulolytic Activities
599.472 867 Chiroptera (Quirópteros, Murciélagos)
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oai_identifier_str oai:kerwa.ucr.ac.cr:10669/76308
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network_name_str Costa Rica
repository_id_str
dc.title.es_ES.fl_str_mv Isolation of Fungi and Bacteria Associated with the Guts of Tropical Wood-Feeding Coleoptera and Determination of Their Lignocellulolytic Activities
title Isolation of Fungi and Bacteria Associated with the Guts of Tropical Wood-Feeding Coleoptera and Determination of Their Lignocellulolytic Activities
spellingShingle Isolation of Fungi and Bacteria Associated with the Guts of Tropical Wood-Feeding Coleoptera and Determination of Their Lignocellulolytic Activities
Rojas Jiménez, Keilor Osvaldo
Microorganisms
Coleoptera
Lignocellulolytic Activities
599.472 867 Chiroptera (Quirópteros, Murciélagos)
title_short Isolation of Fungi and Bacteria Associated with the Guts of Tropical Wood-Feeding Coleoptera and Determination of Their Lignocellulolytic Activities
title_full Isolation of Fungi and Bacteria Associated with the Guts of Tropical Wood-Feeding Coleoptera and Determination of Their Lignocellulolytic Activities
title_fullStr Isolation of Fungi and Bacteria Associated with the Guts of Tropical Wood-Feeding Coleoptera and Determination of Their Lignocellulolytic Activities
title_full_unstemmed Isolation of Fungi and Bacteria Associated with the Guts of Tropical Wood-Feeding Coleoptera and Determination of Their Lignocellulolytic Activities
title_sort Isolation of Fungi and Bacteria Associated with the Guts of Tropical Wood-Feeding Coleoptera and Determination of Their Lignocellulolytic Activities
dc.creator.none.fl_str_mv Rojas Jiménez, Keilor Osvaldo
Hernández Alfaro, Myriam
author Rojas Jiménez, Keilor Osvaldo
author_facet Rojas Jiménez, Keilor Osvaldo
Hernández Alfaro, Myriam
author_role author
author2 Hernández Alfaro, Myriam
author2_role author
dc.subject.es_ES.fl_str_mv Microorganisms
Coleoptera
Lignocellulolytic Activities
599.472 867 Chiroptera (Quirópteros, Murciélagos)
topic Microorganisms
Coleoptera
Lignocellulolytic Activities
599.472 867 Chiroptera (Quirópteros, Murciélagos)
description The guts of beetle larvae constitute a complex system where relationships among fungi, bacteria, and the insect host occur. In this study, we collected larvae of five families of wood-feeding Coleoptera in tropical forests of Costa Rica, isolated fungi and bacteria from their intestinal tracts, and determined the presence of five different pathways for lignocellulolytic activity. The fungal isolates were assigned to three phyla, 16 orders, 24 families, and 40 genera; Trichoderma was the most abundant genus, detected in all insect families and at all sites. The bacterial isolates were assigned to five phyla, 13 orders, 22 families, and 35 genera; Bacillus, Serratia, and Pseudomonas were the dominant genera, present in all the Coleopteran families. Positive results for activities related to degradation of wood components were determined in 65% and 48% of the fungal and bacterial genera, respectively. Our results showed that both the fungal and bacterial populations were highly diverse in terms of number of species and their phylogenetic composition, although the structure of the microbial communities varied with insect host family and the surrounding environment. The recurrent identification of some lignocellulolytic-positive inhabitants suggests that particular microbial groups play important roles in providing nutritional needs for the Coleopteran host.
publishDate 2015
dc.date.issued.none.fl_str_mv 2015-08
dc.date.accessioned.none.fl_str_mv 2018-12-12T19:45:44Z
dc.date.available.none.fl_str_mv 2018-12-12T19:45:44Z
dc.type.none.fl_str_mv artículo original
http://purl.org/coar/resource_type/c_2df8fbb1
info:eu-repo/semantics/article
format article
dc.identifier.citation.none.fl_str_mv https://www.hindawi.com/journals/ijmicro/2015/285018/
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10669/76308
dc.identifier.doi.none.fl_str_mv 10.1155/2015/285018
url https://www.hindawi.com/journals/ijmicro/2015/285018/
https://hdl.handle.net/10669/76308
identifier_str_mv 10.1155/2015/285018
dc.language.iso.es_ES.fl_str_mv en_US
language_invalid_str_mv en_US
dc.rights.none.fl_str_mv acceso abierto
http://purl.org/coar/access_right/c_abf2
info:eu-repo/semantics/openAccess
rights_invalid_str_mv acceso abierto
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.source.es_ES.fl_str_mv International Journal of Microbiology, vol. 2015, pp. 1-11
dc.source.none.fl_str_mv reponame:Kérwá
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instacron_str UCR
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spelling Rojas Jiménez, Keilor Osvaldobf62bc72-93fc-499d-8c27-a59ae6d3f1c2600Hernández Alfaro, Myriameeb7b45c-6eaa-423f-a1e3-b12f3992d7112018-12-12T19:45:44Z2018-12-12T19:45:44Z2015-08https://www.hindawi.com/journals/ijmicro/2015/285018/https://hdl.handle.net/10669/7630810.1155/2015/285018The guts of beetle larvae constitute a complex system where relationships among fungi, bacteria, and the insect host occur. In this study, we collected larvae of five families of wood-feeding Coleoptera in tropical forests of Costa Rica, isolated fungi and bacteria from their intestinal tracts, and determined the presence of five different pathways for lignocellulolytic activity. The fungal isolates were assigned to three phyla, 16 orders, 24 families, and 40 genera; Trichoderma was the most abundant genus, detected in all insect families and at all sites. The bacterial isolates were assigned to five phyla, 13 orders, 22 families, and 35 genera; Bacillus, Serratia, and Pseudomonas were the dominant genera, present in all the Coleopteran families. Positive results for activities related to degradation of wood components were determined in 65% and 48% of the fungal and bacterial genera, respectively. Our results showed that both the fungal and bacterial populations were highly diverse in terms of number of species and their phylogenetic composition, although the structure of the microbial communities varied with insect host family and the surrounding environment. The recurrent identification of some lignocellulolytic-positive inhabitants suggests that particular microbial groups play important roles in providing nutritional needs for the Coleopteran host.Consejo Nacional para Investigaciones Científicas y Tecnológicas/[FV-027-2007]/CONICIT/Costa RicaFundación CRUSA/[2007 CR0034]/CRUSA/Costa RicaUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Biologíaen_USacceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessInternational Journal of Microbiology, vol. 2015, pp. 1-11reponame:Kérwáinstname:Universidad de Costa Ricainstacron:UCRMicroorganismsColeopteraLignocellulolytic Activities599.472 867 Chiroptera (Quirópteros, Murciélagos)Isolation of Fungi and Bacteria Associated with the Guts of Tropical Wood-Feeding Coleoptera and Determination of Their Lignocellulolytic Activitiesartículo originalhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleORIGINAL2015-Rojas-IntJMicrob-Ligno.pdf2015-Rojas-IntJMicrob-Ligno.pdfVersión finalapplication/pdf252806https://www.kerwa.ucr.ac.cr/bitstreams/4db907cb-9fca-47c3-b86f-003991089909/download42d43228bbb631784bd0789a962b44aeMD51LICENSElicense.txtlicense.txttext/plain; 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