Mycorrhizal association in gametophytes and sporophytes of the fern Pteris vittata (Pteridaceae) with Glomus intraradices
Ferns, which are usually colonizing different environments and their roots frequently present mycorrhization, have two adult stages in their life cycle, the sporophytic and the gametophytic phase. This paper describes the experimental mycorrhizal association between Pteris vittata leptosporangiate f...
| Autores: | , , , , |
|---|---|
| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2012 |
| País: | Costa Rica |
| Recursos: | Universidad de Costa Rica |
| Repositório: | Portal de Revistas UCR |
| Idioma: | inglês |
| OAI Identifier: | oai:portal.ucr.ac.cr:article/4019 |
| Acesso em linha: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/4019 |
| Access Level: | Acceso aberto |
| Palavra-chave: | teris vittata glomus intraradices pteridophyta-gametophyte-sporophyte arum colonization paris colonization pteris vittata pteridophyta-gametofito esporofito colonización tipo arum colonización tipo paris |
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Mycorrhizal association in gametophytes and sporophytes of the fern Pteris vittata (Pteridaceae) with Glomus intraradices Mycorrhizal association in gametophytes and sporophytes of the fern Pteris vittata (Pteridaceae) with Glomus intraradices |
| title |
Mycorrhizal association in gametophytes and sporophytes of the fern Pteris vittata (Pteridaceae) with Glomus intraradices |
| spellingShingle |
Mycorrhizal association in gametophytes and sporophytes of the fern Pteris vittata (Pteridaceae) with Glomus intraradices Martinez, Alicia E. teris vittata glomus intraradices pteridophyta-gametophyte-sporophyte arum colonization paris colonization pteris vittata glomus intraradices pteridophyta-gametofito esporofito colonización tipo arum colonización tipo paris |
| title_short |
Mycorrhizal association in gametophytes and sporophytes of the fern Pteris vittata (Pteridaceae) with Glomus intraradices |
| title_full |
Mycorrhizal association in gametophytes and sporophytes of the fern Pteris vittata (Pteridaceae) with Glomus intraradices |
| title_fullStr |
Mycorrhizal association in gametophytes and sporophytes of the fern Pteris vittata (Pteridaceae) with Glomus intraradices |
| title_full_unstemmed |
Mycorrhizal association in gametophytes and sporophytes of the fern Pteris vittata (Pteridaceae) with Glomus intraradices |
| title_sort |
Mycorrhizal association in gametophytes and sporophytes of the fern Pteris vittata (Pteridaceae) with Glomus intraradices |
| dc.creator.none.fl_str_mv |
Martinez, Alicia E. Chiocchio, Viviana Em, Lo Tai Rodriguez, María A. Godeas, Alicia M. |
| author |
Martinez, Alicia E. |
| author_facet |
Martinez, Alicia E. Chiocchio, Viviana Em, Lo Tai Rodriguez, María A. Godeas, Alicia M. |
| author_role |
author |
| author2 |
Chiocchio, Viviana Em, Lo Tai Rodriguez, María A. Godeas, Alicia M. |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
teris vittata glomus intraradices pteridophyta-gametophyte-sporophyte arum colonization paris colonization pteris vittata glomus intraradices pteridophyta-gametofito esporofito colonización tipo arum colonización tipo paris |
| topic |
teris vittata glomus intraradices pteridophyta-gametophyte-sporophyte arum colonization paris colonization pteris vittata glomus intraradices pteridophyta-gametofito esporofito colonización tipo arum colonización tipo paris |
| description |
Ferns, which are usually colonizing different environments and their roots frequently present mycorrhization, have two adult stages in their life cycle, the sporophytic and the gametophytic phase. This paper describes the experimental mycorrhizal association between Pteris vittata leptosporangiate fern and a strain of Glomus intraradices during the life cycle of the fern, from spore germination to the development of a mature sporophyte. The aim of this study was to compare the colonization pattern of in vitro cultures of G. intraradices along the fern life cycle with those found in nature. For this, mature spores were obtained from fertile P. vittata fronds growing in walls of Buenos Aires city, Argentina. Roots were stained and observed under the light microscope for arbuscular mycorrhizal colonization. Approximately, 75 fern spores were cultured in each pot filled with a sterile substrate and G. intraradices (BAFC N° 51.331) as inoculum on the surface. After germination took place, samples were taken every 15 days until the fern cycle was completed. In order to determine colonization dynamics each sample was observed under optical and confocal microscope after staining. Gametophyte was classified as Adiantum type. Male and female gametangia were limited to the lower face, mycorrhizal colonization started when they were differentiated and took place through the rhizoids. Spores and vesicles were not found in this cycle stage. Paris-type mycorrhizal colonization was established in the midrib and in the embrionary foot. It was colonized by external mycelium. When the first root was developed soil inoculum colonized de novo this structure and Arum-type colonization was observed. This study proves that the type of colonization is determined by the structure of the host, not by the fungus. Both the gametophyte and embryo foot have determined growth and Paris-type colonization, while, sporophyte roots have undetermined growth and Arum-type colonization. The structures found in vitro cultures were highly similar to those found under natural conditions. |
| publishDate |
2012 |
| dc.date.none.fl_str_mv |
2012-06-01 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion artículo original http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/4019 10.15517/rbt.v60i2.4019 |
| url |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/4019 |
| identifier_str_mv |
10.15517/rbt.v60i2.4019 |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/4019/3881 |
| dc.rights.none.fl_str_mv |
Derechos de autor 2012 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 acceso abierto http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Derechos de autor 2012 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 acceso abierto http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Universidad de Costa Rica |
| publisher.none.fl_str_mv |
Universidad de Costa Rica |
| dc.source.none.fl_str_mv |
Revista de Biología Tropical; Vol. 60 No. 2 (2012): Volume 60 – Regular number 2 – June 2012; 857–865 Revista de Biología Tropical; Vol. 60 Núm. 2 (2012): Volumen 60 – Número regular 2 – Junio 2012; 857–865 Revista Biología Tropical; Vol. 60 N.º 2 (2012): Volumen 60 – Número regular 2 – Junio 2012; 857–865 2215-2075 0034-7744 10.15517/rbt.v60i2 reponame:Portal de Revistas UCR instname:Universidad de Costa Rica instacron:UCR |
| instname_str |
Universidad de Costa Rica |
| instacron_str |
UCR |
| institution |
UCR |
| reponame_str |
Portal de Revistas UCR |
| collection |
Portal de Revistas UCR |
| repository.name.fl_str_mv |
Portal de Revistas UCR - Universidad de Costa Rica |
| repository.mail.fl_str_mv |
jorge.polanco@ucr.ac.cr |
| _version_ |
1849325352818573312 |
| spelling |
Mycorrhizal association in gametophytes and sporophytes of the fern Pteris vittata (Pteridaceae) with Glomus intraradicesMycorrhizal association in gametophytes and sporophytes of the fern Pteris vittata (Pteridaceae) with Glomus intraradicesMartinez, Alicia E.Chiocchio, VivianaEm, Lo TaiRodriguez, María A.Godeas, Alicia M.teris vittataglomus intraradicespteridophyta-gametophyte-sporophytearum colonizationparis colonizationpteris vittataglomus intraradicespteridophyta-gametofitoesporofitocolonización tipo arumcolonización tipo parisFerns, which are usually colonizing different environments and their roots frequently present mycorrhization, have two adult stages in their life cycle, the sporophytic and the gametophytic phase. This paper describes the experimental mycorrhizal association between Pteris vittata leptosporangiate fern and a strain of Glomus intraradices during the life cycle of the fern, from spore germination to the development of a mature sporophyte. The aim of this study was to compare the colonization pattern of in vitro cultures of G. intraradices along the fern life cycle with those found in nature. For this, mature spores were obtained from fertile P. vittata fronds growing in walls of Buenos Aires city, Argentina. Roots were stained and observed under the light microscope for arbuscular mycorrhizal colonization. Approximately, 75 fern spores were cultured in each pot filled with a sterile substrate and G. intraradices (BAFC N° 51.331) as inoculum on the surface. After germination took place, samples were taken every 15 days until the fern cycle was completed. In order to determine colonization dynamics each sample was observed under optical and confocal microscope after staining. Gametophyte was classified as Adiantum type. Male and female gametangia were limited to the lower face, mycorrhizal colonization started when they were differentiated and took place through the rhizoids. Spores and vesicles were not found in this cycle stage. Paris-type mycorrhizal colonization was established in the midrib and in the embrionary foot. It was colonized by external mycelium. When the first root was developed soil inoculum colonized de novo this structure and Arum-type colonization was observed. This study proves that the type of colonization is determined by the structure of the host, not by the fungus. Both the gametophyte and embryo foot have determined growth and Paris-type colonization, while, sporophyte roots have undetermined growth and Arum-type colonization. The structures found in vitro cultures were highly similar to those found under natural conditions.Los helechos presentan dos etapas en su ciclo de vida, una fase esporofítica y una gametofítica. Estos por lo general pueden colonizar diferentes ambientes y frecuentemente presentan raíces micorrizadas. Este estudio describe la asociación experimental entre Pteris vittata, un helecho leptosporangiado y una cepa de Glomus intraradices durante el ciclo de vida del helecho, desde la germinación de las esporas hasta el desarrollo del esporofito maduro. El objetivo de este estudio fue comparar los patrones de colonización de G. intraradices a lo largo de todo el ciclo de vida del helecho con los tipos encontrados en la naturaleza. Las esporas maduras fueron obtenidas de frondes fértiles de P. vittata que crecen sobre las paredes de la ciudad de Buenos Aires, Argentina. Las raíces se tiñeron y fueron observadas bajo microscopio óptico para el estudio de la colonización micorrízica. Aproximadamente 75 esporas de helecho se cultivaron en macetas con un sustrato estéril y con un inóculo de G. intraradices (N° 51.331 BAFC) en la superficie. Después de la germinación, se tomaron muestras cada 15 días hasta que se completó el ciclo de vida del helecho. Con el fin de determinar la dinámica de la colonización, cada muestra se observó con el microscopio 864 óptico y el microscopio de confocal luego de la tinción correspondiente. El gametofito fue clasificado como del tipo “Adiantum”. Los gametangios femeninos y masculinos se desarrollaron en la cara inferior del mismo. La micorrización comenzó cuando los gametangios estaban ya diferenciados y la colonización se produjo a través de los rizoides. Las esporas y las vesículas no se encontraron en esta fase del ciclo. La micorrizacion tipo Paris se observó sobre la línea de la nervadura central. El pie del esporofito fue colonizado por el micelio externo. Cuando la raíz se desarrolló, se colonizó “de novo”, y se observó una colonización de tipo Arum. Este estudio demuestra que el tipo de colonización está determinado por la estructura del helecho y no por el hongo. Tanto el gametofito como el pie del embrión tienen crecimiento definido y colonización tipo Paris, mientras que las raíces del esporofito presentan un crecimiento indeterminado y una colonización tipo Arum. Las estructuras que se encontraron bajo cultivo coinciden con las que se encontraron en condiciones naturales.Universidad de Costa Rica2012-06-01info:eu-repo/semantics/publishedVersionartículo originalhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdfhttps://revistas.ucr.ac.cr/index.php/rbt/article/view/401910.15517/rbt.v60i2.4019Revista de Biología Tropical; Vol. 60 No. 2 (2012): Volume 60 – Regular number 2 – June 2012; 857–865Revista de Biología Tropical; Vol. 60 Núm. 2 (2012): Volumen 60 – Número regular 2 – Junio 2012; 857–865Revista Biología Tropical; Vol. 60 N.º 2 (2012): Volumen 60 – Número regular 2 – Junio 2012; 857–8652215-20750034-774410.15517/rbt.v60i2reponame:Portal de Revistas UCRinstname:Universidad de Costa Ricainstacron:UCRenghttps://revistas.ucr.ac.cr/index.php/rbt/article/view/4019/3881Derechos de autor 2012 Revista de Biología Tropicalhttp://creativecommons.org/licenses/by/4.0acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccess2022-06-06T19:02:58Zoai:portal.ucr.ac.cr:article/4019Portal de revistashttps://revistas.ucr.ac.cr/Universidadhttp://www.ucr.ac.crhttps://revistas.ucr.ac.cr/index.php/index/oaijorge.polanco@ucr.ac.crCosta RicaNo aplicaNo aplicaNo aplicaopendoar:2025-08-13T10:13:48.874Portal de Revistas UCR - Universidad de Costa Ricafalse |
| score |
15,812429 |