EFFICIENCY OF TRIS EXTENDERS AND BOTU-CRIO ® ON SEMINAL PARAMETERS IN STALLION BREEDS QUARTER HORSE AND MANGALARGA MARCHADOR

The limiting factor in the cryopreservation process of equine semen is related to the species, as they present a great variability in the ejaculate’s characteristics after thawing. The aim of this study was to evaluate sperm viability after thawing, from Quarter Horse and Mangalarga Marchador stalli...

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Detalles Bibliográficos
Autores: Costa, Deyse Naira Mascarenhas, Silva, Daniel Anderson Martiliano, Boakari, Yatta Linhares, Ferreira, Siluana Benvindo, Branco, Marlon Araújo Castelo, Souza, José Adalmir Torres de
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:Brasil
Institución:Universidade Federal de Goiás (UFG)
Repositorio:Ciência animal brasileira (Online)
Idioma:portugués
OAI Identifier:oai:ojs.revistas.ufg.br:article/25327
Acceso en línea:https://revistas.ufg.br/vet/article/view/25327
Access Level:acceso abierto
Palabra clave:Animal Sanity
Equino
Sêmen
Diluente
Criopreservação
Descripción
Sumario:The limiting factor in the cryopreservation process of equine semen is related to the species, as they present a great variability in the ejaculate’s characteristics after thawing. The aim of this study was to evaluate sperm viability after thawing, from Quarter Horse and Mangalarga Marchador stallions using two extenders (Botu-crio and Tris) in cryopreservation. To this end, we analyzed the physical characteristics of fresh semen, the sperm membrane functionality by the hypoosmotic swelling test (HOST), total motility and vigor by the Thermoresistance Test (TRT), progressive motility by a computerized system CASA (Computer-Assisted Semen Anlyses) and acrosomal membrane integrity and functionality of the mitochondria by epifluorescence microscopy. After thawing, the extender Botu-crio® better preserved motility, vigor and integrity of the plasma membrane. There was no significant difference between breeds for the thermoresistance test after twaing. Quarter Horse showed higher percentage of bigger defects in the sperm pathology analysis.KEYWORDS: cryopreservation, equine, extender, semen.

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