Intranasal vaccination with extracellular serine proteases of Leishmania amazonensis confers protective immunity to BALB/c mice against infection

Salvatore Giovanni De Simone - Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento.

Detalles Bibliográficos
Autores: Guedes, Herbert Leonel de Matos, Costa, Beatriz Lilian da Silva, Chaves, Suzana Passos, Gomes, Daniel Cláudio de Oliveira, Nosanchuk, Joshua Daniel, de Simone, Salvatore Giovanni, Bergmann, Bartira Rossi
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:Brasil
Institución:Fundação Oswaldo Cruz (FIOCRUZ)
Repositorio:Repositório Institucional da FIOCRUZ (ARCA)
Idioma:inglés
OAI Identifier:oai:arca.fiocruz.br:icict/10167
Acceso en línea:https://arca.fiocruz.br/handle/icict/10167
Access Level:acceso abierto
Palabra clave:Leishmania amazonensis
Murine cutaneous leishmaniasis
Mucosal vaccine
Intranasal route
Extracellular serine proteases
Leishmania
03 Saúde e Bem-Estar
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oai_identifier_str oai:arca.fiocruz.br:icict/10167
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repository_id_str
dc.title.none.fl_str_mv Intranasal vaccination with extracellular serine proteases of Leishmania amazonensis confers protective immunity to BALB/c mice against infection
title Intranasal vaccination with extracellular serine proteases of Leishmania amazonensis confers protective immunity to BALB/c mice against infection
spellingShingle Intranasal vaccination with extracellular serine proteases of Leishmania amazonensis confers protective immunity to BALB/c mice against infection
Guedes, Herbert Leonel de Matos
Leishmania amazonensis
Murine cutaneous leishmaniasis
Mucosal vaccine
Intranasal route
Extracellular serine proteases
Leishmania
Leishmania
03 Saúde e Bem-Estar
title_short Intranasal vaccination with extracellular serine proteases of Leishmania amazonensis confers protective immunity to BALB/c mice against infection
title_full Intranasal vaccination with extracellular serine proteases of Leishmania amazonensis confers protective immunity to BALB/c mice against infection
title_fullStr Intranasal vaccination with extracellular serine proteases of Leishmania amazonensis confers protective immunity to BALB/c mice against infection
title_full_unstemmed Intranasal vaccination with extracellular serine proteases of Leishmania amazonensis confers protective immunity to BALB/c mice against infection
title_sort Intranasal vaccination with extracellular serine proteases of Leishmania amazonensis confers protective immunity to BALB/c mice against infection
dc.creator.none.fl_str_mv Guedes, Herbert Leonel de Matos
Costa, Beatriz Lilian da Silva
Chaves, Suzana Passos
Gomes, Daniel Cláudio de Oliveira
Nosanchuk, Joshua Daniel
de Simone, Salvatore Giovanni
Bergmann, Bartira Rossi
author Guedes, Herbert Leonel de Matos
author_facet Guedes, Herbert Leonel de Matos
Costa, Beatriz Lilian da Silva
Chaves, Suzana Passos
Gomes, Daniel Cláudio de Oliveira
Nosanchuk, Joshua Daniel
de Simone, Salvatore Giovanni
Bergmann, Bartira Rossi
author_role author
author2 Costa, Beatriz Lilian da Silva
Chaves, Suzana Passos
Gomes, Daniel Cláudio de Oliveira
Nosanchuk, Joshua Daniel
de Simone, Salvatore Giovanni
Bergmann, Bartira Rossi
author2_role author
author
author
author
author
author
dc.subject.en.none.fl_str_mv Leishmania amazonensis
Murine cutaneous leishmaniasis
Mucosal vaccine
Intranasal route
Extracellular serine proteases
topic Leishmania amazonensis
Murine cutaneous leishmaniasis
Mucosal vaccine
Intranasal route
Extracellular serine proteases
Leishmania
Leishmania
03 Saúde e Bem-Estar
dc.subject.es.none.fl_str_mv Leishmania
dc.subject.decs.none.fl_str_mv Leishmania
dc.subject.ods.none.fl_str_mv 03 Saúde e Bem-Estar
description Salvatore Giovanni De Simone - Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-05-04T16:34:32Z
dc.date.available.fl_str_mv 2015-05-04T16:34:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GUEDES, Herbert Leonel de Matos et al. Intranasal vaccination with extracellular serine proteases of Leishmania amazonensis confers protective immunity to BALB/c mice against infection. Parasites and Vectors, v. 7, n. 448, p. 1-7, 2014.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/10167
dc.identifier.issn.none.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/1756-3305-7-448
identifier_str_mv GUEDES, Herbert Leonel de Matos et al. Intranasal vaccination with extracellular serine proteases of Leishmania amazonensis confers protective immunity to BALB/c mice against infection. Parasites and Vectors, v. 7, n. 448, p. 1-7, 2014.
1756-3305
10.1186/1756-3305-7-448
url https://arca.fiocruz.br/handle/icict/10167
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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spelling 2015-05-04T16:34:32Z2015-05-04T16:34:32Z2014GUEDES, Herbert Leonel de Matos et al. Intranasal vaccination with extracellular serine proteases of Leishmania amazonensis confers protective immunity to BALB/c mice against infection. Parasites and Vectors, v. 7, n. 448, p. 1-7, 2014.1756-3305https://arca.fiocruz.br/handle/icict/1016710.1186/1756-3305-7-448Salvatore Giovanni De Simone - Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Núcleo Multidisciplinar de Pesquisa UFRJ – Xerém em Biologia (NUMPEX-BIO). Pólo Avançado de Xerém. Duque de Caxias, RJ. Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Laboratório de Imunologia Celular e Molecular. Vitória, ES, Brasil.Yeshiva University. Albert Einstein College of Medicine. Departments of Medicine and Microbiology and Immunology. Bronx, NY, USA.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças Negligenciadas. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Background: Previously, we demonstrated that unlike subcutaneous or intramuscular vaccination, intranasal vaccination of BALB/c mice with whole Leishmania amazonensis antigens leads to protection against cutaneous leishmaniasis. Here, the role of parasite serine proteases in the protective immunity was investigated. Findings: Serine Proteases were partially purified from both soluble (LaSP-Sol) and extracellular (LaSP-Ex) Leishmania amazonensis promastigote extracts by aprotinin-agarose chromatography. BALB/c mice were intranasally immunized with LaSP-Sol and LaSP-Ex prior to infection with L. amazonensis. LaSP-Ex but not LaSP-Sol vaccination led to significantly smaller lesions and parasite burdens as compared with non-vaccinated controls. Protection was accompanied by systemic Th1 polarization with increased IFN-γ and decreased IL-4 and IL-10 splenic production. Likewise, increased production of IFN-γ, IL-12 and IL-4 concomitant with decreased TGF-β and TNF-α was locally observed in the infected footpad. Conclusion: This study indicates that extracellular serine proteases of L. amazonensis are strong candidates for a more defined intranasal vaccine against cutaneous leishmaniasis.engBioMed CentralIntranasal vaccination with extracellular serine proteases of Leishmania amazonensis confers protective immunity to BALB/c mice against infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLeishmania amazonensisMurine cutaneous leishmaniasisMucosal vaccineIntranasal routeExtracellular serine proteasesLeishmaniaLeishmania03 Saúde e Bem-Estarinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZGuedes, Herbert Leonel de MatosCosta, Beatriz Lilian da SilvaChaves, Suzana PassosGomes, Daniel Cláudio de OliveiraNosanchuk, Joshua Danielde Simone, Salvatore GiovanniBergmann, Bartira RossiLICENSElicense.txttext/plain1914https://arca.fiocruz.br/bitstreams/655b3ca5-d9eb-46dc-ab0d-d439a50e33b9/download7d48279ffeed55da8dfe2f8e81f3b81fMD51falseAnonymousREADORIGINALsalvatore_simoneetal_IOC_2014.pdfapplication/pdf520084https://arca.fiocruz.br/bitstreams/8597728c-1aae-4154-ab16-2d0955ca7d43/download36bd4ea56567dc67549f13350cfbd037MD52trueAnonymousREADTEXTsalvatore_simoneetal_IOC_2014.pdf.txtsalvatore_simoneetal_IOC_2014.pdf.txtExtracted texttext/plain66816https://arca.fiocruz.br/bitstreams/2be89f8b-4b14-49bf-967f-acb8380934a6/download94faa28fb0e7b76d098ce55312a74422MD59falseAnonymousREADTHUMBNAILsalvatore_simoneetal_IOC_2014.pdf.jpgsalvatore_simoneetal_IOC_2014.pdf.jpgGenerated Thumbnailimage/jpeg41764https://arca.fiocruz.br/bitstreams/d4daec40-736f-4763-9333-16ad2aa4ad24/downloadd8f5d7b09588b20de7d9e19d5d112d21MD510falseAnonymousREADicict/101672025-12-09 16:04:37.488open.accessoai:arca.fiocruz.br:icict/10167https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-12-09T19:04:37Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)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