ÓXIDO DE GRAFENO E ÓXIDO DE GRAFENO REDUZIDO: DA PREPARAÇÃO À VERIFICAÇÃO DA SEGURANÇA E EFICÁCIA TERAPÊUTICA IN SILICO E IN VITRO
Chronic wounds constitute a serious public health problem, as they are the main causes of limb amputation. In this way, aiming at improving the quality of life of the population, there is a need for the development of new materials, which are biologically safe, antimicrobial, low cost and effective...
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | Brasil |
| Institución: | Universidade Franciscana (UFN) |
| Repositorio: | Biblioteca Digital de Teses e Dissertações da Universidade Franciscana (UFN) |
| Idioma: | portugués |
| OAI Identifier: | oai:tede.universidadefranciscana.edu.br:UFN-BDTD/939 |
| Acceso en línea: | http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/939 |
| Access Level: | acceso embargado |
| Palabra clave: | Nanopartícula; Cicatrização; Anti-inflamatório; Antimicrobiano. Nanoparticles; Cicatrization; Anti-inflammatory; Antimicrobial. Interdisciplinar |
| Sumario: | Chronic wounds constitute a serious public health problem, as they are the main causes of limb amputation. In this way, aiming at improving the quality of life of the population, there is a need for the development of new materials, which are biologically safe, antimicrobial, low cost and effective to accelerate the healing process of chronic wounds. To present a possible new alternative for wound treatment, this work evaluated the biological safety and therapeutic efficacy of graphene oxide (GO) and reduced graphene oxide (GOr) nanoparticles (NPs). First, the nanostructures were studied in silico and showed to be able to inhibit the production of some pro-inflammatory cytokines and stimulate the production of the anti-inflammatory cytokine IL-10, especially GOr. The results of the morphological and structural characterization of GO NPs synthesized from the Hummers method and reduced by ascorbic acid, were consistent with the literature, confirming their successful achievement. In the broth microdilution assay, GO and GOr showed antimicrobial activity against the clinical isolate of Streptococcus agalactiae (S. agalactiae) at a minimum inhibitory concentration (MIC) of 625 μg/mL for GO and 312.5 μg/mL for GOr. In addition, the nanostructure of GOr was able to inhibit, in subinhibitory concentration, the formation of S. agalactiae biofilm by up to 77% when compared to the positive control. Both NPs, in all tested concentrations, did not cause hemolysis, alterations in coagulation, and DNA damage in vitro assays. However, in the safety tests, it was evidenced that only the MIC of 312, μg/mL for GOr was biologically safe and presented anti-inflammatory and healing behavior in vitro. In general, the present work confirmed GOr's potential in the treatment of chronic wounds, since in silico showed anti-inflammatory behavior and in vitro showed therapeutic efficacy at low concentrations, prevented biofilm formation, and showed no significant toxic effects. |
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