Characterization of Leishmania (L.) infantum chagasi in visceral leishmaniasis associated with hiv co-infection in Northeastern Brazil

Visceral leishmaniasis, associated with HIV/AIDS coinfection, is becoming a more aggressive disease, complicating an accurate prognosis. A 21-year-old HIV-positive female presenting with clinical features of visceral leishmaniasis was enrolled in this study. Bone marrow cytology, Novy-MacNeal-Nicoll...

Descripción completa

Detalles Bibliográficos
Autores: Lima, Elaine Cristina Bomfim de, Barbosa Júnior, Walter Lins, Brito, Maria Edileuza Felinto de, Melo, Fábio Lopes de, Brandão Filho, Sinval Pinto, Medeiros, Zulma Maria de
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Brasil
Institución:Instituto de Medicina Tropical (IMT)
Repositorio:Revista do Instituto de Medicina Tropical de São Paulo
Idioma:inglés
OAI Identifier:oai:revistas.usp.br:article/140697
Acceso en línea:https://www.revistas.usp.br/rimtsp/article/view/140697
Access Level:acceso abierto
Palabra clave:Characterization
Leishmania (L.) infantum chagasi
Visceral leishmaniasis
HIV/AIDS
Coinfection
Descripción
Sumario:Visceral leishmaniasis, associated with HIV/AIDS coinfection, is becoming a more aggressive disease, complicating an accurate prognosis. A 21-year-old HIV-positive female presenting with clinical features of visceral leishmaniasis was enrolled in this study. Bone marrow cytology, Novy-MacNeal-Nicolle culture and kDNA PCR of peripheral blood were all positive. Typing methods, multilocus enzyme electrophoresis and ITS1-RFLP PCR of peripheral blood confirmed infection by Leishmania (L.) infantum chagasi . PCR has proved to be safer and more affordable than other characterization methods; ITS1-RFLP PCR can diagnose and type Leishmania spp. in both endemic and non-endemic areas, favoring the prognosis and allowing the appropriate treatment of patients.