Combination of the essential oil constituents citral, eugenol and thymol enhance their inhibitory effect on Crithidia fasciculata and Trypanosoma cruzi growth

This work has been supported by CNPq and Fiocruz.

Detalles Bibliográficos
Autores: Azeredo, Camila Maria Oliveira de, Soares, Maurilio José
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Brasil
Institución:Fundação Oswaldo Cruz (FIOCRUZ)
Repositorio:Repositório Institucional da FIOCRUZ (ARCA)
Idioma:portugués
OAI Identifier:oai:arca.fiocruz.br:icict/15601
Acceso en línea:https://arca.fiocruz.br/handle/icict/15601
Access Level:acceso abierto
Palabra clave:Citral
Crithidia fasciculata
Eugenol
Thymol
Trypanosoma cruzi
Trypanosomatid
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dc.title.none.fl_str_mv Combination of the essential oil constituents citral, eugenol and thymol enhance their inhibitory effect on Crithidia fasciculata and Trypanosoma cruzi growth
title Combination of the essential oil constituents citral, eugenol and thymol enhance their inhibitory effect on Crithidia fasciculata and Trypanosoma cruzi growth
spellingShingle Combination of the essential oil constituents citral, eugenol and thymol enhance their inhibitory effect on Crithidia fasciculata and Trypanosoma cruzi growth
Azeredo, Camila Maria Oliveira de
Citral
Crithidia fasciculata
Eugenol
Thymol
Trypanosoma cruzi
Trypanosomatid
title_short Combination of the essential oil constituents citral, eugenol and thymol enhance their inhibitory effect on Crithidia fasciculata and Trypanosoma cruzi growth
title_full Combination of the essential oil constituents citral, eugenol and thymol enhance their inhibitory effect on Crithidia fasciculata and Trypanosoma cruzi growth
title_fullStr Combination of the essential oil constituents citral, eugenol and thymol enhance their inhibitory effect on Crithidia fasciculata and Trypanosoma cruzi growth
title_full_unstemmed Combination of the essential oil constituents citral, eugenol and thymol enhance their inhibitory effect on Crithidia fasciculata and Trypanosoma cruzi growth
title_sort Combination of the essential oil constituents citral, eugenol and thymol enhance their inhibitory effect on Crithidia fasciculata and Trypanosoma cruzi growth
dc.creator.none.fl_str_mv Azeredo, Camila Maria Oliveira de
Soares, Maurilio José
author Azeredo, Camila Maria Oliveira de
author_facet Azeredo, Camila Maria Oliveira de
Soares, Maurilio José
author_role author
author2 Soares, Maurilio José
author2_role author
dc.subject.en.none.fl_str_mv Citral
Crithidia fasciculata
Eugenol
Thymol
Trypanosoma cruzi
Trypanosomatid
topic Citral
Crithidia fasciculata
Eugenol
Thymol
Trypanosoma cruzi
Trypanosomatid
description This work has been supported by CNPq and Fiocruz.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2016-09-02T18:10:57Z
dc.date.available.fl_str_mv 2016-09-02T18:10:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv AZEREDO, Camila M. O.; SOARES, Maurilio J. Combination of the essential oil constituents citral, eugenol and thymol enhance their inhibitory effect on Crithidia fasciculata and Trypanosoma cruzi growth. Revista Brasileira de Farmacognosia, Curitiba, v. 23, n. 5, p. 762-768, Oct. 2013.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/15601
dc.identifier.issn.none.fl_str_mv 0102-695X
dc.identifier.doi.none.fl_str_mv 10.1590/S0102-695X2013000500007
identifier_str_mv AZEREDO, Camila M. O.; SOARES, Maurilio J. Combination of the essential oil constituents citral, eugenol and thymol enhance their inhibitory effect on Crithidia fasciculata and Trypanosoma cruzi growth. Revista Brasileira de Farmacognosia, Curitiba, v. 23, n. 5, p. 762-768, Oct. 2013.
0102-695X
10.1590/S0102-695X2013000500007
url https://arca.fiocruz.br/handle/icict/15601
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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spelling 2016-09-02T18:10:57Z2016-09-02T18:10:57Z2013AZEREDO, Camila M. O.; SOARES, Maurilio J. Combination of the essential oil constituents citral, eugenol and thymol enhance their inhibitory effect on Crithidia fasciculata and Trypanosoma cruzi growth. Revista Brasileira de Farmacognosia, Curitiba, v. 23, n. 5, p. 762-768, Oct. 2013.0102-695Xhttps://arca.fiocruz.br/handle/icict/1560110.1590/S0102-695X2013000500007This work has been supported by CNPq and Fiocruz.Oswaldo Cruz Fundation. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brazil.Oswaldo Cruz Fundation. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brazil.We analyzed the effect of the combination of citral, eugenol and thymol, respectively the main constituents of essential oils of Cympobogon citratus (DC) Stapf, Poaceae (lemon grass), Syzygium aromaticum (L.) Merr. & L.M. Perry, Myrtaceae (clove) and Thymus vulgaris L., Lamiaceae (thyme), on the proliferation of the trypanosomatids Crithidia fasciculata and Trypanosoma cruzi. The constituents were initially added individually at different concentrations to C. fasciculata cultures to estimate the IC50/24h. Concentrations in a triple combination were about 2 times and 16.5 times lower against C. fasciculata and T. cruzi, respectively, as compared to isolated compounds. Incubation of C. fasciculata with the trypanocydal agent benznidazole did not affect parasite growth at concentrations up to 500 µg/ml, but the IC50 of this drug against T. cruziwas 15.8 µg/ml, a value about 2-5 times higher than that of constituents in the triple combination. Analysis of treated C. fasciculata by scanning electron microscopy showed rounding of the cell body. Our data show that combination of essential oil constituents resulted in increased inhibitory activity on growth of both non-pathogenic and pathogenic trypanosomatid species and indicate that the non-patogenic C. fasciculata may represent a resistant model for drug screening in trypanosomatids.porCombination of the essential oil constituents citral, eugenol and thymol enhance their inhibitory effect on Crithidia fasciculata and Trypanosoma cruzi growthinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCitralCrithidia fasciculataEugenolThymolTrypanosoma cruziTrypanosomatidinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZAzeredo, Camila Maria Oliveira deSoares, Maurilio JoséLICENSElicense.txtlicense.txttext/plain; charset=utf-83074https://arca.fiocruz.br/bitstreams/99d8b12e-645f-45da-a919-610ddcee4201/download0271fb68963c017aa933c7aff60379f4MD51falseAnonymousREADORIGINALCombination of the essential oil constituents citral, eugenol and thymol.pdfCombination of the essential oil constituents citral, eugenol and thymol.pdfapplication/pdf895900https://arca.fiocruz.br/bitstreams/b2bcbd26-2f48-42a8-898b-1d349dd151a1/download32525205d511b6991bbd8e277807f893MD52trueAnonymousREADTEXTCombination of the essential oil constituents citral, eugenol and thymol.pdf.txtCombination of the essential oil constituents citral, eugenol and thymol.pdf.txtExtracted texttext/plain34519https://arca.fiocruz.br/bitstreams/28ddcc25-49ca-473d-9c82-249f901aff2a/downloade1e53c6c639191af865cd647e8f0c91fMD57falseAnonymousREADTHUMBNAILCombination of the essential oil constituents citral, eugenol and thymol.pdf.jpgCombination of the essential oil constituents citral, eugenol and thymol.pdf.jpgGenerated Thumbnailimage/jpeg40114https://arca.fiocruz.br/bitstreams/a3c55f6f-9e18-499e-a98b-4f6dd92f8e05/download3b558197fe1532274893d425b1b79d0eMD58falseAnonymousREADicict/156012025-12-10 13:48:06.771open.accessoai:arca.fiocruz.br:icict/15601https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-12-10T16:48:06Repositório Institucional da FIOCRUZ (ARCA) - 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