Calcium and caffeine interaction in increased calcium balance in ovariectomized rats

ObjectiveThis study investigated the effects of caffeine intake associated with inadequate or adequate calcium intake in laparotomized or ovariectomized rats by means of the calcium balance. Forty adults Wistar rats were ovariectomized or laparotomized. MethodsThe animals (n=40) were randomly placed...

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Detalles Bibliográficos
Autores: Tavares da SILVA, Sandra, Brunoro COSTA, Neuza Maria, Souza Lima Caldoncelli FRANCO, Frederico, NATALI, Antônio José
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:Brasil
Institución:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)
Repositorio:Revista de Nutrição
Idioma:inglés
OAI Identifier:oai:ojs.periodicos.puc-campinas.edu.br:article/8428
Acceso en línea:https://periodicos.puc-campinas.edu.br/nutricao/article/view/8428
Access Level:acceso abierto
Palabra clave:Caffeine
Calcium bioavailability
Estrogen
Cafeína
Biodisponibilidade do cálcio
Estrogênio
Descripción
Sumario:ObjectiveThis study investigated the effects of caffeine intake associated with inadequate or adequate calcium intake in laparotomized or ovariectomized rats by means of the calcium balance. Forty adults Wistar rats were ovariectomized or laparotomized. MethodsThe animals (n=40) were randomly placed in eight groups receiving the AIN-93 diet with 100% or 50% of the recommended calcium intake with or without added caffeine (6mg/kg/day). The animals were kept in individuals metabolic cages at a temperature of 24°±2o C, light/dark cycles of 12/12 hours, and deionized water available ad libitum. On the 8th week of the experiment, food consumption was measured and 24-hour urine and 4-day feces were collected to determine calcium balance [Balance=Ca intake-(Urinary Ca+Fecal Ca)]. ResultsAnimals with adequate calcium intake presented higher balances and rates of calcium absorption and retention (p<0.05) than those with inadequate calcium intake, regardless of caffeine intake (p<0.05). Caffeine intake did not affect urinary calcium excretion but increased balance (p<0.05) in the groups with adequate calcium intake. ConclusionAdequate calcium intake attenuated the negative effects of estrogen deficiency and improved calcium balance even in the presence of caffeine.