Histological effect caused by contrast solution injection for post mortem computed tomography angiography in dogs
The post mortem computed tomography angiography (PMCTA) is a technique that uses a contrast solution to evaluate the vascular structures in cadavers. This study aims to verify if there is any tissue impairment in parenchymatous organs after PMCTA in dogs. PMCTA was performed in 9 cadavers, divided i...
| Autores: | , , , , , , |
|---|---|
| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | Brasil |
| Recursos: | Universidade Estadual Paulista (UNESP) |
| Repositorio: | Repositório Institucional da UNESP |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unesp.br:11449/228901 |
| Acesso em linha: | http://dx.doi.org/10.1016/j.fri.2021.200435 http://hdl.handle.net/11449/228901 |
| Access Level: | acceso abierto |
| Palavra-chave: | Canine Post mortem angiotomography Post mortem biopsy Post mortem diagnostic imaging Post mortem tissue sampling Virtual necropsy |
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Histological effect caused by contrast solution injection for post mortem computed tomography angiography in dogsCaninePost mortem angiotomographyPost mortem biopsyPost mortem diagnostic imagingPost mortem tissue samplingVirtual necropsyThe post mortem computed tomography angiography (PMCTA) is a technique that uses a contrast solution to evaluate the vascular structures in cadavers. This study aims to verify if there is any tissue impairment in parenchymatous organs after PMCTA in dogs. PMCTA was performed in 9 cadavers, divided into 3 groups. There is 23% volemia of contrast solution in groups 1 and 2, and 46% in group 3. Iodine contrast was diluted in polyethylene glycol in groups 1 and 3, and in soybean oil in group 2. Tissue samples were collected from lungs, liver and kidney before and after the PMCTA. The contrast perfusion of each target-organ was measured through CT images by the increased attenuation of Hounsfield units. Each histological sample was evaluated according to its correspondence with the sampling organ and its quality. The presence of artifacts was also assessed and the histological findings were described. The sample quality was attributed a score between 0 and 2. The histological findings were compared for samples collected before and after the PMCTA procedure, and the similarity degree for each pair was calculated. The means of attenuation increase, quality score, and similarity degree were 53, 31 and 157 HU; 1.6, 1.9 and 1.5; and 67%, 62% and 56% for groups 1,2 and 3 respectively. No histological artifact or impairment was noticed in the histological evaluation of samples collected after PMCTA. Therefore, it can be concluded that histological evaluation can be performed after PMCTA to support the post mortem diagnosis.School of Veterinary Medicine and Animal Sciences of University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade UniversitáriaSchool of Veterinary Medicine and Animal Sciences of Federal University of BahiaDepartment of Veterinary Clinical Sciences School of Veterinary Medicine and Animal Science São Paulo State University –Department of Veterinary Clinical Sciences School of Veterinary Medicine and Animal Science São Paulo State University –Universidade de São Paulo (USP)Universidade Federal da Bahia (UFBA)Universidade Estadual Paulista (UNESP)2022-04-29T08:29:21Z2022-04-29T08:29:21Z2021-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.fri.2021.200435Forensic Imaging, v. 24.2666-22562666-2264http://hdl.handle.net/11449/22890110.1016/j.fri.2021.2004352-s2.0-85100629552Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengForensic Imaginginfo:eu-repo/semantics/openAccessFerrante, B.Baroni, C. O.Muramoto, C.Ribas, L. M.M. [UNESP]Torres, L.Catão-Dias, J. L.Pinto, A. C.B.C.F.2025-10-23T05:03:38Zoai:repositorio.unesp.br:11449/228901Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-10-23T05:03:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
Histological effect caused by contrast solution injection for post mortem computed tomography angiography in dogs |
| title |
Histological effect caused by contrast solution injection for post mortem computed tomography angiography in dogs |
| spellingShingle |
Histological effect caused by contrast solution injection for post mortem computed tomography angiography in dogs Ferrante, B. Canine Post mortem angiotomography Post mortem biopsy Post mortem diagnostic imaging Post mortem tissue sampling Virtual necropsy |
| title_short |
Histological effect caused by contrast solution injection for post mortem computed tomography angiography in dogs |
| title_full |
Histological effect caused by contrast solution injection for post mortem computed tomography angiography in dogs |
| title_fullStr |
Histological effect caused by contrast solution injection for post mortem computed tomography angiography in dogs |
| title_full_unstemmed |
Histological effect caused by contrast solution injection for post mortem computed tomography angiography in dogs |
| title_sort |
Histological effect caused by contrast solution injection for post mortem computed tomography angiography in dogs |
| dc.creator.none.fl_str_mv |
Ferrante, B. Baroni, C. O. Muramoto, C. Ribas, L. M.M. [UNESP] Torres, L. Catão-Dias, J. L. Pinto, A. C.B.C.F. |
| author |
Ferrante, B. |
| author_facet |
Ferrante, B. Baroni, C. O. Muramoto, C. Ribas, L. M.M. [UNESP] Torres, L. Catão-Dias, J. L. Pinto, A. C.B.C.F. |
| author_role |
author |
| author2 |
Baroni, C. O. Muramoto, C. Ribas, L. M.M. [UNESP] Torres, L. Catão-Dias, J. L. Pinto, A. C.B.C.F. |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Federal da Bahia (UFBA) Universidade Estadual Paulista (UNESP) |
| dc.subject.por.fl_str_mv |
Canine Post mortem angiotomography Post mortem biopsy Post mortem diagnostic imaging Post mortem tissue sampling Virtual necropsy |
| topic |
Canine Post mortem angiotomography Post mortem biopsy Post mortem diagnostic imaging Post mortem tissue sampling Virtual necropsy |
| description |
The post mortem computed tomography angiography (PMCTA) is a technique that uses a contrast solution to evaluate the vascular structures in cadavers. This study aims to verify if there is any tissue impairment in parenchymatous organs after PMCTA in dogs. PMCTA was performed in 9 cadavers, divided into 3 groups. There is 23% volemia of contrast solution in groups 1 and 2, and 46% in group 3. Iodine contrast was diluted in polyethylene glycol in groups 1 and 3, and in soybean oil in group 2. Tissue samples were collected from lungs, liver and kidney before and after the PMCTA. The contrast perfusion of each target-organ was measured through CT images by the increased attenuation of Hounsfield units. Each histological sample was evaluated according to its correspondence with the sampling organ and its quality. The presence of artifacts was also assessed and the histological findings were described. The sample quality was attributed a score between 0 and 2. The histological findings were compared for samples collected before and after the PMCTA procedure, and the similarity degree for each pair was calculated. The means of attenuation increase, quality score, and similarity degree were 53, 31 and 157 HU; 1.6, 1.9 and 1.5; and 67%, 62% and 56% for groups 1,2 and 3 respectively. No histological artifact or impairment was noticed in the histological evaluation of samples collected after PMCTA. Therefore, it can be concluded that histological evaluation can be performed after PMCTA to support the post mortem diagnosis. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-03-01 2022-04-29T08:29:21Z 2022-04-29T08:29:21Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.fri.2021.200435 Forensic Imaging, v. 24. 2666-2256 2666-2264 http://hdl.handle.net/11449/228901 10.1016/j.fri.2021.200435 2-s2.0-85100629552 |
| url |
http://dx.doi.org/10.1016/j.fri.2021.200435 http://hdl.handle.net/11449/228901 |
| identifier_str_mv |
Forensic Imaging, v. 24. 2666-2256 2666-2264 10.1016/j.fri.2021.200435 2-s2.0-85100629552 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Forensic Imaging |
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info:eu-repo/semantics/openAccess |
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openAccess |
| dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Universidade Estadual Paulista (UNESP) |
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UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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repositoriounesp@unesp.br |
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