Could the saponin hemolysate be used for the hemoglobin A2 dosage ?
It was made the evalution of the hemoglobin A2(Hb A2) determination using four hemolysate types. In the 1st. stage of the evalution thirty eterminations were proceeded for fraction of Hb A2 starting from hemolysate with saponine and starting from carbon tetrachloride, in the 2nd stage more 40 deter...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2003 |
| País: | Brasil |
| Institución: | Instituto Adolfo Lutz |
| Repositorio: | Revista do Instituto Adolfo Lutz (Online) |
| Idioma: | portugués |
| OAI Identifier: | oai:ojs.periodicos.saude.sp.gov.br:article/34944 |
| Acceso en línea: | https://periodicos.saude.sp.gov.br/RIAL/article/view/34944 |
| Access Level: | acceso abierto |
| Palabra clave: | Hemoglobin A2 hemolysate hemoglobinophaties electrophoresis Hemoglobina A hemolisado eletroforese hemoglobinopatias |
| Sumario: | It was made the evalution of the hemoglobin A2(Hb A2) determination using four hemolysate types. In the 1st. stage of the evalution thirty eterminations were proceeded for fraction of Hb A2 starting from hemolysate with saponine and starting from carbon tetrachloride, in the 2nd stage more 40 determinations were analyzed using hemolysate obtained through saponine and with cloroform, however added with potassium cyanide (KCN). The statistical analysis done through test t in the 1st , it revealed atendency of the results obtained starting from saponine are larger about 0,42% that those obtained starting from carbon tetrachloride (p<0,001). However, when KCN is added in the hemolysate there is no significant difference among the classic hemolisado and with the saponine. Like this, the use of the classic hemolysateor of the saponine with KCN are indicative for Hb A2 quantitative determination for the method of elution. |
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