Differential expression of VuUCP1a and VuUCP1b in caupi under salt stress

Salt stress affects growth and development of plants, inducing a variety of physiological and biochemical responses as an adaptation mechanism for survival. The plant uncoupling mitochondrial proteins (pUCPs) are able to dissipate the proton electrochemical gradient as heat and are encoded by a mult...

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Authors: Garantizado, Francisco, Costa, José, Maia, Ivan, Melo, Dirce
Format: article
Status:Published version
Publication Date:2011
Country:Brasil
Institution:Universidade Federal do Ceará (UFC)
Repository:Revista ciência agronômica (Online)
Language:English
OAI Identifier:oai:periodicos.ufc:article/84127
Online Access:http://periodicos.ufc.br/revistacienciaagronomica/article/view/84127
Access Level:Open access
Keyword:Salt stress
pUCP
Vigna unguiculata
Estresse salino
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spelling Differential expression of VuUCP1a and VuUCP1b in caupi under salt stressExpressão diferencial dos genes VuUCP1a e VuUCP1b em caupi sob estresse salinoSalt stress pUCP Vigna unguiculataEstresse salino pUCP Vigna unguiculataSalt stress affects growth and development of plants, inducing a variety of physiological and biochemical responses as an adaptation mechanism for survival. The plant uncoupling mitochondrial proteins (pUCPs) are able to dissipate the proton electrochemical gradient as heat and are encoded by a multigene family. They works as defence systems avoiding the formation of reactive oxygen species promoted by environmental stress. The aim of this work was to study gene expression of pUCPs (VuUCP1a and VuUCP1b) in roots and leaves from Vigna unguiculata seedlings under salt stress (100 mM NaCl). Seeds were germinated in the dark and after 3 days, the seedlings were transferred to Hoagland’s medium and grown for 3 additional days before being submitted to the stress condition. Roots and leaves were harvested at 0; 6; 12 and 24 hours after addition of NaCl for total RNA isolation and RT-PCR assays. Expression analysis by RT-PCR showed that VuUCP1a is constitutive in leaves and roots while VuUCP1b is expressed as tissue-dependent presenting a constitutive profile in leaves and a differential one in roots from seedlings under salt stress. The uniqueness of pUCP1 gene duplication in cowpea with differential expression suggest a role of this enzyme in the adjustment of salt stress as well as promotes this species as an attractive model to understand the role of pUCP gene members in plants.O estresse salino afeta o crescimento e o desenvolvimento das plantas, induzindo respostas bioquímicas e fisiológicas como mecanismo de adaptação para sua sobrevivência. As proteínas desacopladoras de planta (pUCPs) dissipam o gradiente eletroquímico mitocondrial de prótons como calor e são codificadas por famílias multigênicas. Elas atuam como sistema de defesa evitando a formação de espécies reativas de oxigênio geradas em resposta a estresses ambientais. O objetivo do trabalho foi estudar a expressão de genes das pUCPs (VuUCP1a e VuUCP1b ) em raízes e folhas de plântulas de Vigna unguiculata submetidas ao estresse salino (NaCl 100 mM). Sementes foram germinadas no escuro e após 3 dias, as plântulas foram transferidas para solução de Hoagland, permanecendo 3 dias antes da aplicação do estresse. As raízes e folhas foram coletadas com 0; 6; 12 e 24 horas, após adição de NaCl, para avaliar o perfil de expressão dos genes da pUCP por RT- PCR. A análise de transcritos mostrou que VuUCP1a é expresso em raízes e folhas revelando um perfil constitutivo enquanto que VuUCP1b é dependente do tecido, isto é, expresso nas folhas, sem alteração em resposta ao estresse e induzido pelo estresse salino nas raízes. A peculiaridade da duplicação gênica de pUCP1 em caupi com perfil diferencial de expressão, sugere tanto um papel dessa enzima nos mecanismos de ajustamento ao estresse salino quanto promove essa espécie como um modelo atrativo para compreensão do papel da família multigênica da pUCP em plantas.Revista Ciência Agronômica2011-04-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://periodicos.ufc.br/revistacienciaagronomica/article/view/84127Revista Ciência Agronômica; v. 42 n. 2 (2011)1806-66900045-6888reponame:Revista ciência agronômica (Online)instname:Universidade Federal do Ceará (UFC)instacron:UFCenghttp://periodicos.ufc.br/revistacienciaagronomica/article/view/84127/228279info:eu-repo/semantics/openAccessGarantizado, FranciscoCosta, JoséMaia, IvanMelo, Dirce2023-02-28T23:57:30Zoai:periodicos.ufc:article/84127Revistahttps://periodicos.ufc.br/revistacienciaagronomicaPUBhttps://periodicos.ufc.br/revistacienciaagronomica/oai||alekdutra@ufc.br|| ccarev@ufc.br1806-66900045-6888opendoar:2023-02-28T23:57:30Revista ciência agronômica (Online) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Differential expression of VuUCP1a and VuUCP1b in caupi under salt stress
Expressão diferencial dos genes VuUCP1a e VuUCP1b em caupi sob estresse salino
title Differential expression of VuUCP1a and VuUCP1b in caupi under salt stress
spellingShingle Differential expression of VuUCP1a and VuUCP1b in caupi under salt stress
Garantizado, Francisco
Salt stress
pUCP
Vigna unguiculata
Estresse salino
pUCP
Vigna unguiculata
title_short Differential expression of VuUCP1a and VuUCP1b in caupi under salt stress
title_full Differential expression of VuUCP1a and VuUCP1b in caupi under salt stress
title_fullStr Differential expression of VuUCP1a and VuUCP1b in caupi under salt stress
title_full_unstemmed Differential expression of VuUCP1a and VuUCP1b in caupi under salt stress
title_sort Differential expression of VuUCP1a and VuUCP1b in caupi under salt stress
dc.creator.none.fl_str_mv Garantizado, Francisco
Costa, José
Maia, Ivan
Melo, Dirce
author Garantizado, Francisco
author_facet Garantizado, Francisco
Costa, José
Maia, Ivan
Melo, Dirce
author_role author
author2 Costa, José
Maia, Ivan
Melo, Dirce
author2_role author
author
author
dc.subject.por.fl_str_mv Salt stress
pUCP
Vigna unguiculata
Estresse salino
pUCP
Vigna unguiculata
topic Salt stress
pUCP
Vigna unguiculata
Estresse salino
pUCP
Vigna unguiculata
description Salt stress affects growth and development of plants, inducing a variety of physiological and biochemical responses as an adaptation mechanism for survival. The plant uncoupling mitochondrial proteins (pUCPs) are able to dissipate the proton electrochemical gradient as heat and are encoded by a multigene family. They works as defence systems avoiding the formation of reactive oxygen species promoted by environmental stress. The aim of this work was to study gene expression of pUCPs (VuUCP1a and VuUCP1b) in roots and leaves from Vigna unguiculata seedlings under salt stress (100 mM NaCl). Seeds were germinated in the dark and after 3 days, the seedlings were transferred to Hoagland’s medium and grown for 3 additional days before being submitted to the stress condition. Roots and leaves were harvested at 0; 6; 12 and 24 hours after addition of NaCl for total RNA isolation and RT-PCR assays. Expression analysis by RT-PCR showed that VuUCP1a is constitutive in leaves and roots while VuUCP1b is expressed as tissue-dependent presenting a constitutive profile in leaves and a differential one in roots from seedlings under salt stress. The uniqueness of pUCP1 gene duplication in cowpea with differential expression suggest a role of this enzyme in the adjustment of salt stress as well as promotes this species as an attractive model to understand the role of pUCP gene members in plants.
publishDate 2011
dc.date.none.fl_str_mv 2011-04-27
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://periodicos.ufc.br/revistacienciaagronomica/article/view/84127
url http://periodicos.ufc.br/revistacienciaagronomica/article/view/84127
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://periodicos.ufc.br/revistacienciaagronomica/article/view/84127/228279
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Revista Ciência Agronômica
publisher.none.fl_str_mv Revista Ciência Agronômica
dc.source.none.fl_str_mv Revista Ciência Agronômica; v. 42 n. 2 (2011)
1806-6690
0045-6888
reponame:Revista ciência agronômica (Online)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Revista ciência agronômica (Online)
collection Revista ciência agronômica (Online)
repository.name.fl_str_mv Revista ciência agronômica (Online) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv ||alekdutra@ufc.br|| ccarev@ufc.br
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score 15,228081

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