Cloning and characterization of a LON gene homologue from the human pathogen Paracoccidioides brasiliensis
A LON gene homologue from the human pathogen Paracoccidioides brasiliensis (PbLON) has been cloned, sequenced and characterized. It encodes a putative ATP-dependent proteinase Lon, which in Saccharomyces cerevisisae (PIM1) is a heat-inducible protein involved in the degradation of abnormal or short-...
| Autores: | , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2001 |
| País: | Brasil |
| Institución: | Universidade Federal de São Paulo (UNIFESP) |
| Repositorio: | Repositório Institucional da UNIFESP |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unifesp.br:11600/26584 |
| Acceso en línea: | http://dx.doi.org/10.1002/yea.745 http://repositorio.unifesp.br/handle/11600/26584 |
| Access Level: | acceso abierto |
| Palabra clave: | LON homologue Paracoccidiodes brasiliensis heat shock protein ATP-dependent proteinase MDJ1 homologue |
| Sumario: | A LON gene homologue from the human pathogen Paracoccidioides brasiliensis (PbLON) has been cloned, sequenced and characterized. It encodes a putative ATP-dependent proteinase Lon, which in Saccharomyces cerevisisae (PIM1) is a heat-inducible protein involved in the degradation of abnormal or short-lived proteins in the mitochondria. the PbLON ORF is within a 3369 bp fragment interrupted by two introns located in the 3 ' segment. the 5 ' and 3 ' regions flanking the ORF contain sequences which resemble known transcription elements. Several transcription binding factor motifs have also been found, including sites for heat shock/stress response and nitrogen control, the deduced protein consists of 1063 residues containing a mitochondrial import signal at the N-terminus and conserved ATP-binding (GPPGVGKT) and serine catalytic (KDGPSAG) sites. It shares high identity with Lon homologues from S. cerevisiae (73%), Homo sapiens (62%) and Escherichia coli (56%). in P. brasiliensis, an MDJ1 putative gene has also been partially sequenced adjacent to PbLON, possibly sharing divergently orientated promoter elements, This chromosomal organization is interesting, since Mdj1p is a heat shock chaperone essential for substrate degradation by PIM1 in yeast. the LON nucleotide and flanking sequence regions have been submitted to GenBank under Accession No. AF239178, Copyright (C) 2001 John Wiley & Sons, Ltd. |
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