A study of cross-reactivity in serum samples from dogs positive for Leishmania sp., Babesia canis and ehrlichia canis in enzyme-linked immunosorbent assay and indirect fluorescent antibody test

To verify the presence of cross-reaction among leishmaniosis, ehrlichiosis and babesiosis in serological diagnostics used in human visceral leishmaniasis control programs, serum samples from leishmaniasis endemic and non-endemic areas were collected and tested by Indirect Fluorescent Antibody (IFAT)...

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Detalhes bibliográficos
Autores: Oliveira, Trícia Maria F. de Sousa [UNESP], Furuta, Patrícia I. [UNESP], de Carvalho, Débora, Machado, Rosangela Z.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:Brasil
Recursos:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/231221
Acesso em linha:http://hdl.handle.net/11449/231221
Access Level:acceso abierto
Palavra-chave:Babesia canis
Ehrlichia canis
Elisa
IFAT
Leishmania sp.
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spelling A study of cross-reactivity in serum samples from dogs positive for Leishmania sp., Babesia canis and ehrlichia canis in enzyme-linked immunosorbent assay and indirect fluorescent antibody testBabesia canisEhrlichia canisElisaIFATLeishmania sp.To verify the presence of cross-reaction among leishmaniosis, ehrlichiosis and babesiosis in serological diagnostics used in human visceral leishmaniasis control programs, serum samples from leishmaniasis endemic and non-endemic areas were collected and tested by Indirect Fluorescent Antibody (IFAT) and Enzyme-linked immunosorbent assay (ELISA). All serum samples from endemic areas were positive for Leishmania sp., by ELISA and IFAT, 51% positive for Babesia canis and 43% for Ehrlichia canis by IFAT. None of the serum samples from non-endemic areas were positive for Leishmania sp., by IFAT, but 67% were positive for B. canis and 78% for E. canis using the same test. When tested by ELISA for Leishmania sp., four samples from non-endemic area were positive. These dogs were then located and no clinical signs, parasites or antibody was detected in new tests for a six month period. Only one of these 4 samples was positive for B. canis by IFAT and ELISA and three for E. canis by IFAT. The results of the work suggest a co-infection in the endemic area and no serological cross-reaction among these parasites by IFAT and ELISA.Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista, Jaboticabal, SP 14884-900Universidade Federal de São Carlos, São Carlos-SPDepartamento de Patologia Veterinária, Rodovia Paulo Donato Castellane, Km 5, Jaboticabal, SP 14884-900Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista, Jaboticabal, SP 14884-900Universidade Estadual Paulista (UNESP)Universidade Federal de São Carlos (UFSCar)2022-04-29T08:44:14Z2022-04-29T08:44:14Z2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7-11Revista Brasileira de Parasitologia Veterinaria, v. 17, n. 1, p. 7-11, 2008.0103-846Xhttp://hdl.handle.net/11449/2312212-s2.0-73149084436Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Parasitologia Veterinariainfo:eu-repo/semantics/openAccessOliveira, Trícia Maria F. de Sousa [UNESP]Furuta, Patrícia I. [UNESP]de Carvalho, DéboraMachado, Rosangela Z.2024-06-07T13:02:37Zoai:repositorio.unesp.br:11449/231221Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-06-07T13:02:37Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A study of cross-reactivity in serum samples from dogs positive for Leishmania sp., Babesia canis and ehrlichia canis in enzyme-linked immunosorbent assay and indirect fluorescent antibody test
title A study of cross-reactivity in serum samples from dogs positive for Leishmania sp., Babesia canis and ehrlichia canis in enzyme-linked immunosorbent assay and indirect fluorescent antibody test
spellingShingle A study of cross-reactivity in serum samples from dogs positive for Leishmania sp., Babesia canis and ehrlichia canis in enzyme-linked immunosorbent assay and indirect fluorescent antibody test
Oliveira, Trícia Maria F. de Sousa [UNESP]
Babesia canis
Ehrlichia canis
Elisa
IFAT
Leishmania sp.
title_short A study of cross-reactivity in serum samples from dogs positive for Leishmania sp., Babesia canis and ehrlichia canis in enzyme-linked immunosorbent assay and indirect fluorescent antibody test
title_full A study of cross-reactivity in serum samples from dogs positive for Leishmania sp., Babesia canis and ehrlichia canis in enzyme-linked immunosorbent assay and indirect fluorescent antibody test
title_fullStr A study of cross-reactivity in serum samples from dogs positive for Leishmania sp., Babesia canis and ehrlichia canis in enzyme-linked immunosorbent assay and indirect fluorescent antibody test
title_full_unstemmed A study of cross-reactivity in serum samples from dogs positive for Leishmania sp., Babesia canis and ehrlichia canis in enzyme-linked immunosorbent assay and indirect fluorescent antibody test
title_sort A study of cross-reactivity in serum samples from dogs positive for Leishmania sp., Babesia canis and ehrlichia canis in enzyme-linked immunosorbent assay and indirect fluorescent antibody test
dc.creator.none.fl_str_mv Oliveira, Trícia Maria F. de Sousa [UNESP]
Furuta, Patrícia I. [UNESP]
de Carvalho, Débora
Machado, Rosangela Z.
author Oliveira, Trícia Maria F. de Sousa [UNESP]
author_facet Oliveira, Trícia Maria F. de Sousa [UNESP]
Furuta, Patrícia I. [UNESP]
de Carvalho, Débora
Machado, Rosangela Z.
author_role author
author2 Furuta, Patrícia I. [UNESP]
de Carvalho, Débora
Machado, Rosangela Z.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Federal de São Carlos (UFSCar)
dc.subject.por.fl_str_mv Babesia canis
Ehrlichia canis
Elisa
IFAT
Leishmania sp.
topic Babesia canis
Ehrlichia canis
Elisa
IFAT
Leishmania sp.
description To verify the presence of cross-reaction among leishmaniosis, ehrlichiosis and babesiosis in serological diagnostics used in human visceral leishmaniasis control programs, serum samples from leishmaniasis endemic and non-endemic areas were collected and tested by Indirect Fluorescent Antibody (IFAT) and Enzyme-linked immunosorbent assay (ELISA). All serum samples from endemic areas were positive for Leishmania sp., by ELISA and IFAT, 51% positive for Babesia canis and 43% for Ehrlichia canis by IFAT. None of the serum samples from non-endemic areas were positive for Leishmania sp., by IFAT, but 67% were positive for B. canis and 78% for E. canis using the same test. When tested by ELISA for Leishmania sp., four samples from non-endemic area were positive. These dogs were then located and no clinical signs, parasites or antibody was detected in new tests for a six month period. Only one of these 4 samples was positive for B. canis by IFAT and ELISA and three for E. canis by IFAT. The results of the work suggest a co-infection in the endemic area and no serological cross-reaction among these parasites by IFAT and ELISA.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
2022-04-29T08:44:14Z
2022-04-29T08:44:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Revista Brasileira de Parasitologia Veterinaria, v. 17, n. 1, p. 7-11, 2008.
0103-846X
http://hdl.handle.net/11449/231221
2-s2.0-73149084436
identifier_str_mv Revista Brasileira de Parasitologia Veterinaria, v. 17, n. 1, p. 7-11, 2008.
0103-846X
2-s2.0-73149084436
url http://hdl.handle.net/11449/231221
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Brasileira de Parasitologia Veterinaria
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 7-11
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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score 15,301629

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