Determination of germ tube, phospholipase, and proteinase production by bloodstream isolates of Candida albicans

Candida albicans is a commensal and opportunistic agent that causes infection in immunocompromised individuals. Several attributes contribute to the virulence and pathogenicity of this yeast, including the production of germ tubes (GTs) and extracellular hydrolytic enzymes, particularly phospholipas...

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Autores: Mattei, Antonella Souza, Alves, Sydney Hartz, Severo, Cecília Bittencourt, Guazzelli, Luciana Silva, Oliveira, Flávio de Mattos, Severo, Luiz Carlos
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Brasil
Recursos:Universidade Federal do Rio Grande do Sul (UFRGS)
Repositorio:Repositório Institucional da UFRGS
Idioma:inglés
OAI Identifier:oai:www.lume.ufrgs.br:10183/94965
Acesso em linha:http://hdl.handle.net/10183/94965
Access Level:acceso abierto
Palavra-chave:Virulência
Fosfolipases
Peptídeo hidrolases
Candida albicans
Candidemia
Phospholipase
Proteinase
Germ tube
Virulence
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spelling 2014-05-07T02:04:32Z20130037-8682http://hdl.handle.net/10183/94965000898157Candida albicans is a commensal and opportunistic agent that causes infection in immunocompromised individuals. Several attributes contribute to the virulence and pathogenicity of this yeast, including the production of germ tubes (GTs) and extracellular hydrolytic enzymes, particularly phospholipase and proteinase. This study aimed to investigate GT production and phospholipase and proteinase activities in bloodstream isolates of C. albicans. Methods: One hundred fi fty-three C. albicans isolates were obtained from blood samples and analyzed for GT, phospholipase, and proteinase production. The assays were performed in duplicate in egg yolk medium containing bovine serum albumin and human serum. Results: Detectable amounts of proteinase were produced by 97% of the isolates, and 78% of the isolates produced phospholipase. GTs were produced by 95% of the isolates. A majority of the isolates exhibited low levels of phospholipase production and high levels of proteinase production. Conclusions: Bloodstream isolates of C. albicans produce virulence factors such as GT and hydrolytic enzymes that enable them to cause infection under favorable conditions.application/pdfengRevista da Sociedade Brasileira de Medicina Tropical. Brasilia. Vol. 46, n. 3 (maio/jun. 2013), p. 340-342.VirulênciaFosfolipasesPeptídeo hidrolasesCandida albicansCandidemiaPhospholipaseCandida albicansCandidemiaProteinaseGerm tubeVirulenceDetermination of germ tube, phospholipase, and proteinase production by bloodstream isolates of Candida albicansEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSMattei, Antonella SouzaAlves, Sydney HartzSevero, Cecília BittencourtGuazzelli, Luciana SilvaOliveira, Flávio de MattosSevero, Luiz CarlosORIGINAL000898157.pdf000898157.pdfTexto completo (inglês)application/pdf676841http://www.lume.ufrgs.br/bitstream/10183/94965/1/000898157.pdf0250315a482688b335839f1e6d476eb4MD51TEXT000898157.pdf.txt000898157.pdf.txtExtracted Texttext/plain15421http://www.lume.ufrgs.br/bitstream/10183/94965/2/000898157.pdf.txt03645ed688206693d2bed6e5ece8136aMD52THUMBNAIL000898157.pdf.jpg000898157.pdf.jpgGenerated Thumbnailimage/jpeg2332http://www.lume.ufrgs.br/bitstream/10183/94965/3/000898157.pdf.jpgf64090aecb3a47e0a6495d65d9c6e48eMD5310183/949652021-07-09 04:33:39.851239oai:www.lume.ufrgs.br:10183/94965Repositório InstitucionalPUBhttps://lume.ufrgs.br/oai/requestlume@ufrgs.bropendoar:2021-07-09T07:33:39Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Determination of germ tube, phospholipase, and proteinase production by bloodstream isolates of Candida albicans
title Determination of germ tube, phospholipase, and proteinase production by bloodstream isolates of Candida albicans
spellingShingle Determination of germ tube, phospholipase, and proteinase production by bloodstream isolates of Candida albicans
Mattei, Antonella Souza
Virulência
Fosfolipases
Peptídeo hidrolases
Candida albicans
Candidemia
Phospholipase
Candida albicans
Candidemia
Proteinase
Germ tube
Virulence
title_short Determination of germ tube, phospholipase, and proteinase production by bloodstream isolates of Candida albicans
title_full Determination of germ tube, phospholipase, and proteinase production by bloodstream isolates of Candida albicans
title_fullStr Determination of germ tube, phospholipase, and proteinase production by bloodstream isolates of Candida albicans
title_full_unstemmed Determination of germ tube, phospholipase, and proteinase production by bloodstream isolates of Candida albicans
title_sort Determination of germ tube, phospholipase, and proteinase production by bloodstream isolates of Candida albicans
dc.creator.none.fl_str_mv Mattei, Antonella Souza
Alves, Sydney Hartz
Severo, Cecília Bittencourt
Guazzelli, Luciana Silva
Oliveira, Flávio de Mattos
Severo, Luiz Carlos
author Mattei, Antonella Souza
author_facet Mattei, Antonella Souza
Alves, Sydney Hartz
Severo, Cecília Bittencourt
Guazzelli, Luciana Silva
Oliveira, Flávio de Mattos
Severo, Luiz Carlos
author_role author
author2 Alves, Sydney Hartz
Severo, Cecília Bittencourt
Guazzelli, Luciana Silva
Oliveira, Flávio de Mattos
Severo, Luiz Carlos
author2_role author
author
author
author
author
dc.subject.por.fl_str_mv Virulência
Fosfolipases
Peptídeo hidrolases
Candida albicans
Candidemia
topic Virulência
Fosfolipases
Peptídeo hidrolases
Candida albicans
Candidemia
Phospholipase
Candida albicans
Candidemia
Proteinase
Germ tube
Virulence
dc.subject.eng.fl_str_mv Phospholipase
Candida albicans
Candidemia
Proteinase
Germ tube
Virulence
description Candida albicans is a commensal and opportunistic agent that causes infection in immunocompromised individuals. Several attributes contribute to the virulence and pathogenicity of this yeast, including the production of germ tubes (GTs) and extracellular hydrolytic enzymes, particularly phospholipase and proteinase. This study aimed to investigate GT production and phospholipase and proteinase activities in bloodstream isolates of C. albicans. Methods: One hundred fi fty-three C. albicans isolates were obtained from blood samples and analyzed for GT, phospholipase, and proteinase production. The assays were performed in duplicate in egg yolk medium containing bovine serum albumin and human serum. Results: Detectable amounts of proteinase were produced by 97% of the isolates, and 78% of the isolates produced phospholipase. GTs were produced by 95% of the isolates. A majority of the isolates exhibited low levels of phospholipase production and high levels of proteinase production. Conclusions: Bloodstream isolates of C. albicans produce virulence factors such as GT and hydrolytic enzymes that enable them to cause infection under favorable conditions.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-05-07T02:04:32Z
dc.type.driver.fl_str_mv Estrangeiro
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/94965
dc.identifier.issn.pt_BR.fl_str_mv 0037-8682
dc.identifier.nrb.pt_BR.fl_str_mv 000898157
identifier_str_mv 0037-8682
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url http://hdl.handle.net/10183/94965
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical. Brasilia. Vol. 46, n. 3 (maio/jun. 2013), p. 340-342.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRGS
instname:Universidade Federal do Rio Grande do Sul (UFRGS)
instacron:UFRGS
instname_str Universidade Federal do Rio Grande do Sul (UFRGS)
instacron_str UFRGS
institution UFRGS
reponame_str Repositório Institucional da UFRGS
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