UV Spectrophotometry Method Validation for Quantification of Paracetamol in Tablet Formulations: A Proposal of Experimental Activity for Instrumental Analysis

In the present article it is described the validation of a simple, precise, accurate, rapid and low-cost UV spectrophotometric method for quantification of paracetamol in tablets. The method was linear in the range between 6.4 - 9.6 μg mL-1, presenting a good correlation coefficient (r = 0.9984) and...

ver descrição completa

Detalhes bibliográficos
Autores: Goes Junior, Enock José Alves, Roeder, Jakson Santos, Oliveira, Kaiky Borges Lima, Gois, Lucas de Castro, Ferreira, Mateus Pereira, da Silva, Jonatas Gomes
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:Brasil
Recursos:Universidade Federal de Mato Grosso do Sul (UFMS)
Repositorio:Orbital - The Electronic Journal of Chemistry (Campo Grande)
Idioma:inglés
OAI Identifier:oai:periodicos.ufms.br:article/16037
Acesso em linha:https://periodicos.ufms.br/index.php/orbital/article/view/16037
Access Level:acceso abierto
Palavra-chave:paracetamol
teaching laboratories
UV spectrophotometry
validation studies
Descrição
Resumo:In the present article it is described the validation of a simple, precise, accurate, rapid and low-cost UV spectrophotometric method for quantification of paracetamol in tablets. The method was linear in the range between 6.4 - 9.6 μg mL-1, presenting a good correlation coefficient (r = 0.9984) and adequate limits of quantification (2.64 μg mL-1) and detection (0.87 μg mL-1). Precision and analysis showed low coefficient of variation (< 4.0 %) and a good average recovery percentual (99.22% - reference and 101.78% - generic) was obtained. The method was applied for paracetamol determination in two tablet formulations and these results are in good accordance with the declared values of manufacturer, at a 95% confidence level. The experimental activity is simple, showing it to be used in experimental activities on teaching laboratories improve student learning. DOI: http://dx.doi.org/10.17807/orbital.v10i7.1330