Hemodialysis water: occurrence of yeasts, Pseudomonas aeruginosa, and heterotrophic bacteria

During the summer and winter of 2003, 200 samples of water used for hemodialysis procedures collected from two hospitals A and B were analyzed. The water samples were collected from the following sites: P1 - from incoming public treated water, from city water mains (N=18 samples); P2 - from pre-trea...

Descripción completa

Detalles Bibliográficos
Autores: Simões, Marise, Pires, Maria de Fátima C.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2004
País:Brasil
Institución:Instituto Adolfo Lutz
Repositorio:Revista do Instituto Adolfo Lutz (Online)
Idioma:portugués
OAI Identifier:oai:ojs.periodicos.saude.sp.gov.br:article/34862
Acceso en línea:https://periodicos.saude.sp.gov.br/RIAL/article/view/34862
Access Level:acceso abierto
Palabra clave:water
hemodialysis
Pseudomonas aeruginosa
yeast
heterotrophic bacteria
disinfections
água
água, hemodiálise
leveduras
bactérias heterotróficas
desinfecção
Descripción
Sumario:During the summer and winter of 2003, 200 samples of water used for hemodialysis procedures collected from two hospitals A and B were analyzed. The water samples were collected from the following sites: P1 - from incoming public treated water, from city water mains (N=18 samples); P2 - from pre-treatment site (N=18); P3 - from reverse osmosis (OR) treatment site (N=18); P4 - from hemodialysis machine water entry site (N=28); P5 - pre-dialyzing solution (N=30); P6 - from post-dialyzing site with arterial and venous blood lines (N=17); P7- from loop (N=9); P8 - from carbon filter (N=11); P9 - from reuse C site (N=18); P10 – from reuse B site (N=18); and P11- from water storage tank for OR (N=11). The analyses were performed according to the methodology recommended in the Standard Methods for the Examination of Water andWastewater, 1995, by means of the following assays: pour plate technique for heterotrophic bacteria (BH) counting in R2A agar; filter membrane technique for Pseudomonas aeruginosa detection in Cetrimide agar; culture in Sabouraud agar for yeast; technique for fluoride and conductivity determination; determination of sulfate and nitrate performed according to “Normas Analíticas do Instituto Adolfo Lutz”(Instituto Adolfo Lutz Analytical Guidelines), 1985...