Identifying MicroRNAs and transcript targets in Jartropha seeds

MicroRNAs, or miRNAs, are endogenously encoded small RNAs that play a key role in diverse plant biological processes. Jatropha curcas L. has received significant attention as a potential oilseed crop for the production of renewable oil. Here, a sRNA library of mature seeds and three mRNA libraries f...

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Autores: Galli, Vanessa, Guzman, Frank, Oliveira, Luiz Felipe Valter de, Morais, Guilherme Loss de, Korbes, Ana Paula, Silva, Sérgio Delmar dos Anjos e, Margis-Pinheiro, Márcia, Margis, Rogerio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:Brasil
Institución:Universidade Federal do Rio Grande do Sul (UFRGS)
Repositorio:Repositório Institucional da UFRGS
Idioma:inglés
OAI Identifier:oai:www.lume.ufrgs.br:10183/115268
Acceso en línea:http://hdl.handle.net/10183/115268
Access Level:acceso abierto
Palabra clave:MicroRNAs
Jartropha seeds
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spelling 2015-04-14T01:57:42Z20141932-6203http://hdl.handle.net/10183/115268000920358MicroRNAs, or miRNAs, are endogenously encoded small RNAs that play a key role in diverse plant biological processes. Jatropha curcas L. has received significant attention as a potential oilseed crop for the production of renewable oil. Here, a sRNA library of mature seeds and three mRNA libraries from three different seed development stages were generated by deep sequencing to identify and characterize the miRNAs and pre-miRNAs of J. curcas. Computational analysis was used for the identification of 180 conserved miRNAs and 41 precursors (pre-miRNAs) as well as 16 novel pre-miRNAs. The predicted miRNA target genes are involved in a broad range of physiological functions, including cellular structure, nuclear function, translation, transport, hormone synthesis, defense, and lipid metabolism. Some pre-miRNA and miRNA targets vary in abundance between the three stages of seed development. A search for sequences that produce siRNA was performed, and the results indicated that J. curcas siRNAs play a role in nuclear functions, transport, catalytic processes and disease resistance. This study presents the first large scale identification of J. curcas miRNAs and their targets in mature seeds based on deep sequencing, and it contributes to a functional understanding of these miRNAs.application/pdfengPLoS ONE. San Francisco. Vol. 9, no. 2 (Feb. 2014), e83727, 10 p.MicroRNAsJartropha seedsIdentifying MicroRNAs and transcript targets in Jartropha seedsEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSGalli, VanessaGuzman, FrankOliveira, Luiz Felipe Valter deMorais, Guilherme Loss deKorbes, Ana PaulaSilva, Sérgio Delmar dos Anjos eMargis-Pinheiro, MárciaMargis, RogerioORIGINAL000920358.pdf000920358.pdfTexto completo (inglês)application/pdf691687http://www.lume.ufrgs.br/bitstream/10183/115268/1/000920358.pdfdd1de8eba92a4bdfdd8b0065c21d8545MD51TEXT000920358.pdf.txt000920358.pdf.txtExtracted Texttext/plain55984http://www.lume.ufrgs.br/bitstream/10183/115268/2/000920358.pdf.txt73dca85ec865850ff2ab817785947501MD52THUMBNAIL000920358.pdf.jpg000920358.pdf.jpgGenerated Thumbnailimage/jpeg1987http://www.lume.ufrgs.br/bitstream/10183/115268/3/000920358.pdf.jpgfd545d6dffbefd3739373ea130adb093MD5310183/1152682023-09-23 03:37:31.735761oai:www.lume.ufrgs.br:10183/115268Repositório InstitucionalPUBhttps://lume.ufrgs.br/oai/requestlume@ufrgs.bropendoar:2023-09-23T06:37:31Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Identifying MicroRNAs and transcript targets in Jartropha seeds
title Identifying MicroRNAs and transcript targets in Jartropha seeds
spellingShingle Identifying MicroRNAs and transcript targets in Jartropha seeds
Galli, Vanessa
MicroRNAs
Jartropha seeds
title_short Identifying MicroRNAs and transcript targets in Jartropha seeds
title_full Identifying MicroRNAs and transcript targets in Jartropha seeds
title_fullStr Identifying MicroRNAs and transcript targets in Jartropha seeds
title_full_unstemmed Identifying MicroRNAs and transcript targets in Jartropha seeds
title_sort Identifying MicroRNAs and transcript targets in Jartropha seeds
dc.creator.none.fl_str_mv Galli, Vanessa
Guzman, Frank
Oliveira, Luiz Felipe Valter de
Morais, Guilherme Loss de
Korbes, Ana Paula
Silva, Sérgio Delmar dos Anjos e
Margis-Pinheiro, Márcia
Margis, Rogerio
author Galli, Vanessa
author_facet Galli, Vanessa
Guzman, Frank
Oliveira, Luiz Felipe Valter de
Morais, Guilherme Loss de
Korbes, Ana Paula
Silva, Sérgio Delmar dos Anjos e
Margis-Pinheiro, Márcia
Margis, Rogerio
author_role author
author2 Guzman, Frank
Oliveira, Luiz Felipe Valter de
Morais, Guilherme Loss de
Korbes, Ana Paula
Silva, Sérgio Delmar dos Anjos e
Margis-Pinheiro, Márcia
Margis, Rogerio
author2_role author
author
author
author
author
author
author
dc.subject.por.fl_str_mv MicroRNAs
Jartropha seeds
topic MicroRNAs
Jartropha seeds
description MicroRNAs, or miRNAs, are endogenously encoded small RNAs that play a key role in diverse plant biological processes. Jatropha curcas L. has received significant attention as a potential oilseed crop for the production of renewable oil. Here, a sRNA library of mature seeds and three mRNA libraries from three different seed development stages were generated by deep sequencing to identify and characterize the miRNAs and pre-miRNAs of J. curcas. Computational analysis was used for the identification of 180 conserved miRNAs and 41 precursors (pre-miRNAs) as well as 16 novel pre-miRNAs. The predicted miRNA target genes are involved in a broad range of physiological functions, including cellular structure, nuclear function, translation, transport, hormone synthesis, defense, and lipid metabolism. Some pre-miRNA and miRNA targets vary in abundance between the three stages of seed development. A search for sequences that produce siRNA was performed, and the results indicated that J. curcas siRNAs play a role in nuclear functions, transport, catalytic processes and disease resistance. This study presents the first large scale identification of J. curcas miRNAs and their targets in mature seeds based on deep sequencing, and it contributes to a functional understanding of these miRNAs.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-04-14T01:57:42Z
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dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
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dc.relation.ispartof.pt_BR.fl_str_mv PLoS ONE. San Francisco. Vol. 9, no. 2 (Feb. 2014), e83727, 10 p.
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