Identificação de diterpenos clerodânicos em diferentes órgãos de Casearia sylvestris Swartz

Casearia sylvestris Swartz (Salicaceae) is a tree or shrub distributed widely in Brazil, where it is used in popular medicine. Several bioactive clerodane diterpenes typical of Casearia have been isolated from this species (e.g. casearins and casearvestrins). The main objective of this study was to...

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Detalles Bibliográficos
Autores: Carvalho, E. S. [UNESP], Santos, A. G. [UNESP], Cavalheiro, Alberto José [UNESP]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:portugués
OAI Identifier:oai:repositorio.unesp.br:11449/71139
Acceso en línea:http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/543
http://hdl.handle.net/11449/71139
Access Level:acceso abierto
Palabra clave:Casearia sylvestris
Chromatography
Clerodane diterpenes
Flacourtiaceae
Salicaceae
Casearia sylvestris extract
clerodane derivative
plant extract
unclassified drug
Brazil
Casearia
chromatography
diode
drug identification
drug isolation
flower
high performance liquid chromatography
nonhuman
phytochemistry
plant leaf
plant root
plant stem
proton nuclear magnetic resonance
spectroscopy
thin layer chromatography
willow
Descripción
Sumario:Casearia sylvestris Swartz (Salicaceae) is a tree or shrub distributed widely in Brazil, where it is used in popular medicine. Several bioactive clerodane diterpenes typical of Casearia have been isolated from this species (e.g. casearins and casearvestrins). The main objective of this study was to identify clerodane diterpenes in various organs of C. sylvestris, using chromatographic and spectroscopic analytical techniques. The extracts of the different plant parts were analyzed by thin layer chromatography, high performance liquid chromatography with diode array detector and 1H nuclear magnetic resonance. In the chromatographic analysis, clerodane diterpenes isolated from C. sylvestris were used as standards, including rel-19Sacetóxi-18R- butanoilóxi-18,19- epóxi -6S -hidróxi -2R-(2-metilbutanoilóxi) -5S, 8R, 9R, 10S -cleroda-3,13(16),14-triene, isolated for the first time from the stems. Phytochemical profiles of the organs were produced, which indicated the presence of clerodane diterpenes in all parts of the plant, notably in the leaves. The results also suggest that the main clerodane diterpenes in the stems, flowers and roots had conjugated double-bond patterns that differed from those found in the leaves.