The efficiency of Reasoner’2 Agar for counting heterotrophic bacteria isolated from water for dialysis

To detect the potential risk which may interfere on the quality of the water used for dialysis procedure, some assessment parameters have been established led to the definition of for its evaluation, such as the counting of heterotrophic bacteria. Considering that the chosen culture media and the in...

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Autores: Almódovar, Adriana Aparecida Buzzo, Pereira, Tatiana Caldas, Bugno, Adriana
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:Brasil
Recursos:Instituto Adolfo Lutz
Repositorio:Revista do Instituto Adolfo Lutz (Online)
Idioma:portugués
OAI Identifier:oai:ojs.periodicos.saude.sp.gov.br:article/32722
Acesso em linha:https://periodicos.saude.sp.gov.br/RIAL/article/view/32722
Access Level:acceso abierto
Palavra-chave:water for dialysis
heterotrophic bacteria
Reasoner’2 agar
Plate Count Agar
água para diálise
bactérias heterotróficas
ágar R2A
ágar PCA
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spelling The efficiency of Reasoner’2 Agar for counting heterotrophic bacteria isolated from water for dialysisEficiência do Agar R2A na contagem de bactérias heterotróficas em água tratada para diálisewater for dialysisheterotrophic bacteriaReasoner’2 agarPlate Count Agarágua para diálisebactérias heterotróficaságar R2Aágar PCATo detect the potential risk which may interfere on the quality of the water used for dialysis procedure, some assessment parameters have been established led to the definition of for its evaluation, such as the counting of heterotrophic bacteria. Considering that the chosen culture media and the incubation conditions may interfere on the results from microbiological analysis on water for dialysis, the present study evaluated the efficiency of R2A culture medium for performing the heterotrophic bacteria counting. A total of 193 samples of water collected from dialysis clinics located in the city of São Paulo and in other cities of the Grande São Paulo region were analyzed, and also the incubation temperature and the efficiency comparison on bothR2A and PCA media were evaluated. No difference between bacteria counting on R2A medium after 96hincubationat 24oC and 34oC was found. The highest bacteria counting was found on R2A medium (p <0.02), which was considered to be mostly suitable for evaluating the water for dialysis. In order to minimize the risks to the patient under dialysis, the water for dialysis should be carried out by bacteria counting technique on R2A agar because of its high sensitivity, and incubating it for longer incubation times.Com o reconhecimento da má qualidade da água em causar potencial risco aos pacientes em tratamentos dialíticos, foram definidos os parâmetros de avaliação, entre os quais a contagem de bactérias heterotróficas. Considerando que a escolha dos meios de cultura e as condições de incubação utilizadas podem influenciar nos resultados da avaliação microbiológica da água tratada para diálise, este estudo realizou a análise da eficiência do ágar R2A para efetuar a contagem de bactérias heterotróficas. Foram analisadas 193amostras de água tratada provenientes de clínicas de diálise do município de São Paulo e de Grande São Paulo, em que foram avaliadas diferentes temperaturas de incubação, bem como o desempenho analítico de ágar R2A em comparação ao teste realizado com ágar PCA. Não houve diferença significativa entre as contagens obtidas após a incubação por 96 horas a temperaturas de 23oC e 34oC. Entretanto, contagens signifi cativamente maiores foram observadas em ágar R2A (p < 0,02), sendo este, portanto, mais adequado para a avaliação da qualidade de água tratada para diálise. Com a finalidade de minimizar os riscos ao paciente sob tratamento dialítico, recomenda-se o uso do teste em ágar R2A combinado com maior tempo de incubação, em função de sua maior sensibilidade, para a efetuar a contagem de bactérias heterotróficas em água tratada para diálise.Instituto Adolfo Lutz2009-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado pelos paresapplication/pdfhttps://periodicos.saude.sp.gov.br/RIAL/article/view/3272210.53393/rial.2009.v68.32722Instituto Adolfo Lutz Journal - RIAL; Vol. 68 No. 2 (2009); 232-236Revista del Instituto Adolfo Lutz - RIAL; Vol. 68 Núm. 2 (2009); 232-236Revista do Instituto Adolfo Lutz; v. 68 n. 2 (2009); 232-2361983-38140073-9855reponame:Revista do Instituto Adolfo Lutz (Online)instname:Instituto Adolfo Lutzinstacron:IALporhttps://periodicos.saude.sp.gov.br/RIAL/article/view/32722/31553Copyright (c) 2009 Adriana Aparecida Buzzo Almódovar, Tatiana Caldas Pereira, Adriana Bugnoinfo:eu-repo/semantics/openAccessAlmódovar, Adriana Aparecida BuzzoPereira, Tatiana CaldasBugno, Adriana2023-11-07T19:36:34Zoai:ojs.periodicos.saude.sp.gov.br:article/32722Revistahttps://periodicos.saude.sp.gov.br/RIAL/indexPUBhttps://periodicos.saude.sp.gov.br/RIAL/oairial@saude.sp.gov.brhttps://doi.org/10.53393/rial1983-38140073-9855opendoar:2023-11-07T19:36:34Revista do Instituto Adolfo Lutz (Online) - Instituto Adolfo Lutzfalse
dc.title.none.fl_str_mv The efficiency of Reasoner’2 Agar for counting heterotrophic bacteria isolated from water for dialysis
Eficiência do Agar R2A na contagem de bactérias heterotróficas em água tratada para diálise
title The efficiency of Reasoner’2 Agar for counting heterotrophic bacteria isolated from water for dialysis
spellingShingle The efficiency of Reasoner’2 Agar for counting heterotrophic bacteria isolated from water for dialysis
Almódovar, Adriana Aparecida Buzzo
water for dialysis
heterotrophic bacteria
Reasoner’2 agar
Plate Count Agar
água para diálise
bactérias heterotróficas
ágar R2A
ágar PCA
title_short The efficiency of Reasoner’2 Agar for counting heterotrophic bacteria isolated from water for dialysis
title_full The efficiency of Reasoner’2 Agar for counting heterotrophic bacteria isolated from water for dialysis
title_fullStr The efficiency of Reasoner’2 Agar for counting heterotrophic bacteria isolated from water for dialysis
title_full_unstemmed The efficiency of Reasoner’2 Agar for counting heterotrophic bacteria isolated from water for dialysis
title_sort The efficiency of Reasoner’2 Agar for counting heterotrophic bacteria isolated from water for dialysis
dc.creator.none.fl_str_mv Almódovar, Adriana Aparecida Buzzo
Pereira, Tatiana Caldas
Bugno, Adriana
author Almódovar, Adriana Aparecida Buzzo
author_facet Almódovar, Adriana Aparecida Buzzo
Pereira, Tatiana Caldas
Bugno, Adriana
author_role author
author2 Pereira, Tatiana Caldas
Bugno, Adriana
author2_role author
author
dc.subject.por.fl_str_mv water for dialysis
heterotrophic bacteria
Reasoner’2 agar
Plate Count Agar
água para diálise
bactérias heterotróficas
ágar R2A
ágar PCA
topic water for dialysis
heterotrophic bacteria
Reasoner’2 agar
Plate Count Agar
água para diálise
bactérias heterotróficas
ágar R2A
ágar PCA
description To detect the potential risk which may interfere on the quality of the water used for dialysis procedure, some assessment parameters have been established led to the definition of for its evaluation, such as the counting of heterotrophic bacteria. Considering that the chosen culture media and the incubation conditions may interfere on the results from microbiological analysis on water for dialysis, the present study evaluated the efficiency of R2A culture medium for performing the heterotrophic bacteria counting. A total of 193 samples of water collected from dialysis clinics located in the city of São Paulo and in other cities of the Grande São Paulo region were analyzed, and also the incubation temperature and the efficiency comparison on bothR2A and PCA media were evaluated. No difference between bacteria counting on R2A medium after 96hincubationat 24oC and 34oC was found. The highest bacteria counting was found on R2A medium (p <0.02), which was considered to be mostly suitable for evaluating the water for dialysis. In order to minimize the risks to the patient under dialysis, the water for dialysis should be carried out by bacteria counting technique on R2A agar because of its high sensitivity, and incubating it for longer incubation times.
publishDate 2009
dc.date.none.fl_str_mv 2009-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.saude.sp.gov.br/RIAL/article/view/32722
10.53393/rial.2009.v68.32722
url https://periodicos.saude.sp.gov.br/RIAL/article/view/32722
identifier_str_mv 10.53393/rial.2009.v68.32722
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.saude.sp.gov.br/RIAL/article/view/32722/31553
dc.rights.driver.fl_str_mv Copyright (c) 2009 Adriana Aparecida Buzzo Almódovar, Tatiana Caldas Pereira, Adriana Bugno
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2009 Adriana Aparecida Buzzo Almódovar, Tatiana Caldas Pereira, Adriana Bugno
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Adolfo Lutz
publisher.none.fl_str_mv Instituto Adolfo Lutz
dc.source.none.fl_str_mv Instituto Adolfo Lutz Journal - RIAL; Vol. 68 No. 2 (2009); 232-236
Revista del Instituto Adolfo Lutz - RIAL; Vol. 68 Núm. 2 (2009); 232-236
Revista do Instituto Adolfo Lutz; v. 68 n. 2 (2009); 232-236
1983-3814
0073-9855
reponame:Revista do Instituto Adolfo Lutz (Online)
instname:Instituto Adolfo Lutz
instacron:IAL
instname_str Instituto Adolfo Lutz
instacron_str IAL
institution IAL
reponame_str Revista do Instituto Adolfo Lutz (Online)
collection Revista do Instituto Adolfo Lutz (Online)
repository.name.fl_str_mv Revista do Instituto Adolfo Lutz (Online) - Instituto Adolfo Lutz
repository.mail.fl_str_mv rial@saude.sp.gov.br
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score 15,228081

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