Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil

Braga, Maria do Socorro Costa de Oliveira; Costa, Francisca Neide; Gomes, Débora Regina Maia; Xavier, Daniele Rosa; André, Marcos Rogério [UNESP]; Gonçalves, Luiz Ricardo [UNESP]; Freschi, Carla Roberta [UNESP]; Machado, Rosangela Zacarias [UNESP]

Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil

Equine piroplasmosisis, a tick-borne disease caused by the intra-erythrocytic protozoans Babesia caballi and Theileria equi, has economic importance due to the international trade and the increased movement of horses all over the world. The goal of this study was to evaluate the occurrence of phylog...

Descripción completa

Detalles Bibliográficos
Autores:	Braga, Maria do Socorro Costa de Oliveira, Costa, Francisca Neide, Gomes, Débora Regina Maia, Xavier, Daniele Rosa, André, Marcos Rogério [UNESP], Gonçalves, Luiz Ricardo [UNESP], Freschi, Carla Roberta [UNESP], Machado, Rosangela Zacarias [UNESP]
Tipo de recurso:	artículo
Estado:	Versión publicada
Fecha de publicación:	2017
País:	Brasil
Institución:	Universidade Estadual Paulista (UNESP)
Repositorio:	Repositório Institucional da UNESP
Idioma:	inglés
OAI Identifier:	oai:repositorio.unesp.br:11449/175299
Acceso en línea:	http://dx.doi.org/10.1590/S1984-29612017046 http://hdl.handle.net/11449/175299
Access Level:	acceso abierto
Palabra clave:	18S rRNA Babesia caballi Phylogeny Theileria equi

id	BR_bbb7fe3d8d832ec828e657382e36cd02
oai_identifier_str	oai:repositorio.unesp.br:11449/175299
network_acronym_str	BR
network_name_str	Brasil
repository_id_str
spelling	Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern BrazilDiversidade genética de espécies de piroplasmídeos em equídeos na ilha de São Luís, Nordeste do Brasil18S rRNABabesia caballiPhylogenyTheileria equiEquine piroplasmosisis, a tick-borne disease caused by the intra-erythrocytic protozoans Babesia caballi and Theileria equi, has economic importance due to the international trade and the increased movement of horses all over the world. The goal of this study was to evaluate the occurrence of phylogenetic diversity of T. equi and B. caballi genotypes among infected equids from São Luís Island, state of Maranhão, northeastern Brazil. Between December of 2011 and June of 2012, EDTA-blood and serum samples were collected from 139 equids (90 donkeys, 39 horses and 10 mules). From 139 serum samples submitted to ELISA assay, IgG antibodies to T. equi and B. caballi were detected in 19.4% (27/139) and 25.2% (35/139), respectively. Among sampled animals, 21.6% (30/139) and 55.4% (77/139) were positive for cPCR assays for T. equi and B. caballi, based on ema-1 and rap-1 genes, respectively. Overall, the T. equi sequences (n=7) submitted to Maximum Likelihood analysis (based on a 18S rRNA fragment of 1700 bp after alignment) grouped into three main groups, which were subdivided in eight clusters. The present work showed that different genotypes of T. equi and B. caballi circulate among equids in Brazil.Universidade Estadual do Maranhão-UEMALaboratório de Imunoparasitologia Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias-FCAV Universidade Estadual Paulista-UNESPLaboratório de Imunoparasitologia Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias-FCAV Universidade Estadual Paulista-UNESPUniversidade Estadual de Maringá (UEM)Universidade Estadual Paulista (Unesp)2018-12-11T17:15:12Z2018-12-11T17:15:12Z2017-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article331-339application/pdfhttp://dx.doi.org/10.1590/S1984-29612017046Revista Brasileira de Parasitologia Veterinaria, v. 26, n. 3, p. 331-339, 2017.0103-846Xhttp://hdl.handle.net/11449/17529910.1590/S1984-29612017046S1984-296120170003003312-s2.0-85030669895S1984-29612017000300331.pdf9139899895580513Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Parasitologia Veterinariainfo:eu-repo/semantics/openAccessBraga, Maria do Socorro Costa de OliveiraCosta, Francisca NeideGomes, Débora Regina MaiaXavier, Daniele RosaAndré, Marcos Rogério [UNESP]Gonçalves, Luiz Ricardo [UNESP]Freschi, Carla Roberta [UNESP]Machado, Rosangela Zacarias [UNESP]2024-06-07T13:02:38Zoai:repositorio.unesp.br:11449/175299Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-06-07T13:02:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv	Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil Diversidade genética de espécies de piroplasmídeos em equídeos na ilha de São Luís, Nordeste do Brasil
title	Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil
spellingShingle	Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil Braga, Maria do Socorro Costa de Oliveira 18S rRNA Babesia caballi Phylogeny Theileria equi
title_short	Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil
title_full	Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil
title_fullStr	Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil
title_full_unstemmed	Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil
title_sort	Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil
dc.creator.none.fl_str_mv	Braga, Maria do Socorro Costa de Oliveira Costa, Francisca Neide Gomes, Débora Regina Maia Xavier, Daniele Rosa André, Marcos Rogério [UNESP] Gonçalves, Luiz Ricardo [UNESP] Freschi, Carla Roberta [UNESP] Machado, Rosangela Zacarias [UNESP]
author	Braga, Maria do Socorro Costa de Oliveira
author_facet	Braga, Maria do Socorro Costa de Oliveira Costa, Francisca Neide Gomes, Débora Regina Maia Xavier, Daniele Rosa André, Marcos Rogério [UNESP] Gonçalves, Luiz Ricardo [UNESP] Freschi, Carla Roberta [UNESP] Machado, Rosangela Zacarias [UNESP]
author_role	author
author2	Costa, Francisca Neide Gomes, Débora Regina Maia Xavier, Daniele Rosa André, Marcos Rogério [UNESP] Gonçalves, Luiz Ricardo [UNESP] Freschi, Carla Roberta [UNESP] Machado, Rosangela Zacarias [UNESP]
author2_role	author author author author author author author
dc.contributor.none.fl_str_mv	Universidade Estadual de Maringá (UEM) Universidade Estadual Paulista (Unesp)
dc.subject.por.fl_str_mv	18S rRNA Babesia caballi Phylogeny Theileria equi
topic	18S rRNA Babesia caballi Phylogeny Theileria equi
description	Equine piroplasmosisis, a tick-borne disease caused by the intra-erythrocytic protozoans Babesia caballi and Theileria equi, has economic importance due to the international trade and the increased movement of horses all over the world. The goal of this study was to evaluate the occurrence of phylogenetic diversity of T. equi and B. caballi genotypes among infected equids from São Luís Island, state of Maranhão, northeastern Brazil. Between December of 2011 and June of 2012, EDTA-blood and serum samples were collected from 139 equids (90 donkeys, 39 horses and 10 mules). From 139 serum samples submitted to ELISA assay, IgG antibodies to T. equi and B. caballi were detected in 19.4% (27/139) and 25.2% (35/139), respectively. Among sampled animals, 21.6% (30/139) and 55.4% (77/139) were positive for cPCR assays for T. equi and B. caballi, based on ema-1 and rap-1 genes, respectively. Overall, the T. equi sequences (n=7) submitted to Maximum Likelihood analysis (based on a 18S rRNA fragment of 1700 bp after alignment) grouped into three main groups, which were subdivided in eight clusters. The present work showed that different genotypes of T. equi and B. caballi circulate among equids in Brazil.
publishDate	2017
dc.date.none.fl_str_mv	2017-07-01 2018-12-11T17:15:12Z 2018-12-11T17:15:12Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.uri.fl_str_mv	http://dx.doi.org/10.1590/S1984-29612017046 Revista Brasileira de Parasitologia Veterinaria, v. 26, n. 3, p. 331-339, 2017. 0103-846X http://hdl.handle.net/11449/175299 10.1590/S1984-29612017046 S1984-29612017000300331 2-s2.0-85030669895 S1984-29612017000300331.pdf 9139899895580513
url	http://dx.doi.org/10.1590/S1984-29612017046 http://hdl.handle.net/11449/175299
identifier_str_mv	Revista Brasileira de Parasitologia Veterinaria, v. 26, n. 3, p. 331-339, 2017. 0103-846X 10.1590/S1984-29612017046 S1984-29612017000300331 2-s2.0-85030669895 S1984-29612017000300331.pdf 9139899895580513
dc.language.iso.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	Revista Brasileira de Parasitologia Veterinaria
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	331-339 application/pdf
dc.source.none.fl_str_mv	Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP
instname_str	Universidade Estadual Paulista (UNESP)
instacron_str	UNESP
institution	UNESP
reponame_str	Repositório Institucional da UNESP
collection	Repositório Institucional da UNESP
repository.name.fl_str_mv	Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv	repositoriounesp@unesp.br
_version_	1853672097588445184
score	15,198674

Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil

Similares en LA Referencia