Identification and effects of mixed infection of Potyvirus isolates with Cucumber mosaic virus in cucurbits.

Mixed infections in cucurbits are frequently observed in natural conditions between viruses from the Potyvirus genus and Cucumber mosaic virus (CMV), which significantly decreases productivity. The objectives of the present study was to compare the host range of PRSV-W, WMV, and ZYMV isolates and ev...

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Detalles Bibliográficos
Autores: BARBOSA, G. da S., LIMA, J. A. de A., QUEIROZ, M. A. de, DIAS, R. de C. S., LIMA, C. S.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:Brasil
Institución:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
Repositorio:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Idioma:inglés
OAI Identifier:oai:www.alice.cnptia.embrapa.br:doc/1053338
Acceso en línea:http://www.alice.cnptia.embrapa.br/alice/handle/doc/1053338
Access Level:acceso abierto
Palabra clave:Mosaico do mamoeiro
Vírus do mosaico da melancia
Cucurbitácea
Doença
Virus
Papaya ringspot virus
Zucchini yellow mosaic virus
Watermelon mosaic virus
Cucumber mosaic virus
Potyvirus
Descripción
Sumario:Mixed infections in cucurbits are frequently observed in natural conditions between viruses from the Potyvirus genus and Cucumber mosaic virus (CMV), which significantly decreases productivity. The objectives of the present study was to compare the host range of PRSV-W, WMV, and ZYMV isolates and evaluate the effects of mixed infections with CMV in zucchini plants (Cucurbita pepo L.). Host range studies comprising 23 plant species confirmed some similarities and biological differences among the isolates of PRSV-W, ZYMV, and WMV. RT-PCR confirmed the amplification of DNA fragments of the PRSV-W, WMV, and ZYMV coat protein gene (cp) and cytoplasm inclusion gene (ci). The virus interaction studies in zucchini Caserta plants indicated synergistic interactions, particularly among species from the Potyvirus genus, and some CMV interference with some virus combinations.