Molecular detection and characterization of vector-borne agents in common opossums (Didelphis marsupialis) from northeastern Brazil
Opossums are synanthropic marsupials able to interchange among wild, peri‑urban and urban environments, playing an epidemiologically important role as hosts for emerging pathogens and ectoparasites of relevance in public health. The present study aimed to detect and molecularly characterize vector-b...
| Autores: | , , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | Brasil |
| Institución: | Universidade Estadual Paulista (UNESP) |
| Repositorio: | Repositório Institucional da UNESP |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unesp.br:11449/298183 |
| Acceso en línea: | http://dx.doi.org/10.1016/j.actatropica.2023.106955 https://hdl.handle.net/11449/298183 |
| Access Level: | acceso abierto |
| Palabra clave: | Babesia spp. Bartonella spp. Ehrlichia spp. Hemoplasmas Hepatozoon spp. Opossum |
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BR_b01d83669dcc4dc4e8f10df9d8aed008 |
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oai:repositorio.unesp.br:11449/298183 |
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BR |
| network_name_str |
Brasil |
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|
| dc.title.none.fl_str_mv |
Molecular detection and characterization of vector-borne agents in common opossums (Didelphis marsupialis) from northeastern Brazil |
| title |
Molecular detection and characterization of vector-borne agents in common opossums (Didelphis marsupialis) from northeastern Brazil |
| spellingShingle |
Molecular detection and characterization of vector-borne agents in common opossums (Didelphis marsupialis) from northeastern Brazil Braga, Maria do Socorro Costa Oliveira Babesia spp. Bartonella spp. Ehrlichia spp. Hemoplasmas Hepatozoon spp. Opossum |
| title_short |
Molecular detection and characterization of vector-borne agents in common opossums (Didelphis marsupialis) from northeastern Brazil |
| title_full |
Molecular detection and characterization of vector-borne agents in common opossums (Didelphis marsupialis) from northeastern Brazil |
| title_fullStr |
Molecular detection and characterization of vector-borne agents in common opossums (Didelphis marsupialis) from northeastern Brazil |
| title_full_unstemmed |
Molecular detection and characterization of vector-borne agents in common opossums (Didelphis marsupialis) from northeastern Brazil |
| title_sort |
Molecular detection and characterization of vector-borne agents in common opossums (Didelphis marsupialis) from northeastern Brazil |
| dc.creator.none.fl_str_mv |
Braga, Maria do Socorro Costa Oliveira Costa, Francisco Borges Calchi, Ana Cláudia [UNESP] de Mello, Victória Valente Califre [UNESP] Mongruel, Anna Claudia Baumel [UNESP] Dias, Clara Morato [UNESP] Bassini-Silva, Ricardo [UNESP] Silva, Ellainy Maria Conceição Pereira, José Gomes Ribeiro, Larissa Sarmento dos Santos da Costa, Andréa Pereira de Andrade, Fabio Henrique Evangelista Silva, Ana Lucia Abreu Machado, Rosangela Zacarias [UNESP] André, Marcos Rogério [UNESP] |
| author |
Braga, Maria do Socorro Costa Oliveira |
| author_facet |
Braga, Maria do Socorro Costa Oliveira Costa, Francisco Borges Calchi, Ana Cláudia [UNESP] de Mello, Victória Valente Califre [UNESP] Mongruel, Anna Claudia Baumel [UNESP] Dias, Clara Morato [UNESP] Bassini-Silva, Ricardo [UNESP] Silva, Ellainy Maria Conceição Pereira, José Gomes Ribeiro, Larissa Sarmento dos Santos da Costa, Andréa Pereira de Andrade, Fabio Henrique Evangelista Silva, Ana Lucia Abreu Machado, Rosangela Zacarias [UNESP] André, Marcos Rogério [UNESP] |
| author_role |
author |
| author2 |
Costa, Francisco Borges Calchi, Ana Cláudia [UNESP] de Mello, Victória Valente Califre [UNESP] Mongruel, Anna Claudia Baumel [UNESP] Dias, Clara Morato [UNESP] Bassini-Silva, Ricardo [UNESP] Silva, Ellainy Maria Conceição Pereira, José Gomes Ribeiro, Larissa Sarmento dos Santos da Costa, Andréa Pereira de Andrade, Fabio Henrique Evangelista Silva, Ana Lucia Abreu Machado, Rosangela Zacarias [UNESP] André, Marcos Rogério [UNESP] |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Estadual de Maringá (UEM) Universidade Estadual Paulista (UNESP) |
| dc.subject.por.fl_str_mv |
Babesia spp. Bartonella spp. Ehrlichia spp. Hemoplasmas Hepatozoon spp. Opossum |
| topic |
Babesia spp. Bartonella spp. Ehrlichia spp. Hemoplasmas Hepatozoon spp. Opossum |
| description |
Opossums are synanthropic marsupials able to interchange among wild, peri‑urban and urban environments, playing an epidemiologically important role as hosts for emerging pathogens and ectoparasites of relevance in public health. The present study aimed to detect and molecularly characterize vector-borne agents in a population of common opossums (Didelphis marsupialis) from the Island of São Luís do Maranhão, northeastern Brazil. Of the 45 animals analyzed, one (2.22%) was positive in the nested PCR assay based on the 18S rRNA gene of piroplasmids. The obtained sequence was phylogenetically positioned in a clade containing sequences of Babesia sp. previously detected in Didelphis aurita, Didelphis albiventris and associated ticks from Brazil. Eight (17.77%) samples were positive in PCR for Ehrlichia spp. based on the dsb gene; four samples were sequenced and positioned into a new clade, sister to E. minasensis and Ehrlichia sp. clade detected in Superorder Xenarthra mammals. No samples tested positive in the screening PCR assays based on the 16S rRNA gene of Anaplasma spp. Two samples were positive in the qPCR for Bartonella spp. based on the nuoG gene. Seven animals (15.56%) were positive in the nPCR based on the 16S rRNA gene of hemoplasmas. Of these, three were positive in a PCR based on the 23S rRNA gene. The phylogenies based on both 16S rRNA and 23S rRNA genes corroborated to each other and positioned the sequences in the same clade of hemoplasmas previously detected in D. aurita and D. albiventris sampled in Brazil. Finally, three (6.66%) animals were positive in the PCR for Hepatozoon spp.; the obtained 18S rRNA sequence was positioned into the H. felis clade.The present study showed, for the first time, the circulation of piroplasmids, Hepatozoon spp., Ehrlichia spp., hemoplasmas and Bartonella spp. in D. marsupialis sampled in northeastern Brazil, with description of putative novel genotypes of Ehrlichia and Hepatozoon and copositivity by different vector-borne agents. The present work consolidates the South American Marsupialia piroplasmid clade, adding one more genotype of Babesia sp. to this clade. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-08-01 2025-04-29T18:36:23Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.actatropica.2023.106955 Acta Tropica, v. 244. 1873-6254 0001-706X https://hdl.handle.net/11449/298183 10.1016/j.actatropica.2023.106955 2-s2.0-85162775688 |
| url |
http://dx.doi.org/10.1016/j.actatropica.2023.106955 https://hdl.handle.net/11449/298183 |
| identifier_str_mv |
Acta Tropica, v. 244. 1873-6254 0001-706X 10.1016/j.actatropica.2023.106955 2-s2.0-85162775688 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Acta Tropica |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
| instname_str |
Universidade Estadual Paulista (UNESP) |
| instacron_str |
UNESP |
| institution |
UNESP |
| reponame_str |
Repositório Institucional da UNESP |
| collection |
Repositório Institucional da UNESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
| repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
| _version_ |
1853672247721459712 |
| spelling |
Molecular detection and characterization of vector-borne agents in common opossums (Didelphis marsupialis) from northeastern BrazilBabesia spp.Bartonella spp.Ehrlichia spp.HemoplasmasHepatozoon spp.OpossumOpossums are synanthropic marsupials able to interchange among wild, peri‑urban and urban environments, playing an epidemiologically important role as hosts for emerging pathogens and ectoparasites of relevance in public health. The present study aimed to detect and molecularly characterize vector-borne agents in a population of common opossums (Didelphis marsupialis) from the Island of São Luís do Maranhão, northeastern Brazil. Of the 45 animals analyzed, one (2.22%) was positive in the nested PCR assay based on the 18S rRNA gene of piroplasmids. The obtained sequence was phylogenetically positioned in a clade containing sequences of Babesia sp. previously detected in Didelphis aurita, Didelphis albiventris and associated ticks from Brazil. Eight (17.77%) samples were positive in PCR for Ehrlichia spp. based on the dsb gene; four samples were sequenced and positioned into a new clade, sister to E. minasensis and Ehrlichia sp. clade detected in Superorder Xenarthra mammals. No samples tested positive in the screening PCR assays based on the 16S rRNA gene of Anaplasma spp. Two samples were positive in the qPCR for Bartonella spp. based on the nuoG gene. Seven animals (15.56%) were positive in the nPCR based on the 16S rRNA gene of hemoplasmas. Of these, three were positive in a PCR based on the 23S rRNA gene. The phylogenies based on both 16S rRNA and 23S rRNA genes corroborated to each other and positioned the sequences in the same clade of hemoplasmas previously detected in D. aurita and D. albiventris sampled in Brazil. Finally, three (6.66%) animals were positive in the PCR for Hepatozoon spp.; the obtained 18S rRNA sequence was positioned into the H. felis clade.The present study showed, for the first time, the circulation of piroplasmids, Hepatozoon spp., Ehrlichia spp., hemoplasmas and Bartonella spp. in D. marsupialis sampled in northeastern Brazil, with description of putative novel genotypes of Ehrlichia and Hepatozoon and copositivity by different vector-borne agents. The present work consolidates the South American Marsupialia piroplasmid clade, adding one more genotype of Babesia sp. to this clade.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual do Maranhão (UEMA), MAVector-Borne Bioagents Laboratory (VBBL) Department of Pathology Reproduction and One Health School of Agricultural and Veterinarian Sciences (FCAV) São Paulo State University (Unesp), SPVector-Borne Bioagents Laboratory (VBBL) Department of Pathology Reproduction and One Health School of Agricultural and Veterinarian Sciences (FCAV) São Paulo State University (Unesp), SPFAPESP: #2020/12037-0CNPq: #303701/2021-8FAPESP: 2019/24403-0FAPESP: 2020/07826-5FAPESP: 2020/11755-6Universidade Estadual de Maringá (UEM)Universidade Estadual Paulista (UNESP)2025-04-29T18:36:23Z2023-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.actatropica.2023.106955Acta Tropica, v. 244.1873-62540001-706Xhttps://hdl.handle.net/11449/29818310.1016/j.actatropica.2023.1069552-s2.0-85162775688Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Tropicainfo:eu-repo/semantics/openAccessBraga, Maria do Socorro Costa OliveiraCosta, Francisco BorgesCalchi, Ana Cláudia [UNESP]de Mello, Victória Valente Califre [UNESP]Mongruel, Anna Claudia Baumel [UNESP]Dias, Clara Morato [UNESP]Bassini-Silva, Ricardo [UNESP]Silva, Ellainy Maria ConceiçãoPereira, José GomesRibeiro, Larissa Sarmento dos Santosda Costa, Andréa Pereirade Andrade, Fabio Henrique EvangelistaSilva, Ana Lucia AbreuMachado, Rosangela Zacarias [UNESP]André, Marcos Rogério [UNESP]2025-10-22T13:09:30Zoai:repositorio.unesp.br:11449/298183Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-10-22T13:09:30Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
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15,301629 |