Effect of low density lipoprotein on the quality of cryopreserved dog semen
Egg yolk is included in extenders for semen cryopreservation due to its protective effect against cold shock,which is attributed to the presence of low density lipoprotein (LDL). This study evaluates how semen quality is affected by using LDL as a replacement for egg yolk in extenders for cooled and...
| Autores: | , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2008 |
| País: | Brasil |
| Institución: | Universidade Federal do Rio Grande (FURG) |
| Repositorio: | Repositório Institucional da FURG (RI FURG) |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.furg.br:1/84 |
| Acceso en línea: | http://repositorio.furg.br/handle/1/84 |
| Access Level: | acceso abierto |
| Palabra clave: | Low density lipoprotein Semen extenders Cryopreservation Dog semen |
| Sumario: | Egg yolk is included in extenders for semen cryopreservation due to its protective effect against cold shock,which is attributed to the presence of low density lipoprotein (LDL). This study evaluates how semen quality is affected by using LDL as a replacement for egg yolk in extenders for cooled and frozen dog semen. In Experiment 1 semen was extended in TRIS–glucoseat5ºC, in four treatments: 20% egg yolk (T1); 6% (T2); 8% (T3); and 10% LDL (T4). Spermmotility and membrane integrity after 24, 48, 72 and 96 hand the 50% conservation rate of motil espermatozoa (50M) were evaluated. The 50 M was less for T1 than for the other treatments (P <0.01), but T2–T4 did not differ (P >0.05). In Experiment 2, glycerol at 10% was included in the freezing extender, in treatments similar to those from Experiment 1. Spermmotility and membrane integrity did not differ for T2, T3 and T4 at any period in Experiment 1 and after thawing in Experiment 2 (P >0.05), but were greater for all LDL treatments than for T1 (P <0.01), in both experiments. Thus, LDL can replace egg yolk in the composition of the TRIS–glucose extender for cooled or frozen dog semen. |
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