Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae)

his study was carried out to assess the antibacterial, cytotoxic and antioxidant activities of extracts of Morus nigra L. HPLC was used to determine the fingerprint chromatogram of the crude ethanolic extract (Mn-EtOH). The antibacterial effect was assessed through the method of microdilution. The c...

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Detalles Bibliográficos
Autores: Souza, G. R., Oliveira-Junior, Raimundo Gonçalves de, Diniz, Tamara Coimbra, Branco, ALexsandro, Lima-Saraiva, Sara Raquel G., Guimarães, A. L., Oliveira, Ana Paula, Pacheco, A. G. M., Silva, M. G., Moraes Filho, Manoel Odorico de, Costa, M. P., Pessoa, Cláudia Ó., Almeida, Jackson Roberto Guedes da Silva
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Brasil
Institución:Universidade Federal do Ceará (UFC)
Repositorio:Repositório Institucional da Universidade Federal do Ceará (UFC)
Idioma:inglés
OAI Identifier:oai:repositorio.ufc.br:riufc/25426
Acceso en línea:http://www.repositorio.ufc.br/handle/riufc/25426
Access Level:acceso abierto
Palabra clave:Antioxidantes
Moraceae
Morus
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network_acronym_str BR
network_name_str Brasil
repository_id_str
dc.title.pt_BR.fl_str_mv Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae)
title Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae)
spellingShingle Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae)
Souza, G. R.
Antioxidantes
Moraceae
Morus
title_short Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae)
title_full Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae)
title_fullStr Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae)
title_full_unstemmed Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae)
title_sort Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae)
dc.creator.none.fl_str_mv Souza, G. R.
Oliveira-Junior, Raimundo Gonçalves de
Diniz, Tamara Coimbra
Branco, ALexsandro
Lima-Saraiva, Sara Raquel G.
Guimarães, A. L.
Oliveira, Ana Paula
Pacheco, A. G. M.
Silva, M. G.
Moraes Filho, Manoel Odorico de
Costa, M. P.
Pessoa, Cláudia Ó.
Almeida, Jackson Roberto Guedes da Silva
author Souza, G. R.
author_facet Souza, G. R.
Oliveira-Junior, Raimundo Gonçalves de
Diniz, Tamara Coimbra
Branco, ALexsandro
Lima-Saraiva, Sara Raquel G.
Guimarães, A. L.
Oliveira, Ana Paula
Pacheco, A. G. M.
Silva, M. G.
Moraes Filho, Manoel Odorico de
Costa, M. P.
Pessoa, Cláudia Ó.
Almeida, Jackson Roberto Guedes da Silva
author_role author
author2 Oliveira-Junior, Raimundo Gonçalves de
Diniz, Tamara Coimbra
Branco, ALexsandro
Lima-Saraiva, Sara Raquel G.
Guimarães, A. L.
Oliveira, Ana Paula
Pacheco, A. G. M.
Silva, M. G.
Moraes Filho, Manoel Odorico de
Costa, M. P.
Pessoa, Cláudia Ó.
Almeida, Jackson Roberto Guedes da Silva
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.por.fl_str_mv Antioxidantes
Moraceae
Morus
topic Antioxidantes
Moraceae
Morus
description his study was carried out to assess the antibacterial, cytotoxic and antioxidant activities of extracts of Morus nigra L. HPLC was used to determine the fingerprint chromatogram of the crude ethanolic extract (Mn-EtOH). The antibacterial effect was assessed through the method of microdilution. The cytotoxicity was tested against human tumour cell lines using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The total phenolic and flavonoid contents were also assessed through the Folin-Ciocalteu and aluminum chloride methods, respectively. Antioxidant activities of the extracts were evaluated by using 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging and β-carotene-linoleic acid bleaching methods. The presence of phenolic compounds in Mn-EtOH was confirmed using HPLC. The extracts showed activity against most microorganisms tested. The extracts did not show any expressive antiproliferative effect in the assessment of cytotoxicity. The most significant total phenolic content was 153.00 ± 11.34 mg of gallic acid equivalent/g to the ethyl acetate extract (AcOEt). The total flavonoid content was 292.50 ± 70.34 mg of catechin equivalent/g to the AcOEt extract, which presented the best antioxidant activity (IC50 50.40 ± 1.16 μg/mL) for DPPH scavenging. We can conclude that this species shows strong antibacterial and antioxidant activities, as well as weak cytotoxic effects.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-09-04T16:48:53Z
dc.date.available.fl_str_mv 2017-09-04T16:48:53Z
dc.date.issued.fl_str_mv 2017-08
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUZA, G. R. et al. Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae). Brazilian Journal of Biology, São Paulo, v. --, p. 1-7, aug. 2017.
dc.identifier.uri.fl_str_mv http://www.repositorio.ufc.br/handle/riufc/25426
dc.identifier.issn.none.fl_str_mv 1678-4375
1519-6984
identifier_str_mv SOUZA, G. R. et al. Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae). Brazilian Journal of Biology, São Paulo, v. --, p. 1-7, aug. 2017.
1678-4375
1519-6984
url http://www.repositorio.ufc.br/handle/riufc/25426
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Brazilian Journal of Biology
publisher.none.fl_str_mv Brazilian Journal of Biology
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
bitstream.url.fl_str_mv http://repositorio.ufc.br/bitstream/riufc/25426/1/2017_art_grsouza.pdf
http://repositorio.ufc.br/bitstream/riufc/25426/2/license.txt
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bitstream.checksumAlgorithm.fl_str_mv MD5
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repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
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spelling 2017-09-04T16:48:53Z2017-09-04T16:48:53Z2017-08SOUZA, G. R. et al. Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae). Brazilian Journal of Biology, São Paulo, v. --, p. 1-7, aug. 2017.1678-43751519-6984http://www.repositorio.ufc.br/handle/riufc/25426his study was carried out to assess the antibacterial, cytotoxic and antioxidant activities of extracts of Morus nigra L. HPLC was used to determine the fingerprint chromatogram of the crude ethanolic extract (Mn-EtOH). The antibacterial effect was assessed through the method of microdilution. The cytotoxicity was tested against human tumour cell lines using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The total phenolic and flavonoid contents were also assessed through the Folin-Ciocalteu and aluminum chloride methods, respectively. Antioxidant activities of the extracts were evaluated by using 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging and β-carotene-linoleic acid bleaching methods. The presence of phenolic compounds in Mn-EtOH was confirmed using HPLC. The extracts showed activity against most microorganisms tested. The extracts did not show any expressive antiproliferative effect in the assessment of cytotoxicity. The most significant total phenolic content was 153.00 ± 11.34 mg of gallic acid equivalent/g to the ethyl acetate extract (AcOEt). The total flavonoid content was 292.50 ± 70.34 mg of catechin equivalent/g to the AcOEt extract, which presented the best antioxidant activity (IC50 50.40 ± 1.16 μg/mL) for DPPH scavenging. We can conclude that this species shows strong antibacterial and antioxidant activities, as well as weak cytotoxic effects.Este estudo foi realizado para avaliar as atividades antibacteriana, citotóxica e antioxidante de extratos de Morus nigra L. HPLC foi utilizado para determinar o perfil de compostos fenólicos do extrato etanólico bruto (Mn-EtOH). O efeito antibacteriano foi avaliado através do método de microdiluição. A citotoxicidade foi testada contra linhagens celulares de tumores humanos utilizando o ensaio do brometo de 3-(4,5-dimetil-2-tiazolil)-2,5-difenil-2H-tetrazólio (MTT). O conteúdo total de compostos fenólicos e flavonoides também foi avaliado por meio dos métodos de Folin-Ciocalteu e cloreto de alumínio, respectivamente. A atividade antioxidante dos extratos foi avaliada por meio do sequestro do radical livre 2,2-difenil-1-picrilhidrazil (DPPH) e co-oxidação do sistema β-caroteno-ácido linoleico. A presença de compostos fenólicos em Mn-EtOH foi confirmada utilizando HPLC. Os extratos mostraram atividade contra a maioria dos microrganismos testados. Os extratos não mostraram qualquer efeito antiproliferativo expressivo na avaliação da citotoxicidade. O conteúdo fenólico total mais significativo foi de 153,00 ± 11,34 mg de equivalente de ácido gálico/g para o extrato acetato de etila (AcOEt). O conteúdo de flavonoides totais foi de 292,50 ± 70,34 mg de equivalente de catequina/g para o extrato AcOEt, que apresentou a melhor atividade antioxidante (IC50 50,40 ± 1,16 mg/mL) para o sequestro do DPPH. Podemos concluir que esta espécie apresenta forte atividade antibacteriana e antioxidante, bem como fraca atividade citotóxica.Brazilian Journal of BiologyAntioxidantesMoraceaeMorusAssessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccessSouza, G. R.Oliveira-Junior, Raimundo Gonçalves deDiniz, Tamara CoimbraBranco, ALexsandroLima-Saraiva, Sara Raquel G.Guimarães, A. L.Oliveira, Ana PaulaPacheco, A. G. M.Silva, M. G.Moraes Filho, Manoel Odorico deCosta, M. P.Pessoa, Cláudia Ó.Almeida, Jackson Roberto Guedes da SilvaORIGINAL2017_art_grsouza.pdf2017_art_grsouza.pdfapplication/pdf935848http://repositorio.ufc.br/bitstream/riufc/25426/1/2017_art_grsouza.pdf250b7982a2e3774c58b6872371e83a5dMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.ufc.br/bitstream/riufc/25426/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52riufc/254262021-12-02 16:40:12.614oai:repositorio.ufc.br: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Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2021-12-02T19:40:12Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
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