Evaluation of an alternative technique for preserving crustaceans in dry conditions with joint mobility: a proposal for didactic purposes

Although crustaceans are traditionally preserved in liquids (formaldehyde and/or ethyl alcohol), those substances tend to alter their morphological aspects. Glycerin, used in human anatomy, is considered a good substitute for formaldehyde, as it preserves animals in states similar to in vivo conditi...

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Detalles Bibliográficos
Autores: Costa, Amanda Brito, Silva, Márcio Borba da, Fraga, Ricardo Evangelista, Rocha, Agda Alves da, Nishiyama, Patrícia Belini, Anjos, Matheus Santos dos, Buchaim, Joaquim Jorge Silveira, Rocha, Mariane Amorim
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:Brasil
Institución:Universidade Estadual de Maringá (UEM)
Repositorio:Acta Scientiarum Biological Sciences
Idioma:inglés
OAI Identifier:oai:periodicos.uem.br/ojs:article/53450
Acceso en línea:http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/53450
Access Level:acceso abierto
Palabra clave:preservation; glycerin; Ucides cordatus; teaching.
Descripción
Sumario:Although crustaceans are traditionally preserved in liquids (formaldehyde and/or ethyl alcohol), those substances tend to alter their morphological aspects. Glycerin, used in human anatomy, is considered a good substitute for formaldehyde, as it preserves animals in states similar to in vivo conditions. There are no records in the literature, however, concerning the use of glycerin for conserving invertebrates. The objective of this work was to elaborate and evaluate alternative techniques for conserving the crustacean Ucides cordatus (Linnaeus, 1763). Six fixatives (1, 3, 4 and 5% formaldehyde, 70% alcohol, and dietrich solution) and two controls (positive and negative) were tested, as well as the effects of freezing before fixation on the integrity of U. cordatus specimens. Our results were evaluated with respect to nine variables. The treatments that demonstrated the best aesthetic results were 4% formaldehyde and 70% ethyl alcohol. The freezing of the animals resulted in brittle organs in all treatments tested. The technique discussed here is extremely promising for the conservation of animals for educational purposes, as it produces preserved specimens that are aesthetically similar to their in vivo conditions.