Glycerol, ethyleneglycol and cryopreservation influences on semen and sperm DNA-Protein complex in stallion
The cryopreservation process cause stress physical and chemical to the spermatozoa, causing biochemistry alteration, irreversible reduction of the spermatic motility, increase of the DNA degeneration and intrcellular enzyme and lipids release. The aim of this study was to evaluate the influence of n...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2006 |
| País: | Brasil |
| Institución: | Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
| Repositorio: | Brazilian Journal of Veterinary Research and Animal Science |
| Idioma: | portugués |
| OAI Identifier: | oai:revistas.usp.br:article/26537 |
| Acceso en línea: | https://www.revistas.usp.br/bjvras/article/view/26537 |
| Access Level: | acceso abierto |
| Palabra clave: | Garanhão Cromatina Sêmen Crioprotetores DNA Protamina Stallion Chromatin Semen Cryoprotectants Protamine |
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oai:revistas.usp.br:article/26537 |
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Brasil |
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| dc.title.none.fl_str_mv |
Glycerol, ethyleneglycol and cryopreservation influences on semen and sperm DNA-Protein complex in stallion Influência do glicerol e etilenoglicol e da criopreservação sobre o complexo DNA-Proteina de espermatozóides em garanhões |
| title |
Glycerol, ethyleneglycol and cryopreservation influences on semen and sperm DNA-Protein complex in stallion |
| spellingShingle |
Glycerol, ethyleneglycol and cryopreservation influences on semen and sperm DNA-Protein complex in stallion Brandão, Alessandra Cunha Garanhão Cromatina Sêmen Crioprotetores DNA Protamina Stallion Chromatin Semen Cryoprotectants DNA Protamine |
| title_short |
Glycerol, ethyleneglycol and cryopreservation influences on semen and sperm DNA-Protein complex in stallion |
| title_full |
Glycerol, ethyleneglycol and cryopreservation influences on semen and sperm DNA-Protein complex in stallion |
| title_fullStr |
Glycerol, ethyleneglycol and cryopreservation influences on semen and sperm DNA-Protein complex in stallion |
| title_full_unstemmed |
Glycerol, ethyleneglycol and cryopreservation influences on semen and sperm DNA-Protein complex in stallion |
| title_sort |
Glycerol, ethyleneglycol and cryopreservation influences on semen and sperm DNA-Protein complex in stallion |
| dc.creator.none.fl_str_mv |
Brandão, Alessandra Cunha Arruda, Rubens Paes de Madureira, Ed Hoffmann Martins, João Flávio Panattoni Assumpção, Mayra Elena Ortiz D'Ávila Visintin, José Antônio |
| author |
Brandão, Alessandra Cunha |
| author_facet |
Brandão, Alessandra Cunha Arruda, Rubens Paes de Madureira, Ed Hoffmann Martins, João Flávio Panattoni Assumpção, Mayra Elena Ortiz D'Ávila Visintin, José Antônio |
| author_role |
author |
| author2 |
Arruda, Rubens Paes de Madureira, Ed Hoffmann Martins, João Flávio Panattoni Assumpção, Mayra Elena Ortiz D'Ávila Visintin, José Antônio |
| author2_role |
author author author author author |
| dc.subject.por.fl_str_mv |
Garanhão Cromatina Sêmen Crioprotetores DNA Protamina Stallion Chromatin Semen Cryoprotectants DNA Protamine |
| topic |
Garanhão Cromatina Sêmen Crioprotetores DNA Protamina Stallion Chromatin Semen Cryoprotectants DNA Protamine |
| description |
The cryopreservation process cause stress physical and chemical to the spermatozoa, causing biochemistry alteration, irreversible reduction of the spermatic motility, increase of the DNA degeneration and intrcellular enzyme and lipids release. The aim of this study was to evaluate the influence of non-breeding and breeding seasons, glycerol and ethylene glycol, cryopreservation and thawing processes on stallion spermatozoa DNA-protein complex. It was compared fresh semen, diluted semen frozen without cryoprotectants, diluted semen exposured to cryoprotectants but not frozen and d) diluted semen frozen with cryoprotectants. Six stallions had 12 semen collections each. DNA-protein complex pathology was assessed by optical microscopy (1000x) using spermatozoa treated with ethanol-acetic acid 3:1 (v/v), HCl 4N at room temperature and toluidin blue 0,025% in McIlavaine buffer. Results showed that DNA-protein complex were different between frozen and not frozen spermatozoa groups (P<0,05). Frozen semen without cryoprotectants had no increasing of DNA-protein complex pathology compared to semen cryopreserved with cryoprotectant, but both showed increasing in relation to fresh and diluted semen exposured to cryoprotectants. The influence of non breeding and breeding season showed significant difference (P<0,05) in the fresh semen and fresh semen frozen without cryoprotectants. Cryopreservation process had negative influence on spermatozoa DNA-protein complex. |
| publishDate |
2006 |
| dc.date.none.fl_str_mv |
2006-12-03 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/26537 10.11606/issn.1678-4456.bjvras.2006.26537 |
| url |
https://www.revistas.usp.br/bjvras/article/view/26537 |
| identifier_str_mv |
10.11606/issn.1678-4456.bjvras.2006.26537 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
| dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/26537/28320 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
| publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
| dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 43 (2006): Suplemento; 68-73 Brazilian Journal of Veterinary Research and Animal Science; Vol. 43 (2006): Suplemento; 68-73 Brazilian Journal of Veterinary Research and Animal Science; v. 43 (2006): Suplemento; 68-73 Brazilian Journal of Veterinary Research and Animal Science; V. 43 (2006): Suplemento; 68-73 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
| instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
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USP |
| institution |
USP |
| reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
| collection |
Brazilian Journal of Veterinary Research and Animal Science |
| repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
| repository.mail.fl_str_mv |
bjvras@usp.br |
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1853662137623248896 |
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Glycerol, ethyleneglycol and cryopreservation influences on semen and sperm DNA-Protein complex in stallionInfluência do glicerol e etilenoglicol e da criopreservação sobre o complexo DNA-Proteina de espermatozóides em garanhõesGaranhãoCromatinaSêmenCrioprotetoresDNAProtaminaStallionChromatinSemenCryoprotectantsDNAProtamineThe cryopreservation process cause stress physical and chemical to the spermatozoa, causing biochemistry alteration, irreversible reduction of the spermatic motility, increase of the DNA degeneration and intrcellular enzyme and lipids release. The aim of this study was to evaluate the influence of non-breeding and breeding seasons, glycerol and ethylene glycol, cryopreservation and thawing processes on stallion spermatozoa DNA-protein complex. It was compared fresh semen, diluted semen frozen without cryoprotectants, diluted semen exposured to cryoprotectants but not frozen and d) diluted semen frozen with cryoprotectants. Six stallions had 12 semen collections each. DNA-protein complex pathology was assessed by optical microscopy (1000x) using spermatozoa treated with ethanol-acetic acid 3:1 (v/v), HCl 4N at room temperature and toluidin blue 0,025% in McIlavaine buffer. Results showed that DNA-protein complex were different between frozen and not frozen spermatozoa groups (P<0,05). Frozen semen without cryoprotectants had no increasing of DNA-protein complex pathology compared to semen cryopreserved with cryoprotectant, but both showed increasing in relation to fresh and diluted semen exposured to cryoprotectants. The influence of non breeding and breeding season showed significant difference (P<0,05) in the fresh semen and fresh semen frozen without cryoprotectants. Cryopreservation process had negative influence on spermatozoa DNA-protein complex.O processo de criopreservação causa estresse físico e químico aos espermatozóides, acarretando alterações bioquímicas, diminuição irreverssível da motilidade espermática, aumento da degeneração do DNA e liberação intracelular de enzimas e lipídeos. No presente estudo, foram estudadas a influência das estações não reprodutiva e reprodutiva, dos crioprotetores glicerol e etilenoglicol e do processo de congelação e descongelação sobre o complexo DNA-proteína de espermatozóides em garanhões. Foi comparados o sêmen puro, o sêmen puro e congelado sem crioprotetores, o sêmen diluído e exposto aos crioprotetores sem congelação e o sêmen diluído e congelado com crioprotetores. Foram utilizados seis garanhões, colhendo 12 ejaculados cada. A patologia do complexo DNA-Proteína foi avaliada em espermatozóides fixados com etanol-ácido-acético glacial 3:1 (v/v), tratados com HCL 4N a 25ºC e corados com azul de toluidina a 0,025% em tampão McIlvaine, empregando microscopia óptica com aumento de 1000x. Os resultados mostraram que a anomalia do complexo DNA-Proteína dos espermatozóides diferem entre os grupos congelados e não congelados (P<0,05). O sêmen congelado sem crioprotetor não apresentou aumento significativo de patologia do complexo DNA-Proteína em relação ao sêmen congelado com crioprotetores, mas ambos mostraram aumento em relação ao sêmen puro ou diluído e exposto aos crioprotetores. A influência da estação reprodutiva mostrou diferença significativa (P<0,05) somente no sêmen puro e no sêmen puro e congelado sem crioprotetor. Conclui-se que o processo de congelação exerce influência negativa sobre o complexo DNA-Proteína de espermatozóides em garanhões.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2006-12-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/2653710.11606/issn.1678-4456.bjvras.2006.26537Brazilian Journal of Veterinary Research and Animal Science; Vol. 43 (2006): Suplemento; 68-73Brazilian Journal of Veterinary Research and Animal Science; Vol. 43 (2006): Suplemento; 68-73Brazilian Journal of Veterinary Research and Animal Science; v. 43 (2006): Suplemento; 68-73Brazilian Journal of Veterinary Research and Animal Science; V. 43 (2006): Suplemento; 68-731678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/26537/28320info:eu-repo/semantics/openAccessBrandão, Alessandra CunhaArruda, Rubens Paes deMadureira, Ed HoffmannMartins, João Flávio PanattoniAssumpção, Mayra Elena Ortiz D'ÁvilaVisintin, José Antônio2020-06-23T04:16:03Zoai:revistas.usp.br:article/26537Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:48.891395Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
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15.198674 |