Avaliação de diferentes sistemas de cultivo in vitro para a micropropagação de Psychotria ipecacuanha (Brot.) Stokes

This work was carried out with Psychotria ipecacuanha, a Brazilian medicinal plant the roots of which contain emetine. The main objective was to develop a protocol for the micro-propagation of these species, by testing different culture techniques, the temporary immersion system, and the semi-solid...

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Authors: Batistini, A. P. [UNESP], Moro, J. R. [UNESP], França, S. C., Pereira, A. M S
Format: article
Status:Published version
Publication Date:2002
Country:Brasil
Institution:Universidade Estadual Paulista (UNESP)
Repository:Repositório Institucional da UNESP
Language:Portuguese
OAI Identifier:oai:repositorio.unesp.br:11449/66995
Online Access:http://www.ibb.unesp.br/Home/Departamentos/Botanica/RBPM-RevistaBrasileiradePlantasMedicinais/artigo5_v5_n1.pdf
http://hdl.handle.net/11449/66995
Access Level:Open access
Keyword:Alkaloids
Ipecac
Ipecacuanha
Medicinal plants
Rubiaceae
Tissue culture
emetine
Brazil
culture medium
culture technique
density
growth regulation
liquid culture
medicinal plant
micropropagation
plant growth
plant tissue culture
psychotria ipecacuanha
Cephaelis ipecacuanha
Psychotria ipecacuanha
id BR_9aceccf5ac39e3e4bcd2110c0d9f39b3
oai_identifier_str oai:repositorio.unesp.br:11449/66995
network_acronym_str BR
network_name_str Brasil
repository_id_str
spelling Avaliação de diferentes sistemas de cultivo in vitro para a micropropagação de Psychotria ipecacuanha (Brot.) StokesEvaluation of different systems of in vitro culture for propagation of Psychotria ipecacuanha (Brot.) StokesAlkaloidsIpecacIpecacuanhaMedicinal plantsRubiaceaeTissue cultureemetineBrazilculture mediumculture techniquedensitygrowth regulationliquid culturemedicinal plantmicropropagationplant growthplant tissue culturepsychotria ipecacuanhaCephaelis ipecacuanhaPsychotria ipecacuanhaThis work was carried out with Psychotria ipecacuanha, a Brazilian medicinal plant the roots of which contain emetine. The main objective was to develop a protocol for the micro-propagation of these species, by testing different culture techniques, the temporary immersion system, and the semi-solid and liquid media systems. In the semi-solid system, experiments were developed in flasks of two different sizes containing MS, B5, and WP media to which were added different growth regulators. Innoculum density was also evaluated. The liquid medium system consisted of MS medium supplemented with different growth regulators. For the temporary immersion system, the MS medium received an addition of 1.5mg/L BAP and 0.5mg/L GA3, and a reverse digital apparatus and vacuum pump were used. The liquid medium system with MS medium supplemented with 1.5mg/L BAP and 0.5mg/L GA3 presented the best results for shoot proliferation in a period of 30 days in culture (2.37 ± 0.32 shoots/explant). Cultures carried out for 90 days in the semi-solid system, using 8.5 × 5.5cm flasks and 3 explants per flask, developed 1.80 ± 0.20 shoots/explant, achieving 3.06 ± 0.51 cm of height adn presented superior survival ratio (96%). Explants cultured in temporary immersion system for 90 days showed 2.30 ± 1.10 shoots/explant achieving a growth of 2.08 ± 0.12 cm and 52% survival.UNESP/Jaboticabal, São Paulo, CEP 14870-000UNAERP/Ribeirao Preto, São Paulo, CEP 14096-380UNESP/Jaboticabal, São Paulo, CEP 14870-000Universidade Estadual Paulista (Unesp)UNAERP/Ribeirao Preto2014-05-27T11:20:31Z2014-05-27T11:20:31Z2002-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article27-35application/pdfhttp://www.ibb.unesp.br/Home/Departamentos/Botanica/RBPM-RevistaBrasileiradePlantasMedicinais/artigo5_v5_n1.pdfRevista Brasileira de Plantas Medicinais, v. 5, n. 1, p. 27-35, 2002.1516-0572http://hdl.handle.net/11449/669952-s2.0-00368193442-s2.0-0036819344.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporRevista Brasileira de Plantas Medicinais0,199info:eu-repo/semantics/openAccessBatistini, A. P. [UNESP]Moro, J. R. [UNESP]França, S. C.Pereira, A. M S2025-10-22T05:12:53Zoai:repositorio.unesp.br:11449/66995Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-10-22T05:12:53Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Avaliação de diferentes sistemas de cultivo in vitro para a micropropagação de Psychotria ipecacuanha (Brot.) Stokes
Evaluation of different systems of in vitro culture for propagation of Psychotria ipecacuanha (Brot.) Stokes
title Avaliação de diferentes sistemas de cultivo in vitro para a micropropagação de Psychotria ipecacuanha (Brot.) Stokes
spellingShingle Avaliação de diferentes sistemas de cultivo in vitro para a micropropagação de Psychotria ipecacuanha (Brot.) Stokes
Batistini, A. P. [UNESP]
Alkaloids
Ipecac
Ipecacuanha
Medicinal plants
Rubiaceae
Tissue culture
emetine
Brazil
culture medium
culture technique
density
growth regulation
liquid culture
medicinal plant
micropropagation
plant growth
plant tissue culture
psychotria ipecacuanha
Cephaelis ipecacuanha
Psychotria ipecacuanha
title_short Avaliação de diferentes sistemas de cultivo in vitro para a micropropagação de Psychotria ipecacuanha (Brot.) Stokes
title_full Avaliação de diferentes sistemas de cultivo in vitro para a micropropagação de Psychotria ipecacuanha (Brot.) Stokes
title_fullStr Avaliação de diferentes sistemas de cultivo in vitro para a micropropagação de Psychotria ipecacuanha (Brot.) Stokes
title_full_unstemmed Avaliação de diferentes sistemas de cultivo in vitro para a micropropagação de Psychotria ipecacuanha (Brot.) Stokes
title_sort Avaliação de diferentes sistemas de cultivo in vitro para a micropropagação de Psychotria ipecacuanha (Brot.) Stokes
dc.creator.none.fl_str_mv Batistini, A. P. [UNESP]
Moro, J. R. [UNESP]
França, S. C.
Pereira, A. M S
author Batistini, A. P. [UNESP]
author_facet Batistini, A. P. [UNESP]
Moro, J. R. [UNESP]
França, S. C.
Pereira, A. M S
author_role author
author2 Moro, J. R. [UNESP]
França, S. C.
Pereira, A. M S
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
UNAERP/Ribeirao Preto
dc.subject.por.fl_str_mv Alkaloids
Ipecac
Ipecacuanha
Medicinal plants
Rubiaceae
Tissue culture
emetine
Brazil
culture medium
culture technique
density
growth regulation
liquid culture
medicinal plant
micropropagation
plant growth
plant tissue culture
psychotria ipecacuanha
Cephaelis ipecacuanha
Psychotria ipecacuanha
topic Alkaloids
Ipecac
Ipecacuanha
Medicinal plants
Rubiaceae
Tissue culture
emetine
Brazil
culture medium
culture technique
density
growth regulation
liquid culture
medicinal plant
micropropagation
plant growth
plant tissue culture
psychotria ipecacuanha
Cephaelis ipecacuanha
Psychotria ipecacuanha
description This work was carried out with Psychotria ipecacuanha, a Brazilian medicinal plant the roots of which contain emetine. The main objective was to develop a protocol for the micro-propagation of these species, by testing different culture techniques, the temporary immersion system, and the semi-solid and liquid media systems. In the semi-solid system, experiments were developed in flasks of two different sizes containing MS, B5, and WP media to which were added different growth regulators. Innoculum density was also evaluated. The liquid medium system consisted of MS medium supplemented with different growth regulators. For the temporary immersion system, the MS medium received an addition of 1.5mg/L BAP and 0.5mg/L GA3, and a reverse digital apparatus and vacuum pump were used. The liquid medium system with MS medium supplemented with 1.5mg/L BAP and 0.5mg/L GA3 presented the best results for shoot proliferation in a period of 30 days in culture (2.37 ± 0.32 shoots/explant). Cultures carried out for 90 days in the semi-solid system, using 8.5 × 5.5cm flasks and 3 explants per flask, developed 1.80 ± 0.20 shoots/explant, achieving 3.06 ± 0.51 cm of height adn presented superior survival ratio (96%). Explants cultured in temporary immersion system for 90 days showed 2.30 ± 1.10 shoots/explant achieving a growth of 2.08 ± 0.12 cm and 52% survival.
publishDate 2002
dc.date.none.fl_str_mv 2002-10-01
2014-05-27T11:20:31Z
2014-05-27T11:20:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.ibb.unesp.br/Home/Departamentos/Botanica/RBPM-RevistaBrasileiradePlantasMedicinais/artigo5_v5_n1.pdf
Revista Brasileira de Plantas Medicinais, v. 5, n. 1, p. 27-35, 2002.
1516-0572
http://hdl.handle.net/11449/66995
2-s2.0-0036819344
2-s2.0-0036819344.pdf
url http://www.ibb.unesp.br/Home/Departamentos/Botanica/RBPM-RevistaBrasileiradePlantasMedicinais/artigo5_v5_n1.pdf
http://hdl.handle.net/11449/66995
identifier_str_mv Revista Brasileira de Plantas Medicinais, v. 5, n. 1, p. 27-35, 2002.
1516-0572
2-s2.0-0036819344
2-s2.0-0036819344.pdf
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Revista Brasileira de Plantas Medicinais
0,199
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 27-35
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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score 15,198674

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