Caracterização química dos géis produzidos pelas bactérias diazotróficas Rhizobium tropici e Mesorhizobium sp

The exopolysaccharides with characteristics of gel produced by Rhizobium tropici (EPS RT) and Mesorhizobium sp (EPS MR) are acidic heteropolysaccharide composed mainly of glucose and galactose in a molar ratio of 4:1 and 5:1 respectively, with traces of mannose (∼1%). Chemical analysis showed the pr...

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Detalhes bibliográficos
Autores: Monteiro, Nilson Kobori [UNESP], Aranda-Selverio, Gabriel [UNESP], Exposti, Diego Tadeu Degli [UNESP], De Lourdes Corradi Da Silva, Maria [UNESP], Lemos, Eliana Gertrudes Macedo [UNESP], Campanharo, João Carlos [UNESP], Silveira, Joana Léa Meira
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2012
País:Brasil
Recursos:Universidade Estadual Paulista (UNESP)
Repositório:Repositório Institucional da UNESP
Idioma:português
OAI Identifier:oai:repositorio.unesp.br:11449/226865
Acesso em linha:http://dx.doi.org/10.1590/S0100-40422012000400009
http://hdl.handle.net/11449/226865
Access Level:Acceso aberto
Palavra-chave:Acid exopolysaccharide
Mesorhizobium sp
Rhizobium tropici
Descrição
Resumo:The exopolysaccharides with characteristics of gel produced by Rhizobium tropici (EPS RT) and Mesorhizobium sp (EPS MR) are acidic heteropolysaccharide composed mainly of glucose and galactose in a molar ratio of 4:1 and 5:1 respectively, with traces of mannose (∼1%). Chemical analysis showed the presence of uronic acid, pyruvate and acetyl-substituents in the structures of both polymers. Experiments of gel permeation chromatography and polyacrylamide gel electrophoresis showed that EPS RT and EPS MR are homogeneous molecules with low grade of polydispersity. The EPS were characterized using spectroscopic techniques of FT-IR, 1H and 13C-NMR.

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