Isolation of rickettsia rickettsii from the tick amblyomma sculptum from a brazilian spotted fever-endemic area in the pampulha lake region, southeastern brazil

Brazilian spotted fever (BSF) is a tick-borne disease caused by the bacterium Rickettsia rickettsii, the deadliest spotted fever of the world, transmitted in southeastern Brazil mainly by the tick Amblyomma sculptum, a member of the Amblyomma cajennense species complex. In the present study, over 50...

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Autores: Marcelo B.labruna, Romario c. Leite, Felipe s. Krawczak, Monize Gerardi, Lina c. Binder, Amalia R.m. Barbieri, Gustavo f. Paz, Daniel s. Rodrigues, Ricardo Nascimento Araujo, Marcela Lanza Bernardes
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Brasil
Institución:Universidade Federal de Minas Gerais (UFMG)
Repositorio:Repositório Institucional da UFMG
Idioma:inglés
OAI Identifier:oai:repositorio.ufmg.br:1843/39793
Acceso en línea:http://hdl.handle.net/1843/39793
Access Level:acceso abierto
Palabra clave:Parasitology
Public health
Rickettsioses
tick
Parasitologia
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spelling Isolation of rickettsia rickettsii from the tick amblyomma sculptum from a brazilian spotted fever-endemic area in the pampulha lake region, southeastern brazilIsolation of rickettsia rickettsii from the tick amblyomma sculptum from a brazilian spotted fever-endemic area in the pampulha lake region, southeastern brazilParasitologyPublic healthRickettsiosestickParasitologiaBrazilian spotted fever (BSF) is a tick-borne disease caused by the bacterium Rickettsia rickettsii, the deadliest spotted fever of the world, transmitted in southeastern Brazil mainly by the tick Amblyomma sculptum, a member of the Amblyomma cajennense species complex. In the present study, over 5000 adults of A. sculptum ticks were collected by dry ice traps in the Municipal Ecological Park, alongside the Pampulha Lake region, a BSF-endemic area of Belo Horizonte city, state of Minas Gerais, southeastern Brazil. Ticks were taken alive to the laboratory, where a sample of 2100 specimens was processed for isolation of R. rickettsii. For this purpose, ticks were macerated and intraperitoneally inoculated into guinea pigs. Only one out of 21 inoculated guinea pigs presented high fever within 21days post inoculation with tick homogenates. This febrile animal was euthanized and its internal organs were macerated and inoculated into additional guinea pigs (guinea pig passage). A spleen sample from a febrile guinea pig was used to inoculate Vero cells, resulting in a successful isolation and in vitro establishment of rickettsiae. Rickettsia-infected Vero cells were used for molecular characterization of the rickettsial isolate through PCR and DNA sequencing of fragments of three rickettsial genes (gltA, ompA, and ompB), which were all 100% identical to corresponding sequences of R. rickettsii from GenBank. The present R. rickettsii isolate was designated as strain Pampulha. A minimal infection rate of 0.05% R. rickettsii-infected ticks was estimated for A. sculptum population of the Pampulha Lake region. Our results, coupled with epidemiological evidences, suggest that R. rickettsii strain Pampulha, isolated from A. sculptum ticks in the present study, is the strain responsible for human clinical cases of BSF in the Pampulha Lake region of Belo Horizonte city.Universidade Federal de Minas GeraisBrasilICB - DEPARTAMENTO DE PARASITOLOGIAVET - DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVAUFMG2022-03-04T13:45:03Z2022-03-04T13:45:03Z2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf10.1016/j.vprsr.2017.02.00724059390http://hdl.handle.net/1843/39793engVeterinary parasitology: regional studies and reportsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGMarcelo B.labrunaRomario c. LeiteFelipe s. KrawczakMonize GerardiLina c. BinderAmalia R.m. BarbieriGustavo f. PazDaniel s. RodriguesRicardo Nascimento AraujoMarcela Lanza Bernardes2022-03-04T13:45:04Zoai:repositorio.ufmg.br:1843/39793Repositório InstitucionalPUBhttps://repositorio.ufmg.br/oairepositorio@ufmg.bropendoar:2022-03-04T13:45:04Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Isolation of rickettsia rickettsii from the tick amblyomma sculptum from a brazilian spotted fever-endemic area in the pampulha lake region, southeastern brazil
Isolation of rickettsia rickettsii from the tick amblyomma sculptum from a brazilian spotted fever-endemic area in the pampulha lake region, southeastern brazil
title Isolation of rickettsia rickettsii from the tick amblyomma sculptum from a brazilian spotted fever-endemic area in the pampulha lake region, southeastern brazil
spellingShingle Isolation of rickettsia rickettsii from the tick amblyomma sculptum from a brazilian spotted fever-endemic area in the pampulha lake region, southeastern brazil
Marcelo B.labruna
Parasitology
Public health
Rickettsioses
tick
Parasitologia
title_short Isolation of rickettsia rickettsii from the tick amblyomma sculptum from a brazilian spotted fever-endemic area in the pampulha lake region, southeastern brazil
title_full Isolation of rickettsia rickettsii from the tick amblyomma sculptum from a brazilian spotted fever-endemic area in the pampulha lake region, southeastern brazil
title_fullStr Isolation of rickettsia rickettsii from the tick amblyomma sculptum from a brazilian spotted fever-endemic area in the pampulha lake region, southeastern brazil
title_full_unstemmed Isolation of rickettsia rickettsii from the tick amblyomma sculptum from a brazilian spotted fever-endemic area in the pampulha lake region, southeastern brazil
title_sort Isolation of rickettsia rickettsii from the tick amblyomma sculptum from a brazilian spotted fever-endemic area in the pampulha lake region, southeastern brazil
dc.creator.none.fl_str_mv Marcelo B.labruna
Romario c. Leite
Felipe s. Krawczak
Monize Gerardi
Lina c. Binder
Amalia R.m. Barbieri
Gustavo f. Paz
Daniel s. Rodrigues
Ricardo Nascimento Araujo
Marcela Lanza Bernardes
author Marcelo B.labruna
author_facet Marcelo B.labruna
Romario c. Leite
Felipe s. Krawczak
Monize Gerardi
Lina c. Binder
Amalia R.m. Barbieri
Gustavo f. Paz
Daniel s. Rodrigues
Ricardo Nascimento Araujo
Marcela Lanza Bernardes
author_role author
author2 Romario c. Leite
Felipe s. Krawczak
Monize Gerardi
Lina c. Binder
Amalia R.m. Barbieri
Gustavo f. Paz
Daniel s. Rodrigues
Ricardo Nascimento Araujo
Marcela Lanza Bernardes
author2_role author
author
author
author
author
author
author
author
author
dc.subject.por.fl_str_mv Parasitology
Public health
Rickettsioses
tick
Parasitologia
topic Parasitology
Public health
Rickettsioses
tick
Parasitologia
description Brazilian spotted fever (BSF) is a tick-borne disease caused by the bacterium Rickettsia rickettsii, the deadliest spotted fever of the world, transmitted in southeastern Brazil mainly by the tick Amblyomma sculptum, a member of the Amblyomma cajennense species complex. In the present study, over 5000 adults of A. sculptum ticks were collected by dry ice traps in the Municipal Ecological Park, alongside the Pampulha Lake region, a BSF-endemic area of Belo Horizonte city, state of Minas Gerais, southeastern Brazil. Ticks were taken alive to the laboratory, where a sample of 2100 specimens was processed for isolation of R. rickettsii. For this purpose, ticks were macerated and intraperitoneally inoculated into guinea pigs. Only one out of 21 inoculated guinea pigs presented high fever within 21days post inoculation with tick homogenates. This febrile animal was euthanized and its internal organs were macerated and inoculated into additional guinea pigs (guinea pig passage). A spleen sample from a febrile guinea pig was used to inoculate Vero cells, resulting in a successful isolation and in vitro establishment of rickettsiae. Rickettsia-infected Vero cells were used for molecular characterization of the rickettsial isolate through PCR and DNA sequencing of fragments of three rickettsial genes (gltA, ompA, and ompB), which were all 100% identical to corresponding sequences of R. rickettsii from GenBank. The present R. rickettsii isolate was designated as strain Pampulha. A minimal infection rate of 0.05% R. rickettsii-infected ticks was estimated for A. sculptum population of the Pampulha Lake region. Our results, coupled with epidemiological evidences, suggest that R. rickettsii strain Pampulha, isolated from A. sculptum ticks in the present study, is the strain responsible for human clinical cases of BSF in the Pampulha Lake region of Belo Horizonte city.
publishDate 2017
dc.date.none.fl_str_mv 2017
2022-03-04T13:45:03Z
2022-03-04T13:45:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 10.1016/j.vprsr.2017.02.007
24059390
http://hdl.handle.net/1843/39793
identifier_str_mv 10.1016/j.vprsr.2017.02.007
24059390
url http://hdl.handle.net/1843/39793
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Veterinary parasitology: regional studies and reports
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
Brasil
ICB - DEPARTAMENTO DE PARASITOLOGIA
VET - DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVA
UFMG
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
Brasil
ICB - DEPARTAMENTO DE PARASITOLOGIA
VET - DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVA
UFMG
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
repository.name.fl_str_mv Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv repositorio@ufmg.br
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score 15,301629

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