Oxidative stress in the latissimus dorsi muscle of diabetic rats

The purpose of the present study was to investigate the effects of experimental diabetes on the oxidant and antioxidant status of latissimus dorsi (LD) muscles of male Wistar rats (220 ± 5 g, N = 11). Shortterm (5 days) diabetes was induced by a single injection of streptozotocin (STZ, 50 mg/kg, iv;...

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Detalles Bibliográficos
Autores: D'Avila, Katia de Angelis Lobo, Cestari, Idagene Aparecida, Barp, Jaqueline, Dall'Ago, Pedro, Piedras, Tania Regina Gattelli Fernandes, Bittencourt Junior, Paulo Ivo Homem de, Belló-Klein, Adriane, Bello, Antonio Andrea, Llesuy, Susana Francisca, Irigoyen, Maria Claudia Costa
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2000
País:Brasil
Institución:Universidade Federal do Rio Grande do Sul (UFRGS)
Repositorio:Repositório Institucional da UFRGS
Idioma:inglés
OAI Identifier:oai:www.lume.ufrgs.br:10183/21175
Acceso en línea:http://hdl.handle.net/10183/21175
Access Level:acceso abierto
Palabra clave:Diabetes mellitus
Fisiologia
Streptozotocin-diabetes
Latissimus dorsi muscle
Oxidative stress
Catalase
Glutathione
Descripción
Sumario:The purpose of the present study was to investigate the effects of experimental diabetes on the oxidant and antioxidant status of latissimus dorsi (LD) muscles of male Wistar rats (220 ± 5 g, N = 11). Shortterm (5 days) diabetes was induced by a single injection of streptozotocin (STZ, 50 mg/kg, iv; glycemia >300 mg/dl). LD muscle of STZdiabetic rats presented higher levels of thiobarbituric acid reactive substances (TBARS) and chemiluminescence (0.36 ± 0.02 nmol/mg protein and 14706 ± 1581 cps/mg protein) than LD muscle of normal rats (0.23 ± 0.04 nmol/mg protein and 7389 ± 1355 cps/mg protein). Diabetes induced a 92% increase in catalase and a 27% increase in glutathione S-transferase activities in LD muscle. Glutathione peroxidase activity was reduced (58%) in STZ-diabetic rats and superoxide dismutase activity was similar in LD muscle of both groups. A positive correlation was obtained between catalase activity and the oxidative stress of LD, as evaluated in terms of TBARS (r = 0.78) and by chemiluminescence (r = 0.89). Catalase activity also correlated inversely with glutathione peroxidase activity (r = 0.79). These data suggest that an increased oxidative stress in LD muscle of diabetic rats may be related to skeletal muscle myopathy.

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