ESTs from a wild Arachis species for gene discovery and marker development.

Background Due to its origin, peanut has a very narrow genetic background. Wild relatives can be a source of genetic variability for cultivated peanut. In this study, the transcriptome of the wild species Arachis stenosperma accession V10309 was analyzed. Results ESTs were produced from four cDNA li...

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Autores: PROITE, K., LEAL-BERTIOLI, S. C. M., BERTIOLI, D. J., MORETZSOHN, M. C., SILVA, F. R. da, MARTINS, N. F., GUIMARÃES, P. M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2007
País:Brasil
Institución:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
Repositorio:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Idioma:inglés
OAI Identifier:oai:www.alice.cnptia.embrapa.br:doc/187868
Acceso en línea:http://www.alice.cnptia.embrapa.br/alice/handle/doc/187868
Access Level:acceso abierto
Palabra clave:Arachis stenosperma
Amendoim silvestre
ESTs
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spelling ESTs from a wild Arachis species for gene discovery and marker development.Arachis stenospermaAmendoim silvestreESTsBackground Due to its origin, peanut has a very narrow genetic background. Wild relatives can be a source of genetic variability for cultivated peanut. In this study, the transcriptome of the wild species Arachis stenosperma accession V10309 was analyzed. Results ESTs were produced from four cDNA libraries of RNAs extracted from leaves and roots of A. stenosperma. Randomly selected cDNA clones were sequenced to generate 8,785 ESTs, of which 6,264 (71.3%) had high quality, with 3,500 clusters: 963 contigs and 2537 singlets. Only 55.9% matched homologous sequences of known genes. ESTs were classified into 23 different categories according to putative protein functions. Numerous sequences related to disease resistance, drought tolerance and human health were identified. Two hundred and six microsatellites were found and markers have been developed for 188 of these. The microsatellite profile was analyzed and compared to other transcribed and genomic sequence data. Conclusion This is, to date, the first report on the analysis of transcriptome of a wild relative of peanut. The ESTs produced in this study are a valuable resource for gene discovery, the characterization of new wild alleles, and for marker development. The ESTs were released in the [GenBank:EH041934 to EH048197].KARINA PROITESORAYA CRISTINA DE MACEDO LEAL BERTIOLI, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIADAVID J. BERTIOLI, UNIVERSIDADE CATÓLICA DE BRASÍLIAMARCIO DE CARVALHO MORETZSOHN, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIAFELIPE RODRIGUES DA SILVA, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIANATALIA FLORÊNCIO MARTINS, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIAPATRÍCIA MESSEMBERG GUIMARÃES, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA.2018-06-06T00:57:13Z2018-06-06T00:57:13Z2007-05-1120072018-06-06T00:57:13Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBMC Plant Biology, v. 7, n. 7, online, 2007.http://www.alice.cnptia.embrapa.br/alice/handle/doc/187868enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAPROITE, K.LEAL-BERTIOLI, S. C. M.BERTIOLI, D. J.MORETZSOHN, M. C.SILVA, F. R. daMARTINS, N. F.GUIMARÃES, P. M.2018-06-06T00:57:20Zoai:www.alice.cnptia.embrapa.br:doc/187868Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-06-06T00:57:20Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv ESTs from a wild Arachis species for gene discovery and marker development.
title ESTs from a wild Arachis species for gene discovery and marker development.
spellingShingle ESTs from a wild Arachis species for gene discovery and marker development.
PROITE, K.
Arachis stenosperma
Amendoim silvestre
ESTs
title_short ESTs from a wild Arachis species for gene discovery and marker development.
title_full ESTs from a wild Arachis species for gene discovery and marker development.
title_fullStr ESTs from a wild Arachis species for gene discovery and marker development.
title_full_unstemmed ESTs from a wild Arachis species for gene discovery and marker development.
title_sort ESTs from a wild Arachis species for gene discovery and marker development.
dc.creator.none.fl_str_mv PROITE, K.
LEAL-BERTIOLI, S. C. M.
BERTIOLI, D. J.
MORETZSOHN, M. C.
SILVA, F. R. da
MARTINS, N. F.
GUIMARÃES, P. M.
author PROITE, K.
author_facet PROITE, K.
LEAL-BERTIOLI, S. C. M.
BERTIOLI, D. J.
MORETZSOHN, M. C.
SILVA, F. R. da
MARTINS, N. F.
GUIMARÃES, P. M.
author_role author
author2 LEAL-BERTIOLI, S. C. M.
BERTIOLI, D. J.
MORETZSOHN, M. C.
SILVA, F. R. da
MARTINS, N. F.
GUIMARÃES, P. M.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv KARINA PROITE
SORAYA CRISTINA DE MACEDO LEAL BERTIOLI, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA
DAVID J. BERTIOLI, UNIVERSIDADE CATÓLICA DE BRASÍLIA
MARCIO DE CARVALHO MORETZSOHN, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA
FELIPE RODRIGUES DA SILVA, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA
NATALIA FLORÊNCIO MARTINS, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA
PATRÍCIA MESSEMBERG GUIMARÃES, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA.
dc.subject.por.fl_str_mv Arachis stenosperma
Amendoim silvestre
ESTs
topic Arachis stenosperma
Amendoim silvestre
ESTs
description Background Due to its origin, peanut has a very narrow genetic background. Wild relatives can be a source of genetic variability for cultivated peanut. In this study, the transcriptome of the wild species Arachis stenosperma accession V10309 was analyzed. Results ESTs were produced from four cDNA libraries of RNAs extracted from leaves and roots of A. stenosperma. Randomly selected cDNA clones were sequenced to generate 8,785 ESTs, of which 6,264 (71.3%) had high quality, with 3,500 clusters: 963 contigs and 2537 singlets. Only 55.9% matched homologous sequences of known genes. ESTs were classified into 23 different categories according to putative protein functions. Numerous sequences related to disease resistance, drought tolerance and human health were identified. Two hundred and six microsatellites were found and markers have been developed for 188 of these. The microsatellite profile was analyzed and compared to other transcribed and genomic sequence data. Conclusion This is, to date, the first report on the analysis of transcriptome of a wild relative of peanut. The ESTs produced in this study are a valuable resource for gene discovery, the characterization of new wild alleles, and for marker development. The ESTs were released in the [GenBank:EH041934 to EH048197].
publishDate 2007
dc.date.none.fl_str_mv 2007-05-11
2007
2018-06-06T00:57:13Z
2018-06-06T00:57:13Z
2018-06-06T00:57:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv BMC Plant Biology, v. 7, n. 7, online, 2007.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/187868
identifier_str_mv BMC Plant Biology, v. 7, n. 7, online, 2007.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/187868
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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