Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system
In recent years, proteasome involvement in the damage response induced by ionizing radiation (IR) became evident. However, whether proteasome plays a direct or indirect role in IR-induced damage response still unclear. Trypanosoma cruzi is a human parasite capable of remarkable high tolerance to IR,...
| Autores: | , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2017 |
| País: | Brasil |
| Recursos: | Universidade Federal de São Paulo (UNIFESP) |
| Repositorio: | Repositório Institucional da UNIFESP |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unifesp.br:11600/54992 |
| Acesso em linha: | https://dx.doi.org/10.1016/j.molbiopara.2017.01.005 https://repositorio.unifesp.br/handle/11600/54992 |
| Access Level: | acceso abierto |
| Palavra-chave: | Trypanosoma cruzi Proteasome Ionizing radiation |
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oai:repositorio.unifesp.br:11600/54992 |
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| dc.title.none.fl_str_mv |
Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system |
| title |
Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system |
| spellingShingle |
Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system Cerqueira, Paula Gonçalves Trypanosoma cruzi Proteasome Ionizing radiation |
| title_short |
Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system |
| title_full |
Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system |
| title_fullStr |
Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system |
| title_full_unstemmed |
Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system |
| title_sort |
Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system |
| dc.creator.none.fl_str_mv |
Cerqueira, Paula Gonçalves Passos-Silva, Danielle Gomes Vieira-da-Rocha, João Pedro Mendes, Isabela Cecilia Oliveira, Karla Andrade de Oliveira, Camila Franco Batista de Vilela, Liza Figueiredo Felicori Nagem, Ronaldo Alves Pinto Cardoso, Joseane Nardelli, Sheila Cristina Krieger, Marco Aurélio Franco, Gloria Regina Macedo, Andrea Mara Pena, Sergio Danilo Junho Schenkman, Sergio [UNIFESP] Gomes, Dawidson A. Guerra-Sa, Renata Machado, Carlos Renato |
| author |
Cerqueira, Paula Gonçalves |
| author_facet |
Cerqueira, Paula Gonçalves Passos-Silva, Danielle Gomes Vieira-da-Rocha, João Pedro Mendes, Isabela Cecilia Oliveira, Karla Andrade de Oliveira, Camila Franco Batista de Vilela, Liza Figueiredo Felicori Nagem, Ronaldo Alves Pinto Cardoso, Joseane Nardelli, Sheila Cristina Krieger, Marco Aurélio Franco, Gloria Regina Macedo, Andrea Mara Pena, Sergio Danilo Junho Schenkman, Sergio [UNIFESP] Gomes, Dawidson A. Guerra-Sa, Renata Machado, Carlos Renato |
| author_role |
author |
| author2 |
Passos-Silva, Danielle Gomes Vieira-da-Rocha, João Pedro Mendes, Isabela Cecilia Oliveira, Karla Andrade de Oliveira, Camila Franco Batista de Vilela, Liza Figueiredo Felicori Nagem, Ronaldo Alves Pinto Cardoso, Joseane Nardelli, Sheila Cristina Krieger, Marco Aurélio Franco, Gloria Regina Macedo, Andrea Mara Pena, Sergio Danilo Junho Schenkman, Sergio [UNIFESP] Gomes, Dawidson A. Guerra-Sa, Renata Machado, Carlos Renato |
| author2_role |
author author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
| dc.subject.por.fl_str_mv |
Trypanosoma cruzi Proteasome Ionizing radiation |
| topic |
Trypanosoma cruzi Proteasome Ionizing radiation |
| description |
In recent years, proteasome involvement in the damage response induced by ionizing radiation (IR) became evident. However, whether proteasome plays a direct or indirect role in IR-induced damage response still unclear. Trypanosoma cruzi is a human parasite capable of remarkable high tolerance to IR, suggesting a highly efficient damage response system. Here, we investigate the role of T. cruzi proteasome in the damage response induced by IR. We exposed epimastigotes to high doses of gamma ray and we analyzed the expression and subcellular localization of several components of the ubiquitin-proteasome system. We show that proteasome inhibition increases IR-induced cell growth arrest and proteasome-mediated proteolysis is altered after parasite exposure. We observed nuclear accumulation of 19S and 20S proteasome subunits in response to IR treatments. Intriguingly, the dynamic of 19S particle nuclear accumulation was more similar to the dynamic observed for Rad51 nuclear translocation than the observed for 20S. In the other hand, 20S increase and nuclear translocation could be related with an increase of its regulator PA26 and high levels of proteasome-mediated proteolysis in vitro. The intersection between the opposed peaks of 19S and 20S protein levels was marked by nuclear accumulation of both 20S and 19S together with Ubiquitin, suggesting a role of ubiquitin-proteasome system in the nuclear protein turnover at the time. Our results revealed the importance of proteasome-mediated proteolysis in T. cruzi IR-induced damage response suggesting that proteasome is also involved in T. cruzi IR tolerance. Moreover, our data support the possible direct/signaling role of 19S in DNA damage repair. Based on these results, we speculate that spatial and temporal differences between the 19S particle and 20S proteasome controls proteasome multiple roles in IR damage response. (C) 2017 Elsevier B.V. All rights reserved. |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017 2020-07-17T14:02:45Z 2020-07-17T14:02:45Z |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://dx.doi.org/10.1016/j.molbiopara.2017.01.005 Molecular And Biochemical Parasitology. Amsterdam, v. 212, p. 55-67, 2017. 10.1016/j.molbiopara.2017.01.005 0166-6851 https://repositorio.unifesp.br/handle/11600/54992 WOS:000397374700008 |
| dc.identifier.dark.fl_str_mv |
ark:/48912/001300001xxp3 |
| url |
https://dx.doi.org/10.1016/j.molbiopara.2017.01.005 https://repositorio.unifesp.br/handle/11600/54992 |
| identifier_str_mv |
Molecular And Biochemical Parasitology. Amsterdam, v. 212, p. 55-67, 2017. 10.1016/j.molbiopara.2017.01.005 0166-6851 WOS:000397374700008 ark:/48912/001300001xxp3 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Molecular And Biochemical Parasitology |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
55-67 |
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Amsterdam |
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Elsevier Science Bv |
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Elsevier Science Bv |
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reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
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UNIFESP |
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UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
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biblioteca.csp@unifesp.br |
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1853670510415577088 |
| spelling |
Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome systemTrypanosoma cruziProteasomeIonizing radiationIn recent years, proteasome involvement in the damage response induced by ionizing radiation (IR) became evident. However, whether proteasome plays a direct or indirect role in IR-induced damage response still unclear. Trypanosoma cruzi is a human parasite capable of remarkable high tolerance to IR, suggesting a highly efficient damage response system. Here, we investigate the role of T. cruzi proteasome in the damage response induced by IR. We exposed epimastigotes to high doses of gamma ray and we analyzed the expression and subcellular localization of several components of the ubiquitin-proteasome system. We show that proteasome inhibition increases IR-induced cell growth arrest and proteasome-mediated proteolysis is altered after parasite exposure. We observed nuclear accumulation of 19S and 20S proteasome subunits in response to IR treatments. Intriguingly, the dynamic of 19S particle nuclear accumulation was more similar to the dynamic observed for Rad51 nuclear translocation than the observed for 20S. In the other hand, 20S increase and nuclear translocation could be related with an increase of its regulator PA26 and high levels of proteasome-mediated proteolysis in vitro. The intersection between the opposed peaks of 19S and 20S protein levels was marked by nuclear accumulation of both 20S and 19S together with Ubiquitin, suggesting a role of ubiquitin-proteasome system in the nuclear protein turnover at the time. Our results revealed the importance of proteasome-mediated proteolysis in T. cruzi IR-induced damage response suggesting that proteasome is also involved in T. cruzi IR tolerance. Moreover, our data support the possible direct/signaling role of 19S in DNA damage repair. Based on these results, we speculate that spatial and temporal differences between the 19S particle and 20S proteasome controls proteasome multiple roles in IR damage response. (C) 2017 Elsevier B.V. All rights reserved.Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim Imunol, Belo Horizonte, MG, BrazilUniv Fed Sao Paulo, Dept Microbiol Imunol Parasitol, Sao Paulo, BrazilUniv Fed Ouro Preto, Inst Ciencias Exatas Biol, Dept Ciencias Biol & Nucleo Pesquisa Ciencias Bio, Ouro Preto, BrazilInst Carlos Chagas, FIOCRUZ, Curitiba, Parana, BrazilInst Biol Mol Parana, Curitiba, Parana, BrazilUniv Fed Sao Paulo, Dept Microbiol Imunol Parasitol, Sao Paulo, BrazilWeb of ScienceConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)PRONEXNewton Fund/FAPEMIGCNPq: 444334/2014-9FAPEMIG: APQ-00827-15Elsevier Science BvUniversidade Federal de São Paulo (UNIFESP)2020-07-17T14:02:45Z2020-07-17T14:02:45Z2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion55-67https://dx.doi.org/10.1016/j.molbiopara.2017.01.005Molecular And Biochemical Parasitology. Amsterdam, v. 212, p. 55-67, 2017.10.1016/j.molbiopara.2017.01.0050166-6851https://repositorio.unifesp.br/handle/11600/54992WOS:000397374700008ark:/48912/001300001xxp3engMolecular And Biochemical ParasitologyAmsterdaminfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPCerqueira, Paula GonçalvesPassos-Silva, Danielle GomesVieira-da-Rocha, João PedroMendes, Isabela CeciliaOliveira, Karla Andrade deOliveira, Camila Franco Batista deVilela, Liza Figueiredo FelicoriNagem, Ronaldo Alves PintoCardoso, JoseaneNardelli, Sheila CristinaKrieger, Marco AurélioFranco, Gloria ReginaMacedo, Andrea MaraPena, Sergio Danilo JunhoSchenkman, Sergio [UNIFESP]Gomes, Dawidson A.Guerra-Sa, RenataMachado, Carlos Renato2022-02-08T11:53:21Zoai:repositorio.unifesp.br:11600/54992Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652022-02-08T11:53:21Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
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15,228081 |