SDS-PAGE seminal plasma proteins pattern and its correlation with gir breed semen freezability

This study was undertaken to determine whether bovine seminal plasma contained protein markers associated with semen freezability. Seminal plasma was obtained from ten Gir bulls of known fertility and freezability, set into two groups of High (up to 80% of viable sperm after thawing) and Low freezab...

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Detalhes bibliográficos
Autores: Roncoletta, Marcelo, Franceschini, Paulo Henrique, Lima, Vera Fernanda Martins Hossepian de, Rodrigues, Lúcia Helena, Oliveira, Marcelo Almeida, Silva, Carlos da
Formato: artículo
Estado:Versión publicada
Fecha de publicación:1999
País:Brasil
Recursos:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
Repositorio:Brazilian Journal of Veterinary Research and Animal Science
Idioma:portugués
OAI Identifier:oai:revistas.usp.br:article/5766
Acesso em linha:https://www.revistas.usp.br/bjvras/article/view/5766
Access Level:acceso abierto
Palavra-chave:Plasma seminal
Bovinos
Eletroforese
Congelamento
Sêmen
Seminal plasma
Cattle
Electrophoresis
Freezing
Semen
Descrição
Resumo:This study was undertaken to determine whether bovine seminal plasma contained protein markers associated with semen freezability. Seminal plasma was obtained from ten Gir bulls of known fertility and freezability, set into two groups of High (up to 80% of viable sperm after thawing) and Low freezability (less than 50%). SDS-PAGE of seminal plasma samples indicated that one protein (Mr =-20.3;-MW-=-61,800Da) predominated in high-freezability semen. These findings indicate that bull seminal plasma contains freezability-associated proteins which can be used as markers for predicting semen freezability.