Proteomic profile of seminal plasma and spermatozoa of donkeys of the Pêga breed and its relationship with assisted reproduction

Donkeys, along with their hybrids, mules and hinnies, resulting from breeding with mares, have a longstanding tradition of being used for labor-intensive tasks. Historically, these animals played pivotal roles in agriculture, warfare, trade, and transportation. However, with the advent of modernizat...

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Detalhes bibliográficos
Autor: Costa, Isabella Cristina Tolêdo Alves
Formato: tesis doctoral
Estado:Versión publicada
Fecha de publicación:2023
País:Brasil
Recursos:Universidade Federal de Viçosa (UFV)
Repositorio:LOCUS Repositório Institucional da UFV
Idioma:inglés
OAI Identifier:oai:locus.ufv.br:123456789/32079
Acesso em linha:https://locus.ufv.br//handle/123456789/32079
https://doi.org/10.47328/ufvbbt.2024.017
Access Level:acceso abierto
Palavra-chave:Jumentos - Reprodução
Jumentos - Infecundidade
Sêmen - Criopreservação
Marcadores bioquímicos
Proteínas
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oai_identifier_str oai:locus.ufv.br:123456789/32079
network_acronym_str BR
network_name_str Brasil
repository_id_str
dc.title.none.fl_str_mv Proteomic profile of seminal plasma and spermatozoa of donkeys of the Pêga breed and its relationship with assisted reproduction
Perfil proteômico do plasma seminal e dos espermatozoides de jumentos da raça Pêga e sua relação com a reprodução assistida
title Proteomic profile of seminal plasma and spermatozoa of donkeys of the Pêga breed and its relationship with assisted reproduction
spellingShingle Proteomic profile of seminal plasma and spermatozoa of donkeys of the Pêga breed and its relationship with assisted reproduction
Costa, Isabella Cristina Tolêdo Alves
Jumentos - Reprodução
Jumentos - Infecundidade
Sêmen - Criopreservação
Marcadores bioquímicos
Proteínas
Proteínas
title_short Proteomic profile of seminal plasma and spermatozoa of donkeys of the Pêga breed and its relationship with assisted reproduction
title_full Proteomic profile of seminal plasma and spermatozoa of donkeys of the Pêga breed and its relationship with assisted reproduction
title_fullStr Proteomic profile of seminal plasma and spermatozoa of donkeys of the Pêga breed and its relationship with assisted reproduction
title_full_unstemmed Proteomic profile of seminal plasma and spermatozoa of donkeys of the Pêga breed and its relationship with assisted reproduction
title_sort Proteomic profile of seminal plasma and spermatozoa of donkeys of the Pêga breed and its relationship with assisted reproduction
dc.creator.none.fl_str_mv Costa, Isabella Cristina Tolêdo Alves
author Costa, Isabella Cristina Tolêdo Alves
author_facet Costa, Isabella Cristina Tolêdo Alves
author_role author
dc.contributor.none.fl_str_mv Baracat-Pereira, Maria Cristina
http://lattes.cnpq.br/0564337669101490
Guimarães, José Domingos
Mendes, Tiago Antônio de Oliveira
Ramírez López, Camilo José
dc.subject.por.fl_str_mv Jumentos - Reprodução
Jumentos - Infecundidade
Sêmen - Criopreservação
Marcadores bioquímicos
Proteínas
Proteínas
topic Jumentos - Reprodução
Jumentos - Infecundidade
Sêmen - Criopreservação
Marcadores bioquímicos
Proteínas
Proteínas
description Donkeys, along with their hybrids, mules and hinnies, resulting from breeding with mares, have a longstanding tradition of being used for labor-intensive tasks. Historically, these animals played pivotal roles in agriculture, warfare, trade, and transportation. However, with the advent of modernization, this dynamic has shifted. In Europe, these animals have become more popular as pets, and some species even faced the brink of extinction. In contrast, Brazil hosts a significant population of undomesticated donkeys, especially in the Northeast. Recently, the demand for donkeys has seen a resurgence, primarily in China, due to the consumption of their milk and meat. Assisted reproduction would be an advantageous approach, as it is more cost-effective and practical than maintaining animals in the field. However, when cryopreserved, donkey semen suffers a drastic decrease in its fertilization capabilities. The first chapter of this thesis delved into discerning the differences between the proteomic profiles of cryopreserved and non-cryopreserved sperm. In cryopreserved samples, proteins associated with infertility were identified, such as SERPINE2 and SERPINC1, both linked to the loss of sperm capacitation, and PLAU, associated with reduced motility. A high concentration of nucleoporins, related to neurodegenerative diseases, was also observed. In contrast, non-cryopreserved sperm displayed a proteomic profile primarily oriented towards energy production and metabolism, reflecting the energetic demands required for maintaining sperm motility and viability. In the second chapter, the proteomic profile of donkey seminal plasma was explored, emphasizing the identification of potential infertility biomarkers. Compared to existing literature, CRISP3 and HSP1 were identified, proteins previously linked to infertility in stallions. Also, CRISP1, PTP an PKA have potencial as biomarkers. The proteomic profile also highlighted proteins connected to energy production, metabolism, and reproduction, suggesting an influential role of seminal plasma proteins in sperm viability during the reproductive process of donkeys. Keywords: Donkeys. Seminal plasma. Proteomics. Assisted reproduction. Cryopreserved semen.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-28
2024-01-30T13:58:44Z
2024-01-30T13:58:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv COSTA, Isabella Cristina Tolêdo Alves. Proteomic profile of seminal plasma and spermatozoa of donkeys of the Pêga breed and its relationship with assisted reproduction. 2023. 90 f. Tese (Doutorado em Bioquímica Aplicada) - Universidade Federal de Viçosa, Viçosa. 2023
https://locus.ufv.br//handle/123456789/32079
https://doi.org/10.47328/ufvbbt.2024.017
identifier_str_mv COSTA, Isabella Cristina Tolêdo Alves. Proteomic profile of seminal plasma and spermatozoa of donkeys of the Pêga breed and its relationship with assisted reproduction. 2023. 90 f. Tese (Doutorado em Bioquímica Aplicada) - Universidade Federal de Viçosa, Viçosa. 2023
url https://locus.ufv.br//handle/123456789/32079
https://doi.org/10.47328/ufvbbt.2024.017
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Viçosa
Bioquímica Aplicada
publisher.none.fl_str_mv Universidade Federal de Viçosa
Bioquímica Aplicada
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
_version_ 1853667087360196608
spelling Proteomic profile of seminal plasma and spermatozoa of donkeys of the Pêga breed and its relationship with assisted reproductionPerfil proteômico do plasma seminal e dos espermatozoides de jumentos da raça Pêga e sua relação com a reprodução assistidaJumentos - ReproduçãoJumentos - InfecundidadeSêmen - CriopreservaçãoMarcadores bioquímicosProteínasProteínasDonkeys, along with their hybrids, mules and hinnies, resulting from breeding with mares, have a longstanding tradition of being used for labor-intensive tasks. Historically, these animals played pivotal roles in agriculture, warfare, trade, and transportation. However, with the advent of modernization, this dynamic has shifted. In Europe, these animals have become more popular as pets, and some species even faced the brink of extinction. In contrast, Brazil hosts a significant population of undomesticated donkeys, especially in the Northeast. Recently, the demand for donkeys has seen a resurgence, primarily in China, due to the consumption of their milk and meat. Assisted reproduction would be an advantageous approach, as it is more cost-effective and practical than maintaining animals in the field. However, when cryopreserved, donkey semen suffers a drastic decrease in its fertilization capabilities. The first chapter of this thesis delved into discerning the differences between the proteomic profiles of cryopreserved and non-cryopreserved sperm. In cryopreserved samples, proteins associated with infertility were identified, such as SERPINE2 and SERPINC1, both linked to the loss of sperm capacitation, and PLAU, associated with reduced motility. A high concentration of nucleoporins, related to neurodegenerative diseases, was also observed. In contrast, non-cryopreserved sperm displayed a proteomic profile primarily oriented towards energy production and metabolism, reflecting the energetic demands required for maintaining sperm motility and viability. In the second chapter, the proteomic profile of donkey seminal plasma was explored, emphasizing the identification of potential infertility biomarkers. Compared to existing literature, CRISP3 and HSP1 were identified, proteins previously linked to infertility in stallions. Also, CRISP1, PTP an PKA have potencial as biomarkers. The proteomic profile also highlighted proteins connected to energy production, metabolism, and reproduction, suggesting an influential role of seminal plasma proteins in sperm viability during the reproductive process of donkeys. Keywords: Donkeys. Seminal plasma. Proteomics. Assisted reproduction. Cryopreserved semen.Jumentos, juntamente com seus híbridos burros e mulas resultantes do cruzamento com éguas, têm uma longa tradição de uso em tarefas que exigem força. Historicamente, esses animais desempenharam papéis fundamentais na agricultura, combate armado, comércio e transporte. No entanto, com a onda de modernização, essa dinâmica sofreu alterações. Na Europa, esses animais se tornaram mais comuns como pets, e algumas espécies chegaram à beira da extinção. Em contraste, o Brasil tem uma vasta população de jumentos não domesticados, especialmente no Nordeste. Recentemente, a demanda por jumentos ressurgiu, principalmente na China, devido ao consumo de seu leite e carne. A reprodução assistida seria uma abordagem altamente benéfica, pois é mais econômica e prática do que manter os animais em pasto, contudo, o sêmen de jumento, quando criopreservado, sofre uma drástica diminuição em sua capacidade de fertilização. O primeiro capítulo desta tese se dedicou a discernir as diferenças entre os perfis proteômicos de espermatozoides criopreservados e não criopreservados. Em amostras criopreservadas, identificou-se a presença de proteínas associadas à infertilidade, como SERPINE2, ligada à perda de capacitação espermática, e PLAU, associada à redução da motilidade. Também se observou uma alta concentração de nucleoporinas, relacionadas a doenças neurodegenerativas. Em contraste, os espermatozoides não criopreservados exibiram um perfil proteômico voltado principalmente para a produção de energia e metabolismo, refletindo as demandas energéticas para a manutenção da motilidade e viabilidade espermática. No segundo capítulo, explorou-se o perfil proteômico do plasma seminal de jumentos, com ênfase na identificação de potenciais biomarcadores de infertilidade. Comparando com dados da literatura, identificaram-se CRISP3 e HSP1, proteínas previamente associadas à infertilidade em garanhões. Também, CRISP1, PTP and PKA possuem potencial como biomarcadores. O perfil proteômico também revelou a prevalência de proteínas ligadas à produção de energia, metabolismo e reprodução, sugerindo um papel influente das proteínas do plasma seminal na viabilidade espermática durante o processo reprodutivo dos jumentos Palavras-chave: Jumentos. Plasma seminal. Proteômica. Reprodução assistida. Sêmen criopreservado.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorUniversidade Federal de ViçosaBioquímica AplicadaBaracat-Pereira, Maria Cristinahttp://lattes.cnpq.br/0564337669101490Guimarães, José DomingosMendes, Tiago Antônio de OliveiraRamírez López, Camilo José2024-01-30T13:58:44Z2024-01-30T13:58:44Z2023-09-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfCOSTA, Isabella Cristina Tolêdo Alves. Proteomic profile of seminal plasma and spermatozoa of donkeys of the Pêga breed and its relationship with assisted reproduction. 2023. 90 f. Tese (Doutorado em Bioquímica Aplicada) - Universidade Federal de Viçosa, Viçosa. 2023https://locus.ufv.br//handle/123456789/32079https://doi.org/10.47328/ufvbbt.2024.017enginfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVCosta, Isabella Cristina Tolêdo Alves2024-07-12T06:53:27Zoai:locus.ufv.br:123456789/32079Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T06:53:27LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
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