Cryprotectant effect of trehalose and low-density lipoprotein in extenders for frozen ram sperm

This study tested trehalose and low-density lipoprotein (LDL) as cryoprotectants in extenders for frozen ram semen. In the first experiment, the extenders were Tris, with 20% egg yolk (E1-1); E1-1 with 5% glycerol (E1-2); E1-1 with 100mM trehalose (E1-3); and E1-1 with 100mM trehalose and 5% glycero...

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Detalles Bibliográficos
Autores: Tonieto, Rafael Adolfo, Goulart, Karina Lemos, Gastal, Gustavo Desire Antunes, Schiavon, Raquel Schiavon, Deschamps, João Carlos, Lucia Júnior, Thomaz
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:Brasil
Institución:Universidade Federal de Pelotas (UFPEL)
Repositorio:Repositório Institucional da UFPel - Guaiaca
Idioma:inglés
OAI Identifier:oai:guaiaca.ufpel.edu.br:123456789/114
Acceso en línea:https://guaiaca.ufpel.edu.br/handle/123456789/114
Access Level:acceso abierto
Palabra clave:Extender
Frozen semen
Ram
Descripción
Sumario:This study tested trehalose and low-density lipoprotein (LDL) as cryoprotectants in extenders for frozen ram semen. In the first experiment, the extenders were Tris, with 20% egg yolk (E1-1); E1-1 with 5% glycerol (E1-2); E1-1 with 100mM trehalose (E1-3); and E1-1 with 100mM trehalose and 5% glycerol (E1-4). Sperm motility and membrane integrity of the E1-2, E1-3 and E1-4 extenders were greater than for E1-1 (P < 0.05), but acrosome integrity following cryopreservation did not differ. In the second experiment, the extenders were Tris, with 20% egg yolk and 100mMtrehalose (E2-1); Tris with 8% LDL and 5% glycerol(E2-2); Tris with 8% LDL and 100mM trehalose (E2-3); and Tris with 8% LDL, 100mM trehalose and 5% glycerol (E2-4). Sperm membrane integrity was lowest for the E2-1 extender (P < 0.05), but similar for extenders including LDL. Sperm motility post-thawing was highest for E2-2 and E2-3 extenders (P < 0.05), but acrosome integrity did not differ. Thus, extenders including trehalose and LDL as cryoprotectants recorded a post-thawing ram sperm quality similar to that achieved when using conventional cryoprotectants.

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