CARACTERIZAÇÃO QUÍMICA E AVALIAÇÃO DA ATIVIDADE LARVICIDA FRENTE AO Aedes aegypt do ÓLEO ESSENCIAL DA ESPÉCIE Citrus sinensis L. Osbeck (LARANJA DOCE)
Studies are conducted to find substances of vegetable origin, such as alternative chemical control to combat the dengue vector, once there is no validated vaccine for that. In this paper, from the extraction and analytical study of the essential oil of the fresh peel of Citrus sinensis L.Osbeck was...
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| Formato: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2013 |
| País: | Brasil |
| Recursos: | Universidade Federal do Maranhão (UFMA) |
| Repositorio: | Biblioteca Digital de Teses e Dissertações da UFMA |
| Idioma: | portugués |
| OAI Identifier: | oai:tede2:tede/954 |
| Acesso em linha: | http://tedebc.ufma.br:8080/jspui/handle/tede/954 |
| Access Level: | acceso abierto |
| Palavra-chave: | Óleo essencial Citrus sinensis L.Osbeck d-Limoneno Atividade larvicida Aedes aegypti Essential oil d-limonene Larvicidal activity CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
| Resumo: | Studies are conducted to find substances of vegetable origin, such as alternative chemical control to combat the dengue vector, once there is no validated vaccine for that. In this paper, from the extraction and analytical study of the essential oil of the fresh peel of Citrus sinensis L.Osbeck was possible to analyze the larvicidal effect of the oil against third stage larvae of Aedes aegypti (Linnaeus, 1762). The oil was extracted quantitatively by steam distillation process using Clevenger type apparatus. The physico-chemical properties of essential oil were determined (density, refractive index, solubility, color and appearance). The oil was characterized analytically by infrared (FTIR) and gas chromatography-mass spectrometry (GC-MS),thus identifying its major compound. LC50 from the oil was calculated from the Reed-Muench method (1938) and Pizzi (1950), respectively. The yield of oil was 2.47% m/m.Through spectroscopic techniques used,it was possible to identify 15 compounds, justifying presence of d-limonene as a major constituent. The essential oil exhibited LC50 of 99.014 (± 2.098) μg.mL-1 and the standard d-Limonene 126.037 (± 2.092) μg.mL-1. The results indicate that the essential oil Citrus sinensis L. Osbeck comprises substances with larvicidal effect against mosquito Aedes aegypti. |
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